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32,115 results

Showing: 5481 - 5520 of 32115 results

  2. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  10. O-TREM2-2

    Plasmid
    #166545
    Purpose
    scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166545-plasmid-map-sequence-id-329237

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  20. NNMT

    Plasmid
    #40734
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NNMT (NNMT Human)
    Tags
    His Thrombin
    Expression
    Bacterial
    Mutation
    K100A:E101A:E103A
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. CYP51A1 (3I3K)

    Plasmid
    #25161
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP51A1_cw_09 (CYP51A1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 54-502
    Available Since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  31. pGEX4T1 SHP1 WT

    Plasmid
    #8578
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Expression
    Bacterial
    Available Since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  33. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

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  35. pBP-P_tetR-PTet*

    Plasmid
    #204077
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    tetR repressor and PTet* tetracycline-inducible promoter
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204077-plasmid-map-sequence-id-413892

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  37. pSU313

    Plasmid
    #169695
    Purpose
    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    Tags
    1xFLAG tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169695-plasmid-map-sequence-id-334516

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  39. FKBP8

    Plasmid
    #25609
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FKBP8 (FKBP8 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    FKBP-type PPIase Domain, residues 90-205
    Available Since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25609-plasmid-map-sequence-id-454823

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Showing: 5481 - 5520 of 32115 results