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Showing: 5481 - 5520 of 29646 results

  1. pSucCD2

    Plasmid
    #83324
    Purpose
    expresses E. coli succinyl-CoA synthetase
    Depositor
    Robert Hausinger
    Insert
    sucCD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83324-plasmid-map-sequence-id-160476

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  4. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  6. pRBC-sfGFP-134/150TAG

    Plasmid
    #174077
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoter
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    D134TAG + N150TAG
    Promoter
    T7
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174077-plasmid-map-sequence-id-345699

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  11. YWHAQ

    Plasmid
    #38931
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAQ (YWHAQ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pWT009d

    Plasmid
    #107888
    Purpose
    R6 plasmid. Contains PBAD-EGFP and is part of part of the CAMERA system for rewritable multi-event analog recording in bacteria
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PBAD-EGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107888-plasmid-map-sequence-id-207298

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  21. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  25. USP21 (3I3T)

    Plasmid
    #25147
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp21.209.563 (USP21 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. Dead Streptavidin-SpyCatcher

    Plasmid
    #59547
    Purpose
    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers
    Depositor
    Mark Howarth
    Article
    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-
    Insert
    Dead-dloop-Streptavidin-SpyCatcher
    Use
    Tags
    N-terminally shortened SpyCatcher
    Expression
    Bacterial
    Mutation
    PolyAsp in L3/4 loop for ion-exchange
    Promoter
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pGEX4T3-Stx3(1-265)

    Plasmid
    #100573
    Purpose
    Expresses cytoplasmic domain of Stx3(1-265) with a GST tag on the N-term.
    Depositor
    Thomas Weimbs
    Insert
    Stx3-1-265 (Stx3 Rat)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    No transmembrane domain, residues 4-264 present.
    Promoter
    tac
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    100573-plasmid-map-sequence-id-192872

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  35. pHCL151

    Plasmid
    #134455
    Purpose
    TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    linker-mScarlet-I for C-terminal fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    plac
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134455-plasmid-map-sequence-id-270547

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  36. pET-His6-Sumo-CfaC-MBP

    Plasmid
    #126018
    Purpose
    Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-CfaC-MBP
    Use
    Tags
    6xHis-SUMO and HA-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126018-plasmid-map-sequence-id-248773

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  37. SBC015866

    Plasmid
    #226280
    Purpose
    Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.
    Depositor
    Rainer Breitling
    Article
    Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.
    Inserts
    4'-phosphopantetheinyl transferase Sfp
    Cinnamyl alcohol dehydrogenase
    Carboxylic acid reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226280-plasmid-map-sequence-id-452181

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  39. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Use
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

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Showing: 5481 - 5520 of 29646 results