32,776 results

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pJL1-YPet

Plasmid

#102633

Purpose

In vitro expression of YPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

YPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD-LBD

Plasmid

#171237

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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His-huH2Ax-huH2B

Plasmid

#197273

Purpose

Human (hu) histones H2Ax and H2B inserted into a plasmid

Depositor

Mark Glover

Article

Panigrahi et al Nucleic Acids Res. 2025 Oct 14;53(19):gk

Inserts

huH2Ax
huH2B

Tags

Histidine tag and No tag

Expression

Bacterial

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-mScarlet-2I

Plasmid

#160463

Purpose

Encodes a variant of mScarlet-I that displays reduced cytotoxicity in bacteria

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

mScarlet-2I

Expression

Bacterial

Promoter

T5

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET-22b(+) pCopA RBS30 sfgfp

Plasmid

#226371

Purpose

Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

sfGFP

Tags

6x HisTag

Expression

Bacterial

Promoter

copA promoter (pCopA) and RBS30 regulation

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pIS003

Plasmid

#194014

Purpose

This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

Depositor

Timothy Whitehead

Article

Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

Insert

C16 Ex4 XTR f1

Expression

Bacterial

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG GST EGFP

Plasmid

#236736

Purpose

Bacterial expression plasmid containing GST-tagged EGFP

Depositor

Melina Fan, Addgene Research Program

Insert

EGFP

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

April 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCR-Zeo

Plasmid

#158709

Purpose

For cloning and constitutive expression of crRNAs in mycobacterium

Depositor

Yi-Cheng Sun

Article

Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

crRNA

Use

CRISPR and Synthetic Biology; Mycobacteria expres…

Expression

Bacterial

Promoter

Hsp60

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Etanercept

Plasmid

#182421

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-Etanercept (TNFRSF1B Human)

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-ecfp-a

Plasmid

#111619

Purpose

eCFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Article

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-ecfp

Expression

Bacterial

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDUET-1-alpha-synuclein-mCerulean3-His6

Plasmid

#110061

Purpose

For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha-synuclein (SNCA Human)

Tags

6 x His and mCerulean3

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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PBAD-his6-prkA-pACYC184

Plasmid

#41041

Depositor

Ichiro Matsumura

Article

Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

Insert

prkA

Tags

6xHis and T7 epitope tag

Expression

Bacterial

Promoter

PBAD

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PBBa_J23106-geneIII

Plasmid

#134352

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene III

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHEN2 F8-2

Plasmid

#186033

Purpose

Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

Depositor

Isabelle Landrieu

Article

Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

Insert

VHH F8-2

Tags

6His and Myc

Expression

Bacterial

Promoter

lac

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCR4 hNFATc1-EE

Plasmid

#17857

Depositor

Jerry Crabtree

Article

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc1 (NFATC1 Human)

Tags

EE

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Expression

Bacterial

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pFA6a-SNAP-KanMX6

Plasmid

#87024

Purpose

Tag S. pombe genes in C terminal with SNAP

Depositor

Julien Berro

Article

Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

Insert

SNAP

Expression

Bacterial and Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pSR1038

Plasmid

#208737

Purpose

Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PCap

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCap

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pET16better_H10_HRV3C_chitinase

Plasmid

#175091

Purpose

Expresses B. licheniformis chitinase with an N terminal 10X His tag and HRV-3C cleavage site in E. coli.

Depositor

Jay C Dunlap

Article

Bartholomai et al Fungal Genet Biol. 2021 Aug 20;156:10361

Insert

chitinase

Tags

10X His, HRV-3C

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L Hu IL-15-His tag

Plasmid

#183256

Purpose

Bacterial expression of human IL-15 with N-terminal His tag

Depositor

Alexander McLellan

Insert

IL-15 (IL15 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET30a(+)-PE2

Plasmid

#180015

Purpose

Expressing PE2 protein in E.coli for protein purification

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

PE2

Tags

6xHis

Expression

Bacterial

Promoter

Lac operator-controlled T7 promoter

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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