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Plasmid#45152DepositorInsertLon
UseSynthetic BiologyTagsssRA degradation (LVA)ExpressionBacterialMutationPromoterPLlacO-1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorInsertgfpmut2 (NEWENTRY Synthetic)
UseTagsExpressionBacterialMutationPromoterPxyl(s)Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJDC60
Plasmid#157780PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.DepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterL5Available sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PE_26: Para_Lac_Tet_eG::sfYFP
Plasmid#104887PurposePE_26: Para_Lac_Tet_eG::sfYFPDepositorInsertHybrid Promoter Para_Lac_Tet_eG
UseTagsExpressionBacterialMutationPromoterPara_Lac_Tet_eGAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BCAR1-1/1
Plasmid#91418PurposeProtein expression and purification of human SH3 domain construct BCAR1-1/1DepositorInsertBCAR1-1/1 (BCAR1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-ER-MT-P2A
Plasmid#214892PurposeExpresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sitesDepositorInsertSPLICSFAST-ER-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB-His-MBP-mGSDMD
Plasmid#123365PurposeExpression of full-length mouse GSDMD in E. coliDepositorInsertGSDMD (Gsdmd Mouse)
UseTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorInsertCRYAA (CRYAA Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-Rad23A_pET11a
Plasmid#169124Purposeexpression of proteinDepositorInserthRad23A (RAD23A Human)
UseTagsExpressionBacterialMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-clbK
Plasmid#114152PurposeExpresses ClbKDepositorInsertclbK
UseTags6xhis-ThrombinExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbI
Plasmid#114151PurposeExpresses ClbIDepositorInsertclbI
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbF
Plasmid#114148PurposeExpresses ClbF-cHisDepositorInsertClbF
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-clbC
Plasmid#114145PurposeExpresses ClbC-nHisDepositorInsertclbC
UseTags6xHis-thrombin-T7 tagExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbE
Plasmid#114147PurposeExpresses ClbE-cHisDepositorInsertClbE
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbD
Plasmid#114146PurposeExpresses ClbD-cHisDepositorInsertClbD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbG
Plasmid#114150PurposeExpresses ClbGDepositorInsertclbG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACDura3
Plasmid#138711PurposeExpresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.DepositorInsertura3 gene (URA3 Budding Yeast)
UseTagsExpressionBacterialMutationoptimized ura3 gene for expression in Escherichia…PromoterpEM7 promoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbBpks
Plasmid#114154PurposeExpresses the PKS module of ClbBDepositorInsertclbB
UseTags6xHisExpressionBacterialMutationOnly contains the PKS module of ClbBPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-eGFP
Plasmid#176862PurposeExpresses Glt-1 fused with GFP at the C-terminus in astrocytesDepositorInsertGlt1-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropVn
Plasmid#184873PurposeChromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.DepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
3521 pET15b Bid p15
Plasmid#8782DepositorInsertBid p15 (Bid Mouse)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSARSF53-110
Plasmid#61813PurposeTvoVMAN11 inteinDepositorInsertTvoVMAN11 intein
UseTagsH6-Smt3ExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-98
Plasmid#61827PurposeTvoVMAdN11 inteinDepositorInsertTvoVMAdN11 intein
UseTagsGB1-H6ExpressionBacterialMutationPromoterpBADAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB
Plasmid#176587PurposeBacterial expression of His14-MBP-TEV-AmaTnpBDepositorInsertsMBP tag
TnpB
UseTagsHis14 tagExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVRK1 (VRK1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertNEK7 (NEK7 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKRR5
Plasmid#149468PurposeBroad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertRhaS-RhaR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Alpha1
Plasmid#118044PurposeGoldenBraid2.0 Alpha 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpG-His
Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/hGGA3(GAT)
Plasmid#79436PurposeExpresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coliDepositorInsertGGA3 (GGA3 Human)
UseTagsGSTExpressionBacterialMutationamino acids 107-286PromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbB5k-GFP
Plasmid#35354DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsNot1iso2(MCSI)_O
Plasmid#147088PurposeBacterial Expression of HsNot1iso2DepositorInsertHsNot1iso2 (CNOT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mCardinal2
Plasmid#52631Purposebacterial expression vector with far-red FP mCardinal2DepositorInsertmCardinal2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -