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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBJ3

Plasmid

#167132

Purpose

A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Frr promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Article

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_RAD51_Rad51

Plasmid

#110031

Purpose

Protein expression and purification of RAD51_Rad51

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAD51_Rad51 (RAD51 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-EV

Plasmid

#202466

Purpose

Empty vector of pSMART-HCKan

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196)-Strep

Plasmid

#196208

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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hPU.1ΔN165-pET28b

Plasmid

#199796

Purpose

Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

Depositor

Gregory Poon

Article

Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

Insert

spi1 (SPI1 Human)

Tags

None

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GST-OPTN

Plasmid

#171424

Purpose

For the purifcation of OPTN protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

OPTN (OPTN Human)

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-ptetO::bac::gfp

Plasmid

#217882

Purpose

expresses the bac-BGC in bacterial cells, produces bacillamide D

Depositor

Tobias A. M. Gulder

Article

Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

Inserts

Dehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase

Expression

Bacterial

Promoter

ptetO

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mu-IFNg-pCIneo

Plasmid

#163517

Purpose

Expresses murine IFN gamma in mammalian cells

Depositor

Thomas Weber

Insert

murine-IFN gamma (Ifng Mouse)

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pU1_000

Plasmid

#186773

Purpose

* Engineered U1 snRNA plasmid for insertion of guide sequences.

Depositor

Eugene Yeo

Article

Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U1 Promoter

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Plasmid

#101215

Purpose

Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K848A/K1003A/R1060A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K848A, K1003A and R1060A

Promoter

T7

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCAT218

Plasmid

#134886

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-88)-Strep

Plasmid

#196214

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-moxMaple3

Plasmid

#120871

Purpose

Bacterial expression of moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Tags

His

Expression

Bacterial

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK 14-3-3 sigma GST

Plasmid

#11944

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 sigma (SFN Human)

Tags

GST

Expression

Bacterial

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-5FLAG-KanMX6

Plasmid

#15983

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Type

Empty backbone

Tags

5xFlag

Expression

Bacterial

Available Since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pET-45b-mCherry

Plasmid

#145279

Purpose

Bacterial Expression of 6xHis mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-DmPABPC1_J

Plasmid

#146647

Purpose

Bacterial Expression of DmPABPC1

Depositor

Elisa Izaurralde

Insert

DmPABPC1 (pAbp Fly)

Expression

Bacterial

Mutation

G26S relative to NM_166265.2/NP_725750.1

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-Lysozyme fAb

Plasmid

#214951

Purpose

Bacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusions

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion

Tags

VNp

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-37Q

Plasmid

#84351

Purpose

Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

Plasmid

#228644

Purpose

Bacterial expression of the D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus D68 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHAT2

Plasmid

#112583

Purpose

Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pJ2072.2_c1

Plasmid

#140675

Purpose

CRISPRlator control (“open ring”)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

CRISPRlator control (“open ring”)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pG1C

Plasmid

#71740

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBadcrtEBI_13

Plasmid

#63811

Purpose

expresses crtEBI genes for neurosporene production

Depositor

Howard Salis

Article

Farasat et al Mol Syst Biol. 2014 Jun 21;10:731. doi:

Insert

crtEBI operon

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

PBAD

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET21 E2F1

Plasmid

#90313

Purpose

Expresses human E2F1 in bacteria

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 (E2F1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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