Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,115 results

Showing: 5921 - 5960 of 32115 results

  2. p404GALS

    Plasmid
    #17435
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    GALS promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17435-plasmid-map-sequence-id-8609

    Enlarge

  3. C14orf169

    Plasmid
    #39114
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    C14orf169 (C14orf169 Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    T. J. Kappock
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72391-plasmid-map-sequence-id-133988

    Enlarge

  13. pQCascade-array1

    Plasmid
    #140623
    Purpose
    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140623-plasmid-map-sequence-id-306003

    Enlarge

  18. XX01CAPN1A-c001

    Plasmid
    #175491
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    CAPN1 / CAPNS1 (CAPN1 Human)
    Tags
    AviTag and His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175491-plasmid-map-sequence-id-353165

    Enlarge

  22. pOPS0381

    Plasmid
    #133231
    Purpose
    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
    Depositor
    Philip Poole
    Article
    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117
    Insert
    constructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133231-plasmid-map-sequence-id-282538

    Enlarge

  27. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

    Enlarge

  29. TARS_pNIC-Bio3

    Plasmid
    #153057
    Purpose
    Expression construct for producing Avi tagged Threonyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    TARS (TARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153057-plasmid-map-sequence-id-304477

    Enlarge

  33. PCBD2A-c103

    Plasmid
    #53624
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    PCBD2A
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pET28a(+)/FbGH17A

    Plasmid
    #100911
    Purpose
    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli
    Depositor
    Jan-Hendrik Hehemann
    Insert
    FbGH17A
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    100911-plasmid-map-sequence-id-193697

    Enlarge

  35. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Nematode, Human)
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

    Enlarge

Showing: 5921 - 5960 of 32115 results