31,059 results
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Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEXndoS
Plasmid#44655DepositorAvailable SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Cre-6xHis
Plasmid#62939PurposeExpression of Cre-6xHis in bacterial of cellsDepositorInsertCre recombinase
Tags6xHis tagExpressionBacterialPromoterT7Available SinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP
Plasmid#200277PurposeCharacterisation of the sialic acid-responsive circuit with fluorescent reporterDepositorInsertsCadC
NanR
ExpressionBacterialAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IRP1-Halo
Plasmid#175305PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV297
Plasmid#204820PurposeAMA1 plasmid with amdS selection markerDepositorInsertamdS
UseFungal expressionExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans trpC promoterAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNGFP-BC
Plasmid#161956PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGFP-BC
Plasmid#161959PurposeUsed for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).DepositorTypeEmpty backboneTagsGFPuv His8ExpressionBacterialPromoterT7Available SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMB-Tl-ADPHK
Plasmid#237469PurposeExpression of His-tagged Thermococcus litoralis ADP hexokinaseDepositorInsertADP hexokinase
TagsC-terminal 10 His-tag and N-terminal strep tagExpressionBacterialPromoterT7Available SinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETCK3 TGP-E
Plasmid#236255PurposeExpresses TGP-E, a thermostable fluorescent proteinDepositorInsertThermal Green Protein Q66E (TGP-E)
TagsHis tagExpressionBacterialPromoterlac operonAvailable SinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2WWE
Plasmid#234210PurposeHeterologous protein expression of PI3K-C2-gamma in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1
Plasmid#184556PurposeKnockdown of murine KRAS gene by targeting the promoter region via CRISPRi systemDepositorInsertKirsten rat sarcoma virus (Kras Mouse)
UseCRISPR, Lentiviral, and Mouse TargetingTagsFlagExpressionBacterialAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving
Plasmid#204811PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
BPK2014-Cas12i-Max
Plasmid#188276Purposeprokaryotic expressionDepositorInsertCas12i-Max
UseCRISPRExpressionBacterialMutationnonePromoterT7Available SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromotercumate-inducibleAvailable SinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE-PalkB-GFP
Plasmid#44381DepositorInsertsGFP
PalkB
UseSynthetic BiologyExpressionBacterialPromoterPalkBAvailable SinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-mouse cyclin D2
Plasmid#86791Purposebacterial expression of mouse cyclin D2DepositorInsertcyclin D2 (Ccnd2 Mouse)
ExpressionBacterialAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTW357b
Plasmid#227930PurposeLow-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632SAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b+His SUMO PPM1H
Plasmid#207333PurposeBacterial expression of His SUMO PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p2attB-FST344-HA-CPL
Plasmid#131460PurposeEncodes human Follistatin proteinDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMyCA
Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAceteamidaseAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTTN-1/1
Plasmid#91550PurposeProtein expression and purification of human SH3 domain construct CTTN-1/1DepositorInsertCTTN-1/1 (CTTN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH
Plasmid#26117PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTagsHisExpressionBacterialPromoterT7-lacOAvailable SinceSept. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5X-ELP[AV-60]
Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
TagsHis6 and malE-Factor XaExpressionBacterialPromotertacAvailable SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Nkx2.2
Plasmid#64840PurposeA lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.DepositorAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFCM1
Plasmid#64948Purposesource of CmR FRT cassetteDepositorInsertFRT-cat-FRT
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ov-iPGM
Plasmid#180245PurposeBacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10DepositorInsertOnchocerca volvulus iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a Wb-iPGM
Plasmid#180246PurposeBacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10DepositorInsertWuchereria bancrofti iPGM-10His
TagsHisExpressionBacterialPromoterT7Available SinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a Ce-iPGM-bio
Plasmid#162559PurposeExpresses C. elegans iPGM containing a C-terminal biotinylation site and His tagDepositorInsertC. elegans iPGM bio-6His (ipgm-1 Nematode)
Tags6His tag, T7 tag, Thrombin cleavage site, and bio…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a Bm-iPGM-bio
Plasmid#162565PurposeExpresses B. malayi iPGM containing a C-terminal biotinylation site and His tagDepositorInsertB. malayi iPGM bio-6His
Tags6His tag, T7 tag, biotinylation sequence, and thr…ExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BRD7 (BRD7A-c005)
Plasmid#98245PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRD7A (BRD7 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00439:Bromodomain 136-223PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
Tags6His and mycExpressionBacterialPromoterpBADAvailable SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSt-KO
Plasmid#44566Purposefor generation of knockout of mycobacterial genesDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorExpressionBacterialAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEK19
Plasmid#210578PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdBIP1proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only