32,871 results
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pET28-mE1
Plasmid#32534DepositorAvailable SinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT2/BH
Plasmid#26556DepositorInsertpT2/BH
ExpressionBacterialAvailable SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
Tags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH2B_mCherry_IRES_puro2
Plasmid#21045DepositorAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP_NbALFA
Plasmid#136626PurposePlasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusionDepositorInsertEGFP-NbALFA
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
ExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
TagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTNT-B18R
Plasmid#58978Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNsDepositorInsertB18R
ExpressionBacterialPromoterT7/Sp6Available SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
ExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pPhiC31o
Plasmid#13794PurposeExpression of mouse codon-optimized PhiC31 recombinaseDepositorInsertPhiC31o
ExpressionBacterialAvailable SinceJan. 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR11 CA NFAT1
Plasmid#11792DepositorInsertNFAT1 (Nfatc2 Mouse)
TagsHAExpressionBacterialMutationConstitutively active: multiple serine to alanine…Available SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialPromoterT7lacAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY001 (pFnCpf1_full)
Plasmid#69973PurposeExpresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.DepositorInsertFnCpf1 locus
UseCRISPRExpressionBacterialAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EZH2
Plasmid#28060DepositorAvailable SinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-(pos36)GFP
Plasmid#62937PurposeExpression of 6xHis-(+36)GFP in bacterial cellsDepositorInsert(pos36)GFP
Tags6xHis tagExpressionBacterialPromoterT7Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only
