29,771 results
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Plasmid#103993PurposePlasmid encoding moxVenusDepositorInsertmoxVenus
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Medium-FT
Plasmid#31917DepositorInsertMedium-FT
UseTagsHisExpressionBacterialMutationM18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
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BPV00554 pET21GG2-His(6)-BECN(248-450)
Plasmid#136685PurposeExpresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 ECD/BARA (BECN1 Human)
UseTagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
SIRT5-pET15b
Plasmid#81030PurposeBacterial expression of Human WT SIRT5 proteinDepositorInsertSirt5 (SIRT5 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
UseTags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPro24(s)-gfp
Plasmid#17813DepositorInsertGFPuv
UseSynthetic BiologyTagsExpressionBacterialMutationD234N compared to AF007834PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJUMP28-1A(sfGFP)
Plasmid#126976PurposeLevel 1 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC37
Plasmid#111618PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosaDepositorInsertPpqsA-gfp(ASV)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTBXh-1
Plasmid#26077DepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 Parkin RBR
Plasmid#31255DepositorInsertParkin (Prkn Rat)
UseTagsGSTExpressionBacterialMutationRBR domain, residues 217-465PromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)1
Plasmid#127094PurposeBacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertmCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterunknownAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJH1260
Plasmid#36913DepositorInsertsScURA3
ScURA3
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-merR_O
Plasmid#127023PurposeBasic Part O- ORF; merR; transcription factorDepositorInsertPart merR_o
UseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
803 pGEX2T PTEN
Plasmid#10784DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProC.gfp
Plasmid#112517PurposeProC driven gfpmut3 expression with synTTDepositorInsertProC gfpmut3 TT
UseTagsExpressionBacterialMutationPromoterProCAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp3_Unique
Plasmid#167258PurposeBacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)DepositorInsertNsp3 (residues 413-676) (ORF1ab SARS-CoV-2)
UseTagsHis6-TEV siteExpressionBacterialMutationnonePromoterAvailable sinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBADM11-HMGR
Plasmid#108946Purposeheterologous expression of HMGR of S. solfataricus in E. coliDepositorInsertHMG-CoA reductase (HMGR)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro2GFP
Plasmid#82366PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro2GFP
UseTags6xHISExpressionBacterialMutationC48S/S65T/Q80R/S147C/Q204CPromoterT7Available sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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6C1A (MBD2)
Plasmid#119958PurposeBacterial expression for structure determinationDepositorInsertMBD2 (MBD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
UseTagsExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2
Plasmid#111086PurposeFor production of recombinant siRNAs (pro-siRNAs) in E. coli.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCORE-Kp53
Plasmid#72241PurposeContains the CORE cassette with KanMX4 and human p53 gene markersDepositorInsertsUseTagsExpressionBacterialMutationV122A mutationPromoterAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB085d
Plasmid#79209PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
S9_attL1-EGFP-C-term-attL2
Plasmid#128473PurposeEGFP entry cloneDepositorInsertEGFP entry clone
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRSETB PKA Cat
Plasmid#14920DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
MP-K12
Plasmid#69656PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA dinB
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL630
Plasmid#167836PurposeEncodes OptoLAC3 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ20
Plasmid#65027PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pHH0103_BCL2L1_BH4+Bcl-2
Plasmid#109993PurposeProtein expression and purification of BCL2L1_BH4+Bcl-2DepositorInsertBCL2L1_BH4+Bcl-2 (BCL2L1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAN-NOR
Plasmid#62306Purposesynthetic circuit: NOR gateDepositorInsertsA2NT sgRNA
A2NT sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPPhlF and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS5
Plasmid#145348PurposeCell-free/ mammalian expression vector for Zika virus NS5 protein with N-term eGFPDepositorInsertZIKV NS5
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX3x-hRanBP2-(IR1+M)
Plasmid#53140PurposeE.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activityDepositorInsertRanBP2 fragment (RANBP2 Human)
UseTagsGSTExpressionBacterialMutationfragment (amino acids 2631-2711)PromotertacAvailable sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
C163
Plasmid#141127PurposeExpresses sfCFP under lac promoterDepositorInsertsfCFP
UseTagsssrA degradation tagExpressionBacterialMutationPromoterengineered lac promoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
C164
Plasmid#141128PurposeExpresses sfYFP under lac promoterDepositorInsertsfYFP
UseTagsssrA degradation tagExpressionBacterialMutationPromoterengineered lac promoterAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pYC1876
Plasmid#158722PurposeHelper plasmid pYC1640 for C-terminal tagging with degradation tag ssrADepositorInsertssrA
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only