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32,115 results

Showing: 6401 - 6440 of 32115 results

  6. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130637-plasmid-map-sequence-id-255021

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  9. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  10. pGEX-6P-1-GST-CD(Cbx7)

    Plasmid
    #82525
    Purpose
    Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 7 (CBX7 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    CD(Cbx7) ; amino acids 1–62
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82525-plasmid-map-sequence-id-156818

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  24. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85497-plasmid-map-sequence-id-169854

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  32. pKD233.7-3

    Plasmid
    #90955
    Purpose
    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Expression
    Bacterial
    Promoter
    Bba_J23114, PltetO-1, and PttrB185-269
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    90955-plasmid-map-sequence-id-178326

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  34. g5s pD42

    Plasmid
    #117452
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Tags
    V5-His6
    Expression
    Bacterial
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117452-plasmid-map-sequence-id-226811

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  35. pACyR1

    Plasmid
    #172565
    Purpose
    Inducible gene expression, pACYC origin, CymRAM repressor, PCymRC promoter, mRFP, Chloramphenicol Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    CymRAM-mRFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PCymRC
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    172565-plasmid-map-sequence-id-347262

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  36. pMTS-hFluc-HA-GFP11

    Plasmid
    #177726
    Purpose
    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminus
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    hFluc
    Tags
    GFP11, HA, and Mitochondrial Targeting Sequence
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177726-plasmid-map-sequence-id-352620

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Showing: 6401 - 6440 of 32115 results