32,671 results
-
Plasmid#196248PurposePlasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitorDepositorInsertattL4-RecApro-dCas9-CDA1-UGI-attR1
ExpressionBacterialPromoterRecA promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA
Plasmid#183027PurposeB6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy numberDepositorInsertH2B-sfGFP-T2A-tdTomato
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR2F2_Hormone-recep
Plasmid#109826PurposeProtein expression and purification of NR2F2_Hormone-recepDepositorInsertNR2F2_Hormone-recep (NR2F2 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-p47
Plasmid#21268DepositorAvailable SinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b LFn-Fla
Plasmid#84871PurposeBacterial expression of LFn-Fla fusionDepositorInsertsLFn
Fla
TagsHisExpressionBacterialPromoterT7Available SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT1
Plasmid#53557Purposebacterial expression of IFIT1DepositorAvailable SinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKERep
Plasmid#211826PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PLlacO-1-CImf
Plasmid#40126DepositorInsertLac inducible lambda repressor
UseSynthetic BiologyTagsmf-lon ssrA tagExpressionBacterialAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_Nef_MCS2
Plasmid#66079PurposeSingle vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertNef
Tags6xHis and HRV 3C SiteExpressionBacterialPromoterT7-lacOAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-3_pET29b
Plasmid#215435PurposeExpression construct for UMG-SP-3 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-3
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NfPolyA_pET21b
Plasmid#215413PurposeExpression construct for NfPolyA gene, codon optimized for expression in E. coliDepositorInsertNfPolyA
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNS207
Plasmid#35041DepositorInsertcytochrome b562
ExpressionBacterialPromoterlacAvailable SinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
enz.083-NMT1
Plasmid#154047PurposeExpresses NMT1 in E. coliDepositorAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSU315
Plasmid#169697PurposeTemplate plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xHA tagExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH474_pTech_Para-supP
Plasmid#163718PurposeArabinose-inducible expression of supPDepositorInsertsupP
ExpressionBacterialAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b::mAID
Plasmid#15923DepositorAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2E
Plasmid#75291PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable SinceJuly 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-CDO1
Plasmid#31292DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUMV19
Plasmid#67161Purposeinducible operation of the mevalonate pathway in E. coliDepositorInsertthe mevalonate pathway genes
ExpressionBacterialPromoterlacZAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
ExpressionBacterialPromoterBBa_J23119Available SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectPromoterPolyhedrin promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS-dTomato
Plasmid#117391PurposeTn7 tagging vector pTn7xTS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
Tags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104Available SinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mMaple3-V157I
Plasmid#98577Purposephotoconvertible fluorescent protein mMaple3-V157I in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmMaple3-V157I
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-153
Plasmid#118535PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-62 pComb3xss anti-cFos nanobody
Plasmid#145835PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-62 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X71-527: His6-tev-Hs.CALM1(1-80)
Plasmid#159694PurposeE. coli protein expression of His6-tev-Hs.CALM1(1-80)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOSV805
Plasmid#126598Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5060
Plasmid#128716PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcN-hopN1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP6450
Plasmid#128731PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAD1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pBAD33.1-DpnII
Plasmid#127413PurposeExpresses DpnII restriction endonuclease in bacteriaDepositorInsertDpnII
ExpressionBacterialPromoterpBADAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSumo-His6-SUMO-AtTPR1(1-209)
Plasmid#177858PurposeBacterial Expression of TPR1DepositorAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a-PcAad1
Plasmid#163039Purposeexpression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporiumDepositorInsertaryl-alcohol dehydrogenase
TagsGST and His6ExpressionBacterialAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
TagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINTRX
Plasmid#41116DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-4
Plasmid#13001DepositorInsertTSP-4 (THBS4 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDIV298
Plasmid#204957PurposeAMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection markerDepositorInsertsMad7
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124K wgMDH
Plasmid#204258PurposeBacterial expression of R124K wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124KAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R124Q wgMDH
Plasmid#204259PurposeBacterial expression of R124Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR124QAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130E wgMDH
Plasmid#204267PurposeBacterial expression of R130E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
Tags6X HisExpressionBacterialMutationR130EAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST2-Rabex5 (9-73)
Plasmid#17630DepositorInsertRabex5 (9-73) (RABGEF1 )
TagsGSTExpressionBacterialMutationUbiquitin-binding domain: residues 9-73Available SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)
Plasmid#159698PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB223
Plasmid#170013PurposeEncodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
C18G
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only