31,059 results
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Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42
Plasmid#153525PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#42DepositorInsertSb#42
ExpressionBacterialPromoterpBADAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_H2_beta_GM
Plasmid#184871PurposepSwap plasmid part with H2_beta homology arms and gentamycin cassetteDepositorInsertlacZalpha,ccdB
ExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
HBT-GCaMP6-HA
Plasmid#107433PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertGCaMP6S
TagsHAExpressionBacterialAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
EPHA5
Plasmid#25144PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 6, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL4529 (pTnpA)
Plasmid#210869PurposeExpresses GstTnpA from pLac promoterDepositorInsertGstTnpA
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL5200 (pDonor_ISGst3)
Plasmid#210875PurposeHarbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458DepositorInsertISGst3
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
STK25
Plasmid#39075PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorInsertPropionate inducible promoter
UseSynthetic BiologyExpressionBacterialPromoterprpAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express
Plasmid#103995PurposePlasmid encoding DsRed-ExpressDepositorInsertDsRed-Express
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
TagsMBPExpressionBacterialMutationD832APromoterT7Available SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAK1.A
Plasmid#29460DepositorTypeEmpty backboneUseMycobacteria expressionExpressionBacterialPromoterHsp60Available SinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-SUMO-cSrc-SH2-AviTag
Plasmid#214230PurposeBacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selectionsDepositorInsertcSrc kinase SH2 domain (SRC Human)
Tags6xHis, AviTag, and SUMOExpressionBacterialPromoterT7Available SinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_cymR-PCymRC
Plasmid#204071PurposeType 0 promoter partDepositorInsertcymR repressor and PCymRC cumic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-150
Plasmid#118564PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbE8c-RFP
Plasmid#35269DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS30: pTarget(PmcCAST)
Plasmid#168163PurposeTarget plasmid containing PmcPSP1 and attachment site for PmcCAST.DepositorInsertsPAM for PmcCAST + PmcPSP1
tRNA-Val
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS7c-RFP
Plasmid#35320DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQacR-Q2
Plasmid#74690PurposeQacR-Q2 gateDepositorInsertPTac-RBSQ2-QacR-PQacR-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptet-sfGFP
Plasmid#139935PurposeaTc-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertTetR-Ptet-sfGFP
ExpressionBacterialAvailable SinceJuly 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKS2
Plasmid#24625DepositorInsert'tesA
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-mTmod3-WT
Plasmid#70753Purposemouse Tropomodulin3, WT for bacterial expressionDepositorAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
ExpressionBacterialPromoterPN25, Ptet, and PproKAvailable SinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_2
Plasmid#118226PurposeEmpty E. coli - B. burgdorferi shuttle vector; kanamycin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Csx30
Plasmid#197611PurposeFor protein purification of Csx30DepositorInsertSUMO_TwinStrep_Csx30
ExpressionBacterialAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
884 pGEX2T PTEN G129E
Plasmid#10747DepositorAvailable SinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAM232
Plasmid#226654PurposepET24b-p97-FLAGDepositorAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYH74
Plasmid#217382PurposepTrc99A-mNeonGreen-mcdB for bacterial expressionDepositorInsertmcdB
TagsNeonGreenExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28GG
Plasmid#104655PurposeLevel 2 cloning vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorTagsGST-mCherryExpressionBacterialAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_BGL2
Plasmid#101911PurposeExpresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.DepositorInsertβ-glucosidase
UseSynthetic BiologyExpressionBacterialPromoterT7+LacOAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-2xTS
Plasmid#109419PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and Tyrosine sulfation (TS) se…ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)Available SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAraTMDN
Plasmid#47515PurposeContains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assayDepositorInsertIntegrin alpha2B L980A TM-CYTO
ExpressionBacterialPromoterTrcAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK1007 (pSL6)
Plasmid#191002PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
ExpressionBacterialAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsHis tagExpressionBacterialPromoterT5 promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
ExpressionBacterialMutationC terminal domain onlyAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HSPA1LA
Plasmid#42429PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-R-GECO1.2
Plasmid#113937PurposeBacterial expression of red fluorescent Ca2+ sensor R-GECO1.2DepositorInsertR-GECO1.2
Tags6xHisExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE4n
Plasmid#17458Purposemodified pENTR vector for N-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGST and Thrombin siteExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKD279.8
Plasmid#173171PurposeEncodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporterDepositorInsertsSO_4388(REC)-PsdR(DBD) 137
sfGFP
ExpressionBacterialPromoterJ23108 and PpsdA110Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only