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pAKgfp1

Plasmid

#14076

Depositor

Attila Karsi

Publication

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Green fluorescent protein

Expression

Bacterial

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Publication

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pTP943

Plasmid

#104157

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fab specific nanobody TP886

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Publication

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSLTS

Plasmid

#59386

Purpose

Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

Depositor

Shelley Copley

Publication

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Type

Empty backbone

Use

Genome engineering

Expression

Bacterial

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-ATG8(416)/GFP-AUT7(416)

Plasmid

#49425

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

Depositor

Daniel Klionsky

Publication

Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pET His6 NusA TEV LIC cloning vector (1N)

Plasmid

#29657

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, NusA, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Publication

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pET28a-LIC

Plasmid

#26094

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

9 Articles

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pCHERRY3

Plasmid

#24659

Depositor

Tanya Parish

Publication

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSKBAD2

Plasmid

#15335

Depositor

Hideo Iwai

Publication

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein

Tags

GB1

Expression

Bacterial

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pMBP-AsCas12a

Plasmid

#113430

Purpose

Expresses 10xHis-MBP fused to AsCas12a

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

AsCas12a

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET30a-AlkB

Plasmid

#79050

Purpose

Express wild type AlkB protein in E. coli BL21(DE3)

Depositor

Tao Pan

Publication

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pET-28a-hPKM1

Plasmid

#44241

Depositor

Lewis Cantley, Matthew Vander Heiden

Publication

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M1 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGEM-Nanog

Plasmid

#16351

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens Nanog homeobox (NANOG Human)

Expression

Bacterial

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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