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Bacterial Expression

29,780 results

Showing: 321 - 340 of 29780 results

  8. pEVOL_PylRS(WT)-tRNA

    Plasmid
    #223512
    Purpose
    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD, rrnB, and glnS and proK
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    223512-plasmid-map-sequence-id-458970

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  9. pUC19-T7-3WJdB-T

    Plasmid
    #87308
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Three-way junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87308-plasmid-map-sequence-id-174739

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  11. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid
    #222101
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Bacteroidetes-bGSDM-TEV ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    TEV protease site replaces residues E242–G248
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222101-plasmid-map-sequence-id-440273

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  12. pET24a-LaM4-EGFP

    Plasmid
    #182641
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, EGFP, HA, His6, and T7
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182641-plasmid-map-sequence-id-358626

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  14. pUC19-P70a-3WJdB-T

    Plasmid
    #87311
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Three-way Junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87311-plasmid-map-sequence-id-174712

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  17. UHRF1

    Plasmid
    #28163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1 (UHRF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    tandem tudor domain
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pOPINCDE

    Plasmid
    #41135
    Depositor
    Ray Owens
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41135-plasmid-map-sequence-id-54729

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  19. CNN3A-c002

    Plasmid
    #175456
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-Y329.
    Depositor
    Opher Gileadi
    Insert
    CNN3 (CNN3 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175456-plasmid-map-sequence-id-353174

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Showing: 321 - 340 of 29780 results