31,059 results
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Plasmid#24127DepositorAvailable SinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only
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I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (940-953)
Plasmid#73746PurposeExpression of GFP-PP-RPN2 (940-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 940-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-ThermoPETase
Plasmid#218698PurposeEncodes codon optimized ThermoPETase enzyme for bacterial expressionDepositorInsertThermoPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SSA TLR (Sce)
Plasmid#45576PurposeCodes for the SSA-TLR with the target site for I-Sce I in a lentiviral backboneDepositorInserts5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm
UseLentiviralExpressionBacterial and MammalianMutationembedded I-Sce I TS from 163-185bp, truncated 25 …PromoterSFFVAvailable SinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH-MBP
Plasmid#101768PurposeGateway-compatible destination vector for bacterial expression of N-terminal MBP-tag fusion proteinDepositorTypeEmpty backboneTagsMBPExpressionBacterialAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA2c-RFP
Plasmid#35326DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840AAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG372
Plasmid#115602Purposesurface-displayed Nb1; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
ExpressionBacterialAvailable SinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUA66 theta GFP
Plasmid#105607PurposeExpresses GFP with no promoterDepositorInsertGFP
ExpressionBacterialPromoterNo PromoterAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2B
Plasmid#15781DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
Tags6xHisExpressionBacterialPromoterPtacAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
HMBP-3C-STAR.66-285
Plasmid#100094Purposeexpresses MBP-tagged STARD1 protein domain (residues 66-285)DepositorAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
Tags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
KDM4D
Plasmid#73247PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertKDM4D (KDM4D Human)
TagsHis-6-TEVExpressionBacterialMutationpfam02375:JmjN 18-64, pfam02373:JmjC 182-295PromoterT7Available SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI
Plasmid#117006PurposeExpresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only