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32,771 results

Showing: 541 - 570 of 32771 results

  1. pEM-Cas9HF1

    Plasmid
    #89961
    Purpose
    Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.
    Depositor
    Michael Lynch
    Article
    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021
    Insert
    Cas9HF1
    Expression
    Bacterial
    Mutation
    N497A/R661A/Q695A/Q926A
    Promoter
    pTet
    Available Since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    89961-plasmid-map-sequence-id-181339

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  3. pET24a-LaM4-EGFP

    Plasmid
    #182641
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    Tags
    BAP, EGFP, HA, His6, and T7
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182641-plasmid-map-sequence-id-358626

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  7. pGBW-m4092677

    Plasmid
    #153883
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    wt;handful of SNPs as watermarks
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153883-plasmid-map-sequence-id-307322

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  8. pGBW-m4092694

    Plasmid
    #153907
    Purpose
    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153907-plasmid-map-sequence-id-307331

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  9. pGBW-m4092709

    Plasmid
    #153859
    Purpose
    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Bacterial
    Mutation
    Nearly native encoding;handful SNPs as watermarks…
    Promoter
    Promoter | t7 consensus from NEB, G is +1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153859-plasmid-map-sequence-id-301594

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  13. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Available Since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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  14. pATB_151

    Plasmid
    #226038
    Purpose
    Plasmid encodes an AT-FP with YeeJ signal peptide, epitope linker, YeeJ translocation unit enabling the display of a passenger of interest at the bacterial cell surface
    Depositor
    Joachim Jose
    Article
    Furtmann et al Commun Biol. 2026 May 21;9(1):694. doi:
    Insert
    default passenger (=insert) is cel5
    Tags
    His and PEYFK-myc
    Expression
    Bacterial
    Promoter
    Arabinose inducible (araBAD)
    Available Since
    May 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    226038-plasmid-map-sequence-id-477119

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  20. pSUB11

    Plasmid
    #169699
    Purpose
    Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    3xFLAG tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169699-plasmid-map-sequence-id-336760

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  25. pEVOL-pylT-N346A/C348A

    Plasmid
    #127411
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers
    Depositor
    Wenshe Liu
    Article
    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A
    Promoter
    araBAD
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127411-plasmid-map-sequence-id-250214

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  28. GCN5L2

    Plasmid
    #25482
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GCN5L2 (KAT2A Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. HDAC6 (3GV4)

    Plasmid
    #25297
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6_42 (HDAC6 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 1109-1215
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 541 - 570 of 32771 results