33,906 results
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Plasmid#118225PurposeEmpty E. coli - B. burgdorferi shuttle vector, gentamicin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBSV2B
Plasmid#118228PurposeEmpty E. coli - B. burgdorferi shuttle vector, blasticidin S resistantDepositorTypeEmpty backboneExpressionBacterialAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-GH023 - NanoLuc
Plasmid#117727PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023 upstream of the NanoLuc reporterDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
TagsC terminal TEV GFP octa-His affinity tagExpressionBacterialPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE-Tn7-167
Plasmid#118579PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorExpressionBacterialAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
TagsSUMOExpressionBacterialAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG320
Plasmid#115597Purposesurface-displayed Nb2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKEW189-MBP-TEV-AcrVA1
Plasmid#115660PurposeExpresses AcrVA1 in bacteria via T7 RNAP, for purificationDepositorInsertAcrVA1
TagsMBP-TEVExpressionBacterialPromoterT7 RNAPAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRETDepositorInsertH-Luc
UseLuciferaseExpressionBacterialPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
TagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialPromoterT7lacAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-APLO
Plasmid#112805Purposegateway cloning destination vector to express LexAop2 driven transgene in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE80L SpyTag-ELP-SpyTag
Plasmid#112634PurposeTwo SpyTags linked by an elastin-like polypeptide for hydrogel formationDepositorInsertSpyTag-ELP-SpyTag
TagsHis6ExpressionBacterialMutationcentral cysteinePromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL1-YPet
Plasmid#102633PurposeIn vitro expression of YPet from the T7 promoterDepositorInsertYPet
ExpressionBacterialPromoterT7Available sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK552
Plasmid#110618PurposeBTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.DepositorInsertGFP
ExpressionBacterialAvailable sinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2
Plasmid#111259Purposemini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmTurquoise2
ExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTn7CdrA-gfp(ASV)C
Plasmid#111617PurposeFluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.DepositorInsertPcdrA-RBSII-gfp(ASV)-T0-T1
ExpressionBacterialAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SB_loop
Plasmid#110103PurposepBXPHM3 with scaffold Sybody of the loop libraryDepositorInsertscaffold Sybody of the loop library
TagsHisExpressionBacterialPromoterPBADAvailable sinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by