29,747 results
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Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorInsertrpsA (rpsA Escherichia coli)
UseTagsHis tagExpressionBacterialMutationPromoterp-T5 LacAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
UseTagsN-terminal His tag with thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(cAMP-1.6)/pRSETB
Plasmid#53591PurposeNano-lantern-based luminescent cAMP indicatorDepositorInsertNano-lantern-based luminescent cAMP indicator
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-Caspase-4_enzymatic-domain
Plasmid#183381PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-4 (AA94-377) (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-29b-3xGluGlu-c-jun-His6
Plasmid#129227PurposeEncodes 3xGluGlu tagged human c-Jun for purification by nickel affinityDepositorInsertcJun (JUN Human)
UseTags6xHistidineExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJV0047
Plasmid#205139PurposeExpression of 6xHis-EGFP-2xPX in bacteriaDepositorInserttandem 2xPX domain of SnxA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
UseTagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-LOV2
Plasmid#185792PurposeEncodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linkerDepositorInsertRBP-LOV2
UseTags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1
Plasmid#120361PurposeProduce human recombinant TGFB1 in DE3 cellsDepositorInsertTransforming Growth Factor-β1 (TGFB1 Human, Synthetic)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-IG-mSA
Plasmid#80706PurposeExpresses monomeric streptavidin (mSA) in bacterial cellsDepositorInsertMonomeric streptavidin
UseTags10xHis tag and TEV siteExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease
Plasmid#228637PurposeBacterial expression of the Coxsackievirus 16 3C proteaseDepositorInsertCoxsackievirus 16 3C protease
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNMTMA_MBP-Cav1-His6
Plasmid#223700PurposeTo produce standard caveospheres (without the Z-domain), Parton Lab clone LDBDepositorArticleInsertMBP-Cav1-His6
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
hsp70: mKate2-DN Kif5a
Plasmid#105972Purposeheat shock inducible transgenesisDepositorInsertKif5a (kif5aa Zebrafish)
UseTagsmKate2ExpressionBacterialMutationtruncation of aa416-1033PromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-His
Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneUseTags10xHis and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7Available sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only