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pVRc03_1198
Plasmid#49730DepositorInsertAS03_1198
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc22_4450
Plasmid#49741DepositorInsertAS22_4450
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM5087
Plasmid#85123PurposePtrc-YFP subcloned from pAM5080 into NSII-Km vector.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRD7 (BRD7A-c005)
Plasmid#98245PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRD7A (BRD7 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00439:Bromodomain 136-223PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOP3SU
Plasmid#112599PurposeUsed for expression of protein in E. coli as N-terminal Sumo tag fusionsDepositorTypeEmpty backboneUseTagsSumoExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcF-1
Plasmid#131023PurposeSingle spacer array with spacer #1 for FnCas12a.DepositorInsertsingle spacer array for FnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23119Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YWHAE_14-3-3
Plasmid#109942PurposeProtein expression and purification of YWHAE_14-3-3DepositorInsertYWHAE_14-3-3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
40 pCOLI_K_MBP
Plasmid#160183PurposeN-term MBP 3C cleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-hnRNP-F
Plasmid#23021DepositorInserthnRNP-F (HNRNPF Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBT3035
Plasmid#122543PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG004
Plasmid#113979PurposeVector construction module. Level 1 golden gate cloning site module with LacZ reporterDepositorInsertLevel 1 golden gate cloning site
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSP577
Plasmid#98801PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
UseTagsExpressionBacterialMutationI138T, N171D, N176TPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mEGFPBb
Plasmid#118234PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFPDepositorInsertflagellin promoter driven mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PC4_3spc34h
Plasmid#70700PurposeExpresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34DepositorInsertUseTagshis-tag on C-terminus of spc34ExpressionBacterialMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_MDM2_SWIB
Plasmid#178481PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertMDM2_SWIB (MDM2 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Yes-SH2
Plasmid#46532DepositorInsertYes (YES1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 152-261PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL005
Plasmid#92327PurposeExpress FtsZ tagged with mEos3.2 in E coliDepositorInsertFtsZ
UseTagsmEos3.2ExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQStrep2-CRYM
Plasmid#31293DepositorInsertcrystallin, mu (CRYM Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-RFP
Plasmid#35280DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-hu FceRI beta
Plasmid#8366DepositorInserthuman Fc epsilon RI beta cDNA (MS4A2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSPAsp-hp
Plasmid#48122PurposeShuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoterDepositorInsertpredicted optimal signal peptide for Bacillus megaterium protein secretion
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pCoofy16
Plasmid#43988DepositorTypeEmpty backboneUseTagsHis10-NusAExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin
Plasmid#186137PurposeFor expression of recombinant beta1 integrin cytosolic domain in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and untranslated His6 after stop codonExpressionBacterialMutationPromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG8H-EWS_1-264
Plasmid#180467PurposeExpresses the construct His8-EWS_1-264 with a TEV cleavage site for the removal of the His8 tagDepositorInsertEWSR1 (EWSR1 Human)
UseTagsHis8ExpressionBacterialMutationdeleted 265-656PromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG06-pLac-NRI
Plasmid#102463PurposeExpresses NRI protein in E. coli. The expression is controlled by a pLac promoter.DepositorArticleInsertNRI (ntrC )
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMSR:MSMEG_0030:Dendra
Plasmid#159040PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0030
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold TDP43 G294A
Plasmid#27452DepositorInsertTDP-43 (TARDBP Human)
UseTagsFactor Xa and His6ExpressionBacterialMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 1039-1176
Plasmid#53351Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 1039-1176PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 681-880
Plasmid#53349Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 681-880PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GST-PRDM16 455-680
Plasmid#53348Purposebacterial expression of mouse PRDM16 fragmentDepositorInsertPRDM16 (Prdm16 Mouse)
UseTagsGSTExpressionBacterialMutationaa 455-680PromotertacAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2
Plasmid#116965PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
4P7A
Plasmid#61954PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMLH1:JMC01L-F09:C205690 (MLH1 Human)
UseTags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Reb_82 aka PAS623
Plasmid#71228PurposeReb_1 with RebB(S82P, D88G)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebB(S82P, D88G), RebA (G53A)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_67 aka PAS627
Plasmid#71232PurposeReb_1 with RebA(S88A)DepositorInsertReb locus
UseTagsnoneExpressionBacterialMutationRebA(G53A, S88A)PromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL22Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
prCAG-RBS"m1"-sfGFP
Plasmid#191400PurposeExpression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)DepositorInsert47xCAG RBS"m1"-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLtet0-1Available sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEV-Gly-RadA C46
Plasmid#128736PurposeExpresses PhoRadA intein split at residue 46 from the C-terminalDepositorInsertRadA
UseTagsHisx6-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAT4
Plasmid#112585PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusionsDepositorTypeEmpty backboneUseTags6HisExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-VCPIP (OTU, aa 25-561)
Plasmid#61583PurposeExpresses human VCPIP (OTU domain) in E. coli.DepositorInsertVCPIP (VCPIP1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-24 and aa 562-1222.PromoterT7Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFCiR5
Plasmid#149479PurposeBroad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
KaiC
Plasmid#41884DepositorInsertKai C
UseTagshistadineExpressionBacterialMutationPromotert7Available sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only