32,672 results
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Plasmid#64911PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in E. ColiDepositorInserthsGFP
TagsHis TagExpressionBacterialMutationChromophore tyrosine of the protein mutated to TA…PromoteraraBADAvailable SinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PDAP1
Plasmid#31586DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)
Plasmid#141345PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).DepositorInsertGB1His6-TEV-ALIX(800-868)
TagsGB1-His6-TEVExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOXdel
Plasmid#186785PurposeC-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
TagsHis tagExpressionBacterialMutationdeleted amino acids 503-512Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_TetR/pTet-Tnpase (carbR)
Plasmid#213911PurposeATc-inducible himar1C9 transposase expression - carb/amp resistanceDepositorInsertTetR/ptet ATc-inducible himar1C9 transposase
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn[sfGFP]
Plasmid#213902PurposeMariner transposon expressing sfGFP with kan selection casetteDepositorInsertmariner transposon with kan resistance and superfolder GFP
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 141-465
Plasmid#45971DepositorInsertParkin 141-465 (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3991 pET ubcH9
Plasmid#8651DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
SRpHi2
Plasmid#102542PurposeBacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EGFP
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbO
Plasmid#114153PurposeExpresses ClbODepositorInsertclbO
Tags6xHisExpressionBacterialAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T2 Parkin RBR
Plasmid#31255DepositorAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
HTRA1_PDZ_1
Plasmid#103912PurposeProtein expression and purification of HTRA1 PDZ domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS mouse c-Myc
Plasmid#14972DepositorAvailable SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJB01
Plasmid#111957PurposeExpresses His10-RFP-Halo; construct generated for pull-down and localization experimentsDepositorInsertRFP
TagsHaloTagExpressionBacterialPromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-GST-eIF4G1-His6, eIF4E
Plasmid#37232DepositorTagsGST and His6ExpressionBacterialPromoterT7Available SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
rpoS200-RFP
Plasmid#63840PurposeThis plasmid is designed as a bacterial stationary phase sensorDepositorInsertrpoS, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
ExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBZcsx27
Plasmid#89900PurposeExpresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx27
ExpressionBacterialPromoterLacAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS1398
Plasmid#205971PurposeMWEE01000013 Cas5-HNH crRNA expression in pCOLADuet-1DepositorInsertCas5-HNH crRNA
ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2A
Plasmid#145343PurposeCell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFPDepositorInsertZIKV NS2A
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceFeb. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeExpressionBacterial and MammalianPromoterNoneAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
MAP1LC3A
Plasmid#25185PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
TOPOII p48 (DM#261)
Plasmid#15026DepositorInsertp48 (Ptf1a Mouse)
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO8Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
NUMBLA
Plasmid#42420PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDB24
Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneTags10-His and Smt3ExpressionBacterial and YeastPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originExpressionBacterialAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZH514
Plasmid#102667PurposeConstitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT3011
Plasmid#122539PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
ATAD2
Plasmid#38916PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBHM2642
Plasmid#229526PurposeCarries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2645
Plasmid#229529PurposeCarries mIAA7_opt tag, empty sgRNA, and NATR markerDepositorInsertmIAA7_opt - empty sgRNA - NATR
UseCRISPRTags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2646
Plasmid#229530PurposeCarries mAID_opt tag, empty sgRNA, and NATR markerDepositorInsertmAID_opt - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2647
Plasmid#229989PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2648
Plasmid#229990PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2649
Plasmid#229991PurposeCarries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mIAA7_optExpressionBacterial and YeastMutationF74GAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2650
Plasmid#229992PurposeCarries AID* tag, empty sgRNA, and HYGR markerDepositorInsertAID* - empty sgRNA - HYGR
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2651
Plasmid#229993PurposeCarries AID* tag, empty sgRNA, and NEOR markerDepositorInsertAID* - empty sgRNA - NEOR
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2652
Plasmid#229994PurposeCarries AID* tag, empty sgRNA, and amdS markerDepositorInsertAID* - empty sgRNA - amdS
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpyB
Plasmid#220186PurposesgRNA expression vector - pBG35 promoterDepositorInsertpBG35 promoter
UseCRISPRExpressionBacterialPromoterpBG35Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MERTK_HUMAN_D0
Plasmid#79705PurposeThis plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6
Plasmid#180313Purposebacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6DepositorTagsHis6-TEV and sfCherry2ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCPP5537
Plasmid#128730PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAO1
ExpressionBacterialAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
TagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CH10-GG
Plasmid#215807PurposeCloning of target gene with C terminal non cleavable His10 tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits