32,684 results
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Plasmid#159373PurposeExpresses STEPtag in E. coliDepositorInsertSTEPtag
Tags6x His tagExpressionBacterialPromoterT7Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T NHE-RF
Plasmid#11636DepositorAvailable SinceMay 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKNR5
Plasmid#149466PurposeBroad-Host range expression vector, RK2 and pUC origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertNahR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYC.GFP1Amb
Plasmid#102792PurposeExpresses GFP with a premature stop codonDepositorInsertGreen Fluorescent Protein
ExpressionBacterialMutationY39StopAvailable SinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGB
Plasmid#139932PurposeM. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)DepositorInsertsfGFP
ExpressionBacterialPromoterPJ23101Available SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-SARS-CoV-2-Nucleocapsid
Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6268 pDEST-59-His-Trx-HPV16E6
Plasmid#24126DepositorAvailable SinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PEPD
Plasmid#31328DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-136
Plasmid#118522PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN-01*
Plasmid#80423PurposeFor expression of EPN-01* in E. coliDepositorInsertEPN-01*
TagsMycExpressionBacterialAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7C-DNAJB6
Plasmid#177657PurposeRecombinant protein synthesis of human DNAJB6DepositorAvailable SinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5588
Plasmid#128708PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopD1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5053
Plasmid#128707PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopC1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5530
Plasmid#128705PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopA1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23a RanGAP
Plasmid#13387DepositorInsertRanGAP (RANGAP1 Human)
ExpressionBacterialAvailable SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Bov.hsc70.RSET.FL.wt(NarI)
Plasmid#12532DepositorInsertbovine 70 kDa heat shock cognate protein full length wild type
ExpressionBacterialMutationwild typeAvailable SinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAB143
Plasmid#101679PurposeLAA-tagged mCherry under T7 promoter controlDepositorInsertLAA-tagged mCherry under T7 promoter control
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12DKR-J
Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Hyg)
Plasmid#110082PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK210
Plasmid#73325PurposeProduces Janthinobacterium lividum violacein biosynthesis protein VioDDepositorInsertviolacein biosynthesis protein VioD
ExpressionBacterialPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCH6 - pBait-ChiX
Plasmid#174662Purposearabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertChiX
Tags1xMS2 hairpinExpressionBacterialPromoterpBadAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRExpressionBacterialPromoterPj23119Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLitR-L1
Plasmid#74683PurposeLitR-L1 gateDepositorInsertPTac-RBSL1-LitR-PLitR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRset-OppA
Plasmid#207869PurposeBacterial expression of poly-his-tagged L. lactic OppADepositorInsertOppA
Tags6xHisExpressionBacterialPromoterT7Available SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Sam68
Plasmid#17687DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
BLVRA
Plasmid#38856PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #1
Plasmid#104208PurposeBacterial expression of WW domain #1 from NEDD4DepositorInsertNEDD4 WW domain #1 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
RAB14
Plasmid#25519PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-HisD-miRFP
Plasmid#108410PurposeMonomeric near-infrared fluorescent protein miRFP in bacterial expression plasmidDepositorInsertmiRFP
ExpressionBacterialPromoteraraBAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_TELO2_dTAG
Plasmid#224380PurposeGenerates TELO2 dTAG N terminal knock-in cell linesDepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19_GNB1L_dTAG
Plasmid#224378PurposeGenerates GNB1L dTAG C terminal knock-in cell linesDepositorAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn
Plasmid#206965PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGTDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CACNB1_Guanylate-kin
Plasmid#110036PurposeProtein expression and purification of CACNB1_Guanylate-kinDepositorInsertCACNB1_Guanylate-kin (CACNB1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
130_pSKB3-LUMP-P.leioghnati
Plasmid#65894PurposeBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetimeDepositorInsertLUMP
ExpressionBacterialAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Bmf
Plasmid#24264DepositorAvailable SinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKB233
Plasmid#170040PurposeEncodes PvirK-mNeonGreen reporterDepositorArticleInsertmNeonGreen
UseSynthetic BiologyExpressionBacterialPromoterPvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBADM11-HMGR
Plasmid#108946Purposeheterologous expression of HMGR of S. solfataricus in E. coliDepositorInsertHMG-CoA reductase (HMGR)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter
Plasmid#65007PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning siteDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoternoneAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSARSF53-110
Plasmid#61813PurposeTvoVMAN11 inteinDepositorInsertTvoVMAN11 intein
TagsH6-Smt3ExpressionBacterialPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-98
Plasmid#61827PurposeTvoVMAdN11 inteinDepositorInsertTvoVMAdN11 intein
TagsGB1-H6ExpressionBacterialPromoterpBADAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Hyg)
Plasmid#110077PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Z-UBQLN2-7
Plasmid#166563PurposescFv of a human scaffold targeting Ubiquilin-2. Antigen coverage aa 1-624 of 624 aa, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP325
Plasmid#159596PurposeExpress E.coli TonB proteinDepositorInserttonB
ExpressionBacterialMutationnoneAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR PKAKTRn-Clover
Plasmid#177199PurposeGateway entry vector encoding PKAKTRnew-CloverDepositorInsertPKAKTRnew-mClover
ExpressionBacterialAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only