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Plasmid#22380DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHis and mycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w5-12-C81S
Plasmid#18043DepositorInsertHhaI C81S mutant
UseTagsExpressionBacterialMutationSimply used as an insert between Kpn1 and Xba1PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src42a
Plasmid#126673PurposeExpression of GST-Src42a for purification from E. coliDepositorInsertSrc42a
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72110-BBa_J72094
Plasmid#40778DepositorInsertAraC-pBad + coi
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046942
Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p005kanRFP
Plasmid#58533PurposeRFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.DepositorInsertRed fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5 between two lac operatorsAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRFP1*
Plasmid#104000PurposePlasmid encoding mRFP1*DepositorInsertmRFP1*
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLAVIKEF...T…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKEN GFP mut3
Plasmid#20410DepositorInsertGFP
UseTagsExpressionBacterialMutationS65G S72APromoterAvailable sinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(pos36GFP)-Cys
Plasmid#71757PurposeAurein1.2_+36GFP-Cys expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-Cys
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC010 LshCas13a locus with nontargeting spacer
Plasmid#91901PurposeLshCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLshCas13a locus with non-targeting spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSPYngK+-hp
Plasmid#48121PurposeShuttle vector E. coli/B.meg.; carrying the codon optimized sequence of the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertcodon optimized signal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKB239
Plasmid#170034PurposeEncodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA2a-RFP
Plasmid#35325DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTetAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA2_RPA-C
Plasmid#109795PurposeProtein expression and purification of RPA2_RPA-CDepositorInsertRPA2_RPA-C (RPA2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmMaple2-CAM
Plasmid#101149PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple2
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA2-RFP
Plasmid#62248Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA2Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Let7-SunTag
Plasmid#196928PurposeLet7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Let7R-SunTag
Plasmid#196929PurposeReverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and MS2 stem-loops in the SunTag reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2
Plasmid#134288PurposeExpresses GK5 (isoform 2) in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationmouse Gk5 isoform 2; 534 amino acidsPromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVZ_PcoaT_SQE1_LUP1
Plasmid#103857PurposeHeterologous, cobalt-inducible expression of SQE1 and LUP1 from A. thaliana in Synechocystis sp. PCC6803DepositorInsertlupeol synthase 1 (LUP1 Mustard Weed)
UseSynthetic BiologyTagsExpressionBacterialMutationinserted genes are codon optimized. 2nd amino aci…PromoterPcoaT (from Synechocystis sp. PCC 6803)Available sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pGEX p85a(C)-SH2
Plasmid#46463DepositorInsertp85a(C) (PIK3R1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 612-724PromoterTacAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
TOPO CCSP
Plasmid#68453PurposeTOPO construct expressing CCSP for generation of GMAP-compatible promoterDepositorInsertClara Cell Secretory Protein Promoter
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbP
Plasmid#48244PurposeUntagged, full-length protein for in vivo peptidase assaysDepositorInsertClbP
UseTagsNoneExpressionBacterialMutationPromoterT7Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEB1-comTurquoise2
Plasmid#104018PurposePlasmid encoding comTurquoise2DepositorInsertcomTurquoise2
UseLow copyTagsExpressionBacterialMutationSame protein sequence as mTurquoise2 (codon optim…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Drp1(A395D)-StrepII
Plasmid#174430PurposeExpresses human Drp1 Isoform 3 with A395D mutation in bacteriaDepositorInsertDrp1
UseTagsStrepIIExpressionBacterialMutationA395DPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drp1-StrepII
Plasmid#174428PurposeExpresses human Drp1 Isoform 3 in bacteriaDepositorInsertDrp1
UseTagsStrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH016
Plasmid#80400PurposeNo anti-scaffold control with 3xFLAG tags for Western blots. Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG).DepositorUseSynthetic BiologyTagsC-terminal 3xFLAG tag and C-terminal LZx domainExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOSV808
Plasmid#126601Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC4 Frk-SH2
Plasmid#46428DepositorInsertFrk (FRK Human)
UseTagsAviTag, GST, and Thrombin siteExpressionBacterialMutationcontains amino acids 108-213PromoterTrcAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#22869DepositorInsertpCph8
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 517 amino acids of Cph1 and the last 229 am…PromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
UseTags6His and mycExpressionBacterialMutationPromoterpBADAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL80
Plasmid#26257DepositorInsertGAL80 (GAL80 Budding Yeast)
UseDrosophila transgenesisTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
ISG15 C-term. Domain intein/chitin binding domain (79-156)
Plasmid#110760PurposeBacterial expression of ISG15 C-term.DepositorInsertISG15 (ISG15 Human)
UseTagsintein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sup35 ScNM A230C His7
Plasmid#15598DepositorInsertSup35 NM (SUP35 Budding Yeast)
UseTags7xHisExpressionBacterialMutationA230C. Alanine 230 mutated to cysteine.PromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDHK29
Plasmid#80783PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-attL5-CRY2-mCh-attL2
Plasmid#160438PurposeEntry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombinationDepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP161
Plasmid#189536PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFPDepositorInsertsRNase III
gUTR-161-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HS-Utrophin-GFP
Plasmid#105952Purposeheat shock inducible transgenesisDepositorInsertUtrophin (Utrn Mouse)
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
rsGFP1-pBAD
Plasmid#54851PurposeLocalization: Protein Expression Vector , Excitation: 493, Emission: 510DepositorTypeEmpty backboneUseTags6xHis and rsGFP1ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P-Cdh1wt (570)
Plasmid#39877DepositorInsertFZR1 (FZR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
C2-66 pComb3xss anti-cFos nanobody
Plasmid#145833PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C2-66 (FOS L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3034 GST-HPV-11 E6
Plasmid#10848DepositorInsertHPV11 E6
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDZ207 (loxP KAN loxP 24xPP7SL MDN1)
Plasmid#35191DepositorInsertloxP KAN loxP 24xPP7SL MDN1
UseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET23b-Casp9 C287A-His
Plasmid#11830DepositorInsertCaspase 9 catalytic mutant (CASP9 Human)
UseTags6xHisExpressionBacterialMutationC287APromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
UseTagsExpressionBacterialMutationSUMO tag fused to N-terminus of PylBPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only