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Showing: 10751 - 10800 of 29646 results

  3. pHH0103 NEDD4L WW domain #3

    Plasmid
    #104206
    Purpose
    Bacterial expression of WW domain #3 from NEDD4L
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    NEDD4L WW domain #3 (NEDD4L Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104206-plasmid-map-sequence-id-202575

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  4. KAT2A

    Plasmid
    #38914
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KAT2A (KAT2A Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

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  9. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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  21. pKS011

    Plasmid
    #65464
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
    Depositor
    Keith Tyo
    Article
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    MBP-Ntag-mCherry
    Use
    Tags
    FLAG and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    65464-plasmid-map-sequence-id-116873

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  24. pSecEffector

    Plasmid
    #172891
    Purpose
    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901
    Depositor
    Urartu Seker
    Article
    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696
    Inserts
    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ProD, nTETO, and pBAD /AraC
    Available since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172891-plasmid-map-sequence-id-368224

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  27. p404MET25

    Plasmid
    #17420
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    MET25 promoter is between SacI and XbaI restricti…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17420-plasmid-map-sequence-id-8595

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  29. ZP11 Eng2

    Plasmid
    #16930
    Depositor
    Randall Moon
    Insert
    eng2 (eng2a Zebrafish)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2008
    Availability
    Academic Institutions and Nonprofits only

  30. 2P0E

    Plasmid
    #60109
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C9orf95:C9orf95_dml-X01:C201158 (NMRK1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. JMJD6

    Plasmid
    #39100
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD6 (JMJD6 Human)
    Use
    Tags
    His6-Thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. FDPS

    Plasmid
    #39131
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FDPS (FDPS Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  46. pelB-MBP-BB0323-ss

    Plasmid
    #137067
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0323: lacks the BB0323 signal peptide
    Promoter
    T7lac
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137067-plasmid-map-sequence-id-291957

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  48. PTPN18

    Plasmid
    #39168
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN18 (PTPN18 Human)
    Use
    Tags
    GST-PreScission
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10751 - 10800 of 29646 results