32,811 results
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Plasmid#199437Purposeexpresses BAmGreen1.3 in bacterial cellsDepositorInsertBAMGreen1.3
Tagshis tagExpressionBacterialPromoterT7Available SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PDCD6_EF-hand
Plasmid#110009PurposeProtein expression and purification of PDCD6_EF-handDepositorInsertPDCD6_EF-hand (PDCD6 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
TagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-tdTomato-hphMX6
Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
TagstdTomatoExpressionBacterial and YeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AK3L1
Plasmid#25541PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBA562
Plasmid#186237PurposepTet-RfxCas13d crRNA entry vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG017 (AVAST type 1)
Plasmid#157895PurposeExpresses the AVAST type 1 defense systemDepositorInsertAVAST type 1 (STAND ATPase with N-terminal serine protease)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCL1333
Plasmid#217966PurposeBacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.DepositorAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
TagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialPromoterT7Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_1
Plasmid#103909PurposeProtein expression and purification of ZO1 PDZ1 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-RFP
Plasmid#35281DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMyCA
Plasmid#84268PurposeA versatile vector for recombinant mycobacterial protein productionDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAceteamidaseAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-Rac1
Plasmid#92039PurposeExpresses mEos3-Rac1 fusion proteinDepositorAvailable SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 Wt
Plasmid#29545DepositorAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
MiCy-pBAD
Plasmid#54565PurposeLocalization: Protein Expression Vector , Excitation: 472, Emission: 495DepositorTypeEmpty backboneTags6xHis and MiCyExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
ExpressionBacterialPromoteraraBAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ub-GyrA
Plasmid#128737PurposeExpresses Ub fused to GyrA intein at the C-terminalDepositorInsertUBC
TagsMxe GyrAExpressionBacterialPromoterT7Available SinceSept. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
TagsHisx6ExpressionBacterialAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-FRB-PafA
Plasmid#113404PurposeExpresses FRB-PafA in bacteriaDepositorInsertFRB-PafA
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB167
Plasmid#101672PurposeOpto-T7RNAP*(563), equal expr.DepositorInsertOpto-T7RNAP*(563), equal expr.
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only