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32,812 results

Showing: 6601 - 6630 of 32812 results

  4. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  5. pRaGE Pyl TAG GFP Y35TAG

    Plasmid
    #160041
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5
    Inserts
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    deGFP
    Use
    Synthetic Biology
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed tyrosine 35 to TAG stop-codon (Site numbe…
    Promoter
    P70b promoter and T500 terminator, Vibrio natrieg…
    Available Since
    May 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160041-plasmid-map-sequence-id-332393

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  8. PTK2BA

    Plasmid
    #42401
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTK2BA (PTK2B Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pGGD_RT_SRDX

    Plasmid
    #136976
    Purpose
    Goldengate/Greengate module EAR-repression domain
    Depositor
    Klaus Theres
    Insert
    SRDX
    Expression
    Bacterial
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136976-plasmid-map-sequence-id-272093

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  11. pBJ1

    Plasmid
    #167130
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Frr promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167130-plasmid-map-sequence-id-330432

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  13. pELxG4

    Plasmid
    #172570
    Purpose
    Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-GFPmut3
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLuxB
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172570-plasmid-map-sequence-id-347356

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  14. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid
    #74102
    Purpose
    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
    Depositor
    Ben Szaro
    Article
    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:
    Inserts
    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    Expression
    Bacterial
    Available Since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74102-plasmid-map-sequence-id-148113

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  17. pGal4x5-E4T-G210

    Plasmid
    #171077
    Purpose
    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Insert
    Gal4-E4-Gless-cassette
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171077-plasmid-map-sequence-id-335554

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  20. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  22. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Tags
    6His-TEV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

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  23. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  28. METTL1

    Plasmid
    #25264
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL1_07 (METTL1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pDS170-enNTS1

    Plasmid
    #199152
    Purpose
    JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)
    Depositor
    Daniel Scott
    Article
    Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:
    Insert
    enNTS1 (Ntsr1 Rat)
    Tags
    10xHis tag, Avi tag, MBP, and muGFP
    Expression
    Bacterial
    Mutation
    V208M, C273S, C391S and C393S (over NTS1-B5)
    Promoter
    Lac/T5
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199152-plasmid-map-sequence-id-393468

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Showing: 6601 - 6630 of 32812 results