Skip to main content
Addgene
Showing: 11001 - 11050 of 29646 results

  1. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

    Enlarge

  7. L-YARS2-9

    Plasmid
    #166533
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase 2, mitochondrial. Antigen coverage aa 31-477 of 477
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166533-plasmid-map-sequence-id-329205

    Enlarge

  8. pTH849-gst-optec

    Plasmid
    #89590
    Purpose
    Plasmid for bacterial expression of His-tagged GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89590-plasmid-map-sequence-id-206038

    Enlarge

  10. pNIC28-BIRC7

    Plasmid
    #196363
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC7/Livin
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196363-plasmid-map-sequence-id-386127

    Enlarge

  11. pNIC28-BIRC3

    Plasmid
    #196366
    Purpose
    protein expression of the His-tagged full length protein
    Depositor
    Stefan Knapp
    Insert
    BIRC3/cIAP2
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196366-plasmid-map-sequence-id-386071

    Enlarge

  14. SMYD3

    Plasmid
    #25318
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3_26 (SMYD3 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pGEM-mcpGFP

    Plasmid
    #47442
    Purpose
    contains modified circularly permutated EGFP (mcpGFP)
    Depositor
    Wolf Frommer
    Article
    De Michele et al eLife. 2013 Jul 2;2:e00800.
    Insert
    mcpGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    M154K, V164A, S176G, D181Y, T204V, A207K, V190I
    Promoter
    Available since
    Sept. 16, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. UBC64

    Plasmid
    #25143
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc64.248.414 (UBE2Q1 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains UBC domain; residues 248-414
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pEasyG3_hph

    Plasmid
    #184919
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184919-plasmid-map-sequence-id-368411

    Enlarge

  45. CDKL1_HUMAN_D0

    Plasmid
    #79699
    Purpose
    This plasmid encodes the kinase domain of CDKL1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    Markus Seeliger, John Chodera, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    CDKL1 (CDKL1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  46. MAP3K3

    Plasmid
    #38937
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAP3K3 (MAPK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  49. pGBW-m4046789

    Plasmid
    #145660
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145660-plasmid-map-sequence-id-298333

    Enlarge

Showing: 11001 - 11050 of 29646 results