31,059 results
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Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-146
Plasmid#118530PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FabF (KASII) pet28 N-terminal His Tag
Plasmid#75017PurposeExpresses the ketosynthase FabF/KASII from the E. coli fatty acid synthaseDepositorAvailable SinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-mPlum
Plasmid#158062PurposeDonor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertmonomeric plum fluoresecent protein
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Reb_82 aka PAS623
Plasmid#71228PurposeReb_1 with RebB(S82P, D88G)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebB(S82P, D88G), RebA (G53A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_67 aka PAS627
Plasmid#71232PurposeReb_1 with RebA(S88A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, S88A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPro18-cm
Plasmid#17807DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
ME1
Plasmid#38857PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA11_G-alpha
Plasmid#109911PurposeProtein expression and purification of GNA11_G-alphaDepositorInsertGNA11_G-alpha (GNA11 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE502B
Plasmid#128971PurposeBroad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
ExpressionBacterialMutationE32KPromoterxyl-PmAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-RdgBbeta-splice variant 1
Plasmid#58276Purposebacterial expression of human RdgBbeta splice variant 1DepositorAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATG --> TGTTGGAGAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RAC1 (RAC1A-c007)
Plasmid#110257PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRD421
Plasmid#163112PurposeProduction of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysSDepositorInsertHis-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
TagsHis-tag with TEV-cleavable linkerExpressionBacterialPromoterT7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL658
Plasmid#167837PurposeEncodes OptoLAC1B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (LAA)ExpressionBacterialAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL659
Plasmid#167838PurposeEncodes OptoLAC2B circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO mCER-Prx2
Plasmid#158226PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)DepositorInsertmCER-Prx2
ExpressionBacterialAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nkx6.2
Plasmid#15542DepositorAvailable SinceAug. 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-neoCV
Plasmid#73889Purposefission yeast drug selection markerDepositorInsertneoCV
ExpressionBacterial, Mammalian, and Y…PromoterCMVAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMalAnPL
Plasmid#67281PurposeExpresses MBP-Anacystis nidulans photolyase in E. coliDepositorInsertphotolyase Anacystis nidulans
Tagsmaltose binding proteinExpressionBacterialMutationnone knownAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOSV808
Plasmid#126601Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
Plasmid#172504PurposeEncodes the GwCas7x3 protein only for bacterial recombinant protein expression for purificationDepositorInsertGwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
ExpressionBacterialAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS11 AA 300-597
Plasmid#199329PurposeExpresses His-tagged Drosophila IntS11 amino acids 300-597DepositorInsertIntegrator subunit 11 (IntS11 Fly)
Tags6xHisExpressionBacterialMutationamino acids 300-597 onlyAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISMmu1_reRNA
Plasmid#205349PurposeBacterial expression of ISMmu1 TnpB and its reRNADepositorInsertISMmu1 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT254-litR
Plasmid#202308PurposelitR repressor plasmid for LPattB2 integrationDepositorInsertlitR
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJT249-psrA
Plasmid#202304PurposepsrA repressor plasmid for LPattB2 integrationDepositorInsertpsrA
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p3_mFAP2b
Plasmid#159470PurposeEncodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p3_mFAP2b
Tags6xHis-thrombin-TEVExpressionBacterialAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-mFAP10
Plasmid#159463PurposeEncodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertmFAP10
Tags6xHis-thrombin-TEVExpressionBacterialAvailable SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GGvT
Plasmid#87362PurposeExpresses GGvT, a tandem homodimer green fluorescent proteinDepositorInsertGGvT
TagsHis-TagExpressionBacterialAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC010 LshCas13a locus with nontargeting spacer
Plasmid#91901PurposeLshCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLshCas13a locus with non-targeting spacer
ExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
L3MBTL2
Plasmid#25244PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Bmx-SH2
Plasmid#46409DepositorInsertBmx (BMX Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 286-400PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-5/5
Plasmid#91464PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-5/5DepositorInsertSH3PXD2A-5/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-1/5
Plasmid#91351PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-1/5DepositorInsertSH3PXD2A-1/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-3/5
Plasmid#91352PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-3/5DepositorInsertSH3PXD2A-3/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS Rax (CC#5)
Plasmid#15138DepositorInsertRax (Rax Mouse)
ExpressionBacterialAvailable SinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
ExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST TopBP1 B (aa 978-1192)
Plasmid#20372DepositorAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
B5-2
Plasmid#111481PurposeArg-tRNA synthetaseDepositorInsertArg-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL1270
Plasmid#129281PurposeThe vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellumDepositorTypeEmpty backboneExpressionBacterialPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb68
Plasmid#159423PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68DepositorInsertSb68
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1376 (Bcl-2 P1 promoter with MHS1234)
Plasmid#15387DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 P1 promoter with IgH full enhancer MHS1234Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
Pv-FDPS
Plasmid#25554PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPv-FDPS
TagsHisExpressionBacterialAvailable SinceSept. 10, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2-cryR;dll4CE1-P1Egfp
Plasmid#90146Purposecontains dll4 enhancer 1 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTKIP-hph
Plasmid#41066DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-GST-Laforin
Plasmid#84673Purposebacterial expression, GST-fusion proteinDepositorArticleAvailable SinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
CUL3A-c012
Plasmid#53673Purposebacterial expression plasmidDepositorInsertCUL3A
ExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C NLRP1 CARD WT
Plasmid#164008PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARDDepositorAvailable SinceFeb. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT891
Plasmid#68932PurposeInt8 expressed constitutively from PAM4 and the rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertInt8
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only