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pGBW-m4046901
Plasmid#145725PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLC-239
Plasmid#73193PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-yhaV (yhaV )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-2
Plasmid#12994DepositorInsertTSP-2 (THBS2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-4u
Plasmid#13553DepositorInsertFLIPPi T163A
UseTagsECFP and EYFPExpressionBacterialMutationT163A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pRS306:VN
Plasmid#37555DepositorInsertVenus N-term (aa 1-272)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO BROX 1-411;
Plasmid#89602PurposeExpresses full length human Brox in bacteria; internal ID WISP11-297DepositorInsertBROX (BROX Human)
UseTagsHis Tag, V5 tagExpressionBacterialMutationPromoterT7Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
CXXC1
Plasmid#32045PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCXXC1 (CXXC1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationCXXC domain (amino acids residues 161-222)PromoterAvailable sinceJan. 3, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMB1-D1-TelR
Plasmid#204983PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SIAH2_Sina
Plasmid#109813PurposeProtein expression and purification of SIAH2_SinaDepositorInsertSIAH2_Sina (SIAH2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZa-tetO-csgAW-CmR
Plasmid#98937PurposeConductive Curli Protein csgAWDepositorInsertcsgAW
UseTagsWWRKWKEWDDWExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZa-tetO-csgAY-CmR
Plasmid#98938PurposeConductive Curli Protein csgAYDepositorInsertcsgAY
UseTagsYYRKYKEYDDYExpressionBacterialMutationPromotertetOAvailable sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRF-TarA
Plasmid#49373DepositorInsertTarA repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-AP
Plasmid#61830PurposeEncodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPullDepositorInsertSNAP-AP
UsePcr templateTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS Survivin
Plasmid#19233DepositorInsertSurvivin (BIRC5 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_31
Plasmid#109235PurposePUPsp1 GFP reporterDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPUPsp1Available sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
4RFQ
Plasmid#64656PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL18:PBC004-E03:C231318 (METTL18 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceMay 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationR399EPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tyk2-SH2
Plasmid#46527DepositorInsertTyk2 (TYK2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 452-567PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PGEX-6P1-hNF2-AR mutant
Plasmid#107149Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTagsGSTExpressionBacterialMutationA585W-R588KPromoterPtacAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCML 961
Plasmid#98848PurposepTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).DepositorInserts5' UTR + first 100 nucleotides of coding sequence of the aidB gene
csrA
UseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET/LEGH
Plasmid#108226PurposeBacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tagDepositorInsertLBT-EGFP-His6
UseTagsExpressionBacterialMutationPromoterT7, lacOAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD)
Plasmid#176191PurposegfcD protein expressionDepositorInsertgfcD
UseTags6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcD-10xHis)
Plasmid#176192PurposegfcD protein expressionDepositorInsertgfcD
UseTags10xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcB)
Plasmid#176196PurposegfcB protein expressionDepositorInsertgfcB
UseTags6xHis-TeVExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG26(gfcD)
Plasmid#176198PurposegfcD protein expressionDepositorInsertgfcD
UseTags10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcB-His-CD)
Plasmid#176194Purposeexpresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
UseTagsGfcB-6xHisExpressionBacterialMutationPromoterPtacAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD)
Plasmid#176199PurposeEncodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBH31(gfcD-10xHis)
Plasmid#176200PurposeEncodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasmDepositorInsertgfcD
UseTagspelC leader-10xHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSA10(gfcBCD)
Plasmid#176193Purposeexpresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operonDepositorInsertgfcBCD
UseTagsExpressionBacterialMutationPromoterPtacAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-RevA-ss
Plasmid#137064PurposeE. coli expression clone (T7lac promoter) for mature RevA (aa 25-160) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationlacks the RevA signal peptide and 5 residues (CKA…PromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSM42
Plasmid#113942PurposeConstitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1aDepositorInsertjRGECO1a
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - GR
Plasmid#89614PurposeExpresses recombinant 6HIS tagged T. cruzi Glutathione reductase protein in E. coli upon IPTG inductionDepositorInsertGlutaredoxin
UseTags6HISExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLlacO1-lacI-T500
Plasmid#45393DepositorInsertlac repressor
UseTagsExpressionBacterialMutationPromoterPL-lacO1Available sinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pR6Kcat-rdxA-rpsL
Plasmid#45471DepositorInsertcat-rdxA-rpsL
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIGH
Plasmid#112804Purposegateway cloning destination vector to express enhancer driven Gal4 in DrosophilaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K410N
Plasmid#87280PurposeEncodes Hsh155 K410N for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationK410NPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_EBFP2
Plasmid#118853Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertEBFP2
UseTagsHis-tagExpressionBacterialMutationEBFP1.2-I128V/V150I/ D155V/V224RPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW414hrpL-cIgfp
Plasmid#61439PurposepSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs34-CI-LVA-t-plam-30gfp-t
UseTagsLVAExpressionBacterialMutationPromoterHrpLAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASK-mSAN1-Streptag
Plasmid#117165PurposeExpresses Strep-tagged murine SAN1 nuclease in bacteriaDepositorInsertmSAN1 (Fam120b Mouse)
UseTagsStreptagExpressionBacterialMutationPromotertetR/tetAAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCAM35
Plasmid#86475PurposeExpresses p9-6HisDepositorInsertp9-6His
UseSynthetic BiologyTags6HisExpressionBacterialMutationPromoterpTetAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMGU
Plasmid#139933PurposeM. magneticum/E. coli shuttle vector (pMGT and pUC)DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23101Available sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only
