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Showing: 11401 - 11450 of 29646 results

  6. pET mClover LIC cloning vector (H6-mClover)

    Plasmid
    #86931
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mC…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86931-plasmid-map-sequence-id-174751

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  9. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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  16. O-IL8-15

    Plasmid
    #166544
    Purpose
    scFv of a human scaffold targeting Interleukin-8. Antigen coverage aa 28-99 of 99, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166544-plasmid-map-sequence-id-328277

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  21. pHL 636

    Plasmid
    #53015
    Purpose
    AmpR + PLtetO-1::RBS (st7) fimE::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Inserts
    fimE
    fimB
    Use
    Synthetic Biology
    Tags
    Asp terminator, RBS(st7), and T1 terminator
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    53015-plasmid-map-sequence-id-116679

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  22. pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant

    Plasmid
    #101231
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N692A, M694A, Q695A an…
    Promoter
    T7
    Available since
    Nov. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    101231-plasmid-map-sequence-id-197986

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  30. PP-HSP90 (3H80)

    Plasmid
    #25273
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LmjF33.0312:M1-K213
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-K213
    Promoter
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pGEX-MK4(6)

    Plasmid
    #30189
    Depositor
    Bruce Tempel
    Insert
    Kcna6 (Kcna6 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. pAL725

    Plasmid
    #119862
    Purpose
    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Leucine 244 to Cysteine, Aspartate 287 to Cysteine
    Promoter
    T5
    Available since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119862-plasmid-map-sequence-id-232032

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  37. pTvHR21-SGC

    Plasmid
    #39186
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Use
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

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  43. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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Showing: 11401 - 11450 of 29646 results