31,059 results
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Plasmid#213860PurposeFor Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertmCherry-sfGFP
ExpressionBacterial and PlantPromoterCaMV 35SAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIT5_CL
Plasmid#173767PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHH0103_PIK3R2-1/1
Plasmid#91415PurposeProtein expression and purification of human SH3 domain construct PIK3R2-1/1DepositorInsertPIK3R2-1/1 (PIK3R2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP-MMP
Plasmid#112633PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP-MMP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNMT
Plasmid#25248PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL
Plasmid#64177Purposeharbours marker gene LEU2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-soNadV
Plasmid#83363PurposeExpresses nadV from Shewanella oneidensis in Escherichia coliDepositorInsertnadV
ExpressionBacterialAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA1
Plasmid#39021PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET29b-ClbP
Plasmid#48244PurposeUntagged, full-length protein for in vivo peptidase assaysDepositorInsertClbP
TagsNoneExpressionBacterialPromoterT7Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #3
Plasmid#104206PurposeBacterial expression of WW domain #3 from NEDD4LDepositorInsertNEDD4L WW domain #3 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY8
Plasmid#24663DepositorInsertmCherry
ExpressionBacterialAvailable SinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB147
Plasmid#101662PurposeOpto-T7RNAP*(302)DepositorInsertOpto-T7RNAP*(302)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM
Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RPA2_RPA-C
Plasmid#109795PurposeProtein expression and purification of RPA2_RPA-CDepositorInsertRPA2_RPA-C (RPA2 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL1-PD-4xDQNAT
Plasmid#128391PurposeIn vitro expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sitesDepositorInsertProtein D
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22-6His-SUMO-Nsp10
Plasmid#154960PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 21, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIT5_KH_VanR
Plasmid#173779PurposeBacterial genomic integration vector, VanR repressorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonExpressionBacterialMutationTGTTGATA --> TGTTGGAAAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7(gfcC)
Plasmid#176197PurposegfcC protein expressionDepositorInsertgfcC
Tags6xHis-TeVExpressionBacterialPromoterT7Available SinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-SCP1-ccdB
Plasmid#131628PurposeModified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)DepositorTypeEmpty backboneUseLuciferaseExpressionBacterial and MammalianPromoterSCP1Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-90) (72P)
Plasmid#62080PurposeExpression of human p53 (1-90) (72P) in e. coliDepositorInsertp53 (TP53 Human)
TagsHisExpressionBacterialMutationcontains TAD/PP residues 1-90; 72PPromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-Lux-nat
Plasmid#120323PurposeBacterial luminescence promoter trapDepositorInsertLux operon
ExpressionBacterialPromoter(None)Available SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ECH1
Plasmid#38911PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSG578
Plasmid#214215Purposeintegrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCBe
Plasmid#110065PurposeTrc1O promoter express gene in Synechocystis 6803DepositorTypeEmpty backboneTags3' end His tag and 5' end FLAG tagExpressionBacterialPromoterTrc1OAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB089
Plasmid#98914PurposeExpress Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coliDepositorInsertsFtsZ
ZapA
TagsDronpa and pAmCherry1ExpressionBacterialPromoterT5-lacAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAPK11
Plasmid#39005PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-CTD
Plasmid#136649PurposeExpression of SH3BP1-CTD-GST fusion protein in E. coliDepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1-NTD
Plasmid#136648PurposeExpression of SH3BP1-NTD-GST fusion protein in E. coliDepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
S2-77 pComb3xss anti-cFos nanobody
Plasmid#145834PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S2-77 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-PelB-NH10T-AVITAG-GG
Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-B-frSkyran-S
Plasmid#182209PurposeBacterial expression of frSkylan-SDepositorInsertfrSkylan-S
Tags6xHisExpressionBacterialPromoterT7Available SinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS15k-RFP
Plasmid#126194PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD mPlum
Plasmid#91773PurposeBacterial expression of a fluorescent protein, monomeric Plum (mPlum)DepositorInsertmonomeric Plum
Tagsno tagExpressionBacterialPromoteraraBADAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AKT1_Pkinase
Plasmid#109775PurposeProtein expression and purification of AKT1_PkinaseDepositorInsertAKT1_Pkinase (AKT1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (OTU, aa 287-437)
Plasmid#61407PurposeExpresses human OTUD1 (OTU domain) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-286 and aa 438-481.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExp-Calm
Plasmid#129238PurposeProtein expression in E.coliDepositorTypeEmpty backboneTagsHis and StrepExpressionBacterialPromoterT7Available SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a(DAP10)
Plasmid#177904PurposeBacterial expression of his10-tagged human DAP10 Intracellular DomainDepositorInsertDAP10 (aa 70-92 only) (HCST Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA-GFP
Plasmid#133009PurposepFA carrying a sfGFP geneDepositorInsertsfGFP
Tags6xhisExpressionBacterialPromotertetRAvailable SinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PipaH-TetR
Plasmid#97100PurposeExpresses TetR in the presence of ipgC and mxiEDepositorInsertTetR
ExpressionBacterialPromoterPipaHAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
T7pr-His6-MBP-TEV-dCas9-BirA*
Plasmid#159994PurposeBacterial expression of dCas9-BirA*DepositorInsertdCas9-BirA*
Tags6X-His, MBP, 3X-FLAG, NLSExpressionBacterialMutationD10A, H840APromoterT7Available SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL8B-V5-His6
Plasmid#67425PurposeExpression of hArl8b with C-term V5 and His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-RFP
Plasmid#35282DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterlacUV5Available SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDF-KpAvs4
Plasmid#188807PurposeCodon optimized KpAvs4 with C-terminal His tagDepositorInsertKpAvs4
TagsHisExpressionBacterialPromoterT7Available SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only