32,684 results
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CL7_2xNLS_iGeoCas9(G)_2xNLS_His
Plasmid#222989PurposeBacterial expression of iGeoCas9(G) constructDepositorInsertCL7_2xNLS_iGeoCas9(G)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM
Plasmid#221379PurposeExpresses FANCM in bacterial cellsDepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB328
Plasmid#113672Purposeexpresses Ulp1_R4 in Escherichia coliDepositorInsertUlp1_R4
Tags6-HisExpressionBacterialMutationcontains solubility enhancing mutationsPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT-4
Plasmid#22831DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
ACS2 Truncated
Plasmid#13748DepositorInsertTruncated Acetyl CoA Sythetase 2 (Acss1 Mouse)
TagsHisExpressionBacterialMutationContains amino acids 39–682Available SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCJH059_proD_Cas12c_DualRNA-GFP_CAM
Plasmid#183076PurposePlasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.DepositorInsertCas12c tracrRNA and pre-crRNA
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH009_proD_Cas12c-sgRNA-GFP_CAM
Plasmid#183070PurposePlasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria.DepositorInsertCas12c single guide RNA targeting GFP
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P13-ss
Plasmid#137065PurposeE. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P13: lacks the P13 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1R1AtR2B2
Plasmid#160880PurposeExpression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1 and A. thaliana rubisco chaperone proteins raf2, and bsd2 in E. coli.DepositorInsertrbcS-S1, rbcX, raf1, raf2, bsd2
ExpressionBacterialPromoterrbcS-S1: T7, chaperones: T7Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
DRP2A
Plasmid#42354PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRF-LitR
Plasmid#49363DepositorInsertLitR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATAD2B
Plasmid#39046PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB082b
Plasmid#79234PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d3
ExpressionBacterialPromoterPpro1Available SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-IntNtr-eSrt2A
Plasmid#126015PurposeBacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-IntNtr-eSrt2A
Tags6xHis-SUMOExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-E138A
Plasmid#69921PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
TagsFLAGExpressionBacterial and MammalianMutationE138APromoterCMVAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
GOT1
Plasmid#39022PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
RAB9B
Plasmid#25625PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRF-ScbR
Plasmid#49370DepositorInsertScbR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOPO ZeoR
Plasmid#68446PurposeTOPO construct expressing ZeoR for generation of GMAP-compatible geneDepositorInsertZeocin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDC20
Plasmid#131366Purposeexpression of MEG-3 (aa1-862) in C. elegansDepositorAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean ME
Plasmid#103971PurposePlasmid encoding mCerulean MEDepositorInsertmCerulean ME
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRGM1
Plasmid#48789PurposeHA-I-SceI expression plasmid, episomalDepositorInsertI-SceI
TagsHA tagExpressionBacterialPromoterMOPAvailable SinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
GCN2-KD1-pGEX4T-1
Plasmid#21876DepositorAvailable SinceSept. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_18
Plasmid#59953PurposeQuad split (at 61, 179, 601-SZ)DepositorInsertFour-fragment T7* RNA polymerase
ExpressionBacterialMutationN/APromoterPTacAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4P1Egfp
Plasmid#90160Purposecontains dll4 specific gene promoter driving expression of eGFPDepositorAvailable SinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-TPR
Plasmid#190822PurposeFor expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationC467X. Also codon optimized for expression in E.c…PromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
KaiC
Plasmid#41884DepositorInsertKai C
TagshistadineExpressionBacterialPromotert7Available SinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOSV812
Plasmid#126605Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK033
Plasmid#65140PurposeEncodes Venus as a Type 3 part to be used in the Dueber YTK systemDepositorInsertVenus
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA2-RFP
Plasmid#62248Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA2Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJUMP48-2A(sfGFP)
Plasmid#126993PurposeLevel 2 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLux-LmrA
Plasmid#49375DepositorInsertLmrA repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDF0044 CjcCas7-11 full locus with synthetic array
Plasmid#172502PurposeEncodes the CjcCas7-11 full locus for bacterial expressionDepositorInsertCjcCas7-11 full locus with synthetic array
ExpressionBacterialAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TOPO HygroR
Plasmid#68445PurposeTOPO construct expressing HygroR for generation of GMAP-compatible geneDepositorInsertHygromycin Resistance
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMTL-4
Plasmid#22814DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET_scFv_QLuc
Plasmid#183261PurposeExpress scFv_QLuc in bacteria cellsDepositorInsertscFv_QLuc
TagsHistagExpressionBacterialPromoterT7Available SinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM002
Plasmid#170006PurposeEncodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyExpressionBacterialPromoterPLTetO-1 and PpmrDAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-IBBN (G2)
Plasmid#85500PurposePlasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertIBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32G L65E F108W Q109M D158S R162KPromoterlpp (constitutive)Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Nkx6.2
Plasmid#15542DepositorAvailable SinceAug. 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
ExpressionBacterialPromotercLac143Available SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_E83K
Plasmid#109412PurposeRepA MutantDepositorInsertrepA
TagsRFPExpressionBacterialMutationE83K repA mutation to increase copy numberAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-tTALE-mStrawberry-15b
Plasmid#206868PurposeExpress tTALE-mStrawberryDepositorInsert7-mer tTALE-mStrawberry
ExpressionBacterialPromoterT7 promoterAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
PET.SUMO eIF4G1 1-200
Plasmid#158791PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.DepositorInserteIF4G1 (codon optimized)
Tags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDB026e
Plasmid#109261PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPpsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
ExpressionBacterialMutationamber codon at position 29 and 184 of gIIIPromoterPpsp and PproKAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRBBm69
Plasmid#48116PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - sacB
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only
