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Plasmid#171324PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertFirefly luciferase reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
22 pCOLI_A_N-MBP_C_STREP
Plasmid#160164PurposeN-term MBP 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepII, 6xHis, and MBPExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a- hPTP1e-PDZ2
Plasmid#20377DepositorInserthPTP1e PDZ2 (PTPN13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pBbS1a-RFP
Plasmid#35337DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterTrcAvailable sinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET B-CKAR
Plasmid#14868DepositorInsertC kinase activity reporter
UseTags6xHis/Xpress, CFP, and YFPExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mqgDUX4
Plasmid#21176DepositorInsertDUX4 (DUX4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsYTHDC2_36-1430_AD
Plasmid#148487PurposeBacterial Expression of HsYTHDC2_36-1430DepositorInsertHsYTHDC2_36-1430 (YTHDC2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TP53BP2-1/1
Plasmid#91503PurposeProtein expression and purification of human SH3 domain construct TP53BP2-1/1DepositorInsertTP53BP2-1/1 (TP53BP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_STAC3-2/2
Plasmid#91466PurposeProtein expression and purification of human SH3 domain construct STAC3-2/2DepositorInsertSTAC3-2/2 (STAC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3GL1-1/1
Plasmid#91473PurposeProtein expression and purification of human SH3 domain construct SH3GL1-1/1DepositorInsertSH3GL1-1/1 (SH3GL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VAV2-1/2*
Plasmid#91483PurposeProtein expression and purification of human SH3 domain construct VAV2-1/2*DepositorInsertVAV2-1/2* (VAV2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SASH3-1/1
Plasmid#91434PurposeProtein expression and purification of human SH3 domain construct SASH3-1/1DepositorInsertSASH3-1/1 (SASH3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YES1-1/1
Plasmid#91439PurposeProtein expression and purification of human SH3 domain construct YES1-1/1DepositorInsertYES1-1/1 (YES1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_STAC3-1/2
Plasmid#91271PurposeProtein expression and purification of human SH3 domain construct STAC3-1/2DepositorInsertSTAC3-1/2 (STAC3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
Plasmid#127790PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR L1-13XLexAop2-R5
Plasmid#41433DepositorInsert13XLexAop2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS8k-RFP_R43W
Plasmid#109416PurposeRepA MutantDepositorInsertrepA
UseTagsRFPExpressionBacterialMutationR43W repA mutation to increase copy numberPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-HA-CPDSalI
Plasmid#38253DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYUB28b-MCS-optimized
Plasmid#89179Purposemycobacteria shuttle vector - pYUB28b with a single change to the MCSDepositorTypeEmpty backboneUseMycobacteria shuttle vectorTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7034
Plasmid#133869PurposeAtzA expression plasmidDepositorInsertAtzA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7035
Plasmid#133870PurposeAtzB expression plasmidDepositorInsertAtzB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP384
Plasmid#158799PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purificationDepositorInsertdCasPhi-3 (D413A)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJC175e-DddI
Plasmid#179693PurposeMutagenesis plasmid with constitutive bacterial expression of DddI immunity proteinDepositorInsert[SD8] gIII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_036
Plasmid#109153PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2DepositorInsertsmRFP
mKO2
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBD21_034
Plasmid#109151PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFPDepositorInsertsmRFP
TagBFP
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
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MSP2266
Plasmid#70705PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1_EHD1_438-534
Plasmid#36459DepositorInsertEHD1 EH domain (EHD1 Human)
UseTagsGSTExpressionBacterialMutationdeleted amino acids 1-437 from full-length constr…PromoterAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3186 HPV-16 E1
Plasmid#10868DepositorInsertHPV-16 E1 (E1 Human papillomavirus)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBMTBX-4
Plasmid#26075DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC
Plasmid#116966PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
MP-Q6
Plasmid#69676PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA ugi APOBEC1
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129L
Plasmid#36062DepositorInsertalpha-synuclein S129L (SNCA Human)
UseTagsExpressionBacterialMutationS129LPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-MAPRE1
Plasmid#31589DepositorInsertmicrotubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSR26-2
Plasmid#104335PurposeExpresses ompR from a TetR reuglated promoter under inducible ATC control.DepositorInsertOmpR (ompR Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterPLTetO1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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DDX19A
Plasmid#42419PurposeBacterial expression for structure determination; may not be full ORFDepositorUseTagsHis6-TEVExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pET30-2-GPD1L
Plasmid#83912Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
UseTags6x His tagsExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
(GGS)9_(+36GFP)-Cre.ape
Plasmid#71747Purpose+36-GFP-Cre fusion expression in E.ColiDepositorInsert(GGS)9_+36GFP-Cre
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -