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32,161 results

Showing: 11001 - 11040 of 32161 results

  2. pColdII-SIRPT1-Loxodonta Africana

    Plasmid
    #75351
    Purpose
    Nt His-tag Loxodonta Africana 89-1368
    Depositor
    Kalle Gehring
    Insert
    Sacsin Loxodonta Africana 89-1368
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75351-plasmid-map-sequence-id-142900

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  11. pETM33_Nsp8

    Plasmid
    #156477
    Purpose
    Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156477-plasmid-map-sequence-id-307732

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  14. pETM41-EcPPK

    Plasmid
    #38334
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Tags
    6xHIS, MBP, and TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 10, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  22. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  26. CBX2

    Plasmid
    #25158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX2_04 (CBX2 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25158-plasmid-map-sequence-id-472908

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  27. HERC2 (3KCI)

    Plasmid
    #25344
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    herc2.3951.4321 (HERC2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acid residues 3951-4321
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pOGG004

    Plasmid
    #113979
    Purpose
    Vector construction module. Level 1 golden gate cloning site module with LacZ reporter
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Level 1 golden gate cloning site
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113979-plasmid-map-sequence-id-223412

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  33. pXWJ109HgAmp30E

    Plasmid
    #123149
    Purpose
    Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Depositor
    Baojun Wang
    Article
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, PmerT, PhrpLE
    Available Since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123149-plasmid-map-sequence-id-244017

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  36. pB2H2*

    Plasmid
    #163834
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and TCPTP(R222M)
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    TCPTP R222M
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available Since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163834-plasmid-map-sequence-id-337601

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  38. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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Showing: 11001 - 11040 of 32161 results