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32,656 results

Showing: 1501 - 1550 of 32656 results

  4. pKD4_mCherry

    Plasmid
    #187386
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187386-plasmid-map-sequence-id-370396

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  9. pTOX8

    Plasmid
    #127453
    Purpose
    Encodes MqsR toxin and tsPurple chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    mqsR toxin
    tsPurple chromoprotein
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    apFAB46 and pRha
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127453-plasmid-map-sequence-id-251148

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  10. pExp-Trx

    Plasmid
    #112577
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TrxA and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112577-plasmid-map-sequence-id-217212

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  13. pET22b_PstS_2

    Plasmid
    #78199
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,A197C)
    Expression
    Bacterial
    Mutation
    A17C; A197C (please see depositor comment below)
    Promoter
    T7
    Available Since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78199-plasmid-map-sequence-id-146049

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  17. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    222080-plasmid-map-sequence-id-438352

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  19. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid
    #108693
    Purpose
    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9
    Inserts
    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Shine Dalgarno sequence upstream of start codon, …
    Promoter
    tac
    Available Since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    108693-plasmid-map-sequence-id-208987

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  20. SPLICSFAST-LDh-LY-P2A

    Plasmid
    #214895
    Purpose
    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-LY-P2A
    Tags
    none
    Expression
    Bacterial and Mammalian
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214895-plasmid-map-sequence-id-423481

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  28. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

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  34. pAD-LyseR

    Plasmid
    #99244
    Purpose
    For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    phage lambda gene R (R phage lambda)
    Expression
    Bacterial
    Promoter
    beta-lactamase promoter
    Available Since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99244-plasmid-map-sequence-id-191038

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  36. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  37. mEGFP-pBAD

    Plasmid
    #54622
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis and mEGFP
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54622-plasmid-map-sequence-id-257491

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  38. 5ULO (YWHAZ)

    Plasmid
    #101258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YWHAZ (YWHAZ Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101258-plasmid-map-sequence-id-197642

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  39. hFerritinH

    Plasmid
    #122652
    Purpose
    Expression of hFerritinH in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Expression
    Bacterial
    Available Since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122652-plasmid-map-sequence-id-237549

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  40. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  43. pTXB1-pA-M.EcoGII

    Plasmid
    #192873
    Purpose
    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578
    Depositor
    Chong Tang
    Article
    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:
    Insert
    pA-M.EcoGII
    Tags
    3X Flag Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    192873-plasmid-map-sequence-id-380474

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  44. pKD3_mCherry

    Plasmid
    #187385
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187385-plasmid-map-sequence-id-371123

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  46. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

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Showing: 1501 - 1550 of 32656 results