31,059 results
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Plasmid#55665Purposeexpression of mouse RAP1BDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only
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pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB211
Plasmid#228494Purpose10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)DepositorInsertpol
Tags10xHis-SUMO(Smt3)ExpressionBacterialMutationA408SPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7-CalfluxVTN
Plasmid#83926PurposeBioluminescent Reporter of Intracellular CalciumDepositorInsertCalfluxVTN
ExpressionBacterialMutationNonePromoterT7Available SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
RAP1A
Plasmid#55664Purposeexpression of mouse RAP1ADepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRE107
Plasmid#43829DepositorTypeEmpty backboneUseBacterial allelic exchange vector with sacb1ExpressionBacterialAvailable SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mSA
Plasmid#39860DepositorInsertmonomeric streptavidin
Tags6xHis and FLAGExpressionBacterialPromoterT7Available SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-Fluc
Plasmid#183474PurposeThis plasmid can be used to prepare Fluc mRNA by In Vitro TranscriptionDepositorInsertFirefly luciferase
UseLuciferaseExpressionBacterialPromoterT7Available SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIRCNpu
Plasmid#74226PurposeVector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.DepositorTypeEmpty backboneUseSynthetic BiologyTagsNpu DnaE C-intein and Npu DnaE N-inteinExpressionBacterialPromoterT7-lacAvailable SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneTags6xHis and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-45b-mCherry-NPM1
Plasmid#194546PurposeBacterial Expression of 6xHis mCherry-NPM1 Fusion ProteinDepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (2GFP-T)
Plasmid#29716DepositorTypeEmpty backboneTags6xHis, SiriusGFP, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-HotPETase
Plasmid#218701PurposeEncodes codon optimized HotPETase enzyme for bacterial expressionDepositorInsertHotPETase
Tags10xHisExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGR-L3S2P21
Plasmid#46004DepositorInsertL3S2P21 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYC01
Plasmid#224586PurposeIPTG-inducible phage φX174 lysis gene EDepositorInsertphage φX174 lysis gene E (phiX174p08 )
UseSynthetic BiologyExpressionBacterialPromoterPA1lacO-1Available SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-Z1-PETase
Plasmid#218703PurposeEncodes codon optimized Z1-PETase enzyme for bacterial expressionDepositorInsertZ1-PETase
Tags10xHisExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
TagsHis-TagExpressionBacterialPromoterTrcAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMtb ATR
Plasmid#138297PurposeExpression of Mtb ATRDepositorInsertCorrinoid adenosyl transferase
TagsHis tagExpressionBacterialPromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKO403_lacZ_Sp
Plasmid#174726PurposeBifidobacterium-E.coli shuttle vector for seamless gene cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-DogCatcher
Plasmid#171929PurposeExpress Cys-DogCatcher protein in bacterial cytoplasm. The cysteine enables convenient coupling to maleimide-dye or thiol-reactive resin.DepositorInsertCys-DogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTP1172
Plasmid#104161PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
Tags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAV24
Plasmid#67716PurposeParental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
ExpressionBacterialPromoterT7 RNA polymeraseAvailable SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
NSUN2
Plasmid#188059PurposeBacterial expression plasmid for human NSUN2; for activity testingDepositorAvailable SinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521-myc
Plasmid#196239PurposeN-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28.His.3C.86b.Tau2N4R
Plasmid#226399PurposeExpresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteriaDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK-PTPN1
Plasmid#197988PurposeExpression of MerTK Kinase Domain (wild-type) in E. coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM31
Plasmid#135491PurposeAgrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3DepositorInserthph SpunH2A/Bpr hph-mCerulean3
TagsmCerulean3ExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iR
Plasmid#83314PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iR
Tags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iR betwe…PromoterT5Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGRG36
Plasmid#16666DepositorInsertmTn7::MCS
ExpressionBacterialAvailable SinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTD-NTwinStrep_Km
Plasmid#45938Purposeuse to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Twin-StrepExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-pdh_RiboJ_mCherry_Bba_B0015
Plasmid#107581PurposeB. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialPromoterpdh promoter Bacillus megaterium DSM319Available SinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC scAB-GFP
Plasmid#104998PurposeLentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labelingDepositorInsertsingle chain Antibody against GCN4
UseLentiviralTagsGB1, HA tag, and sfGFPExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVV052
Plasmid#215303PurposeExpresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPRhaBAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK503
Plasmid#110616PurposeBTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 10, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBA707
Plasmid#186238PurposeKanR-BBR1 entry vector for HR donorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLacO-ISce1
Plasmid#58505Purposeprovides 256 Lac operator repeats adjacent to the ISce1 restriction siteDepositorInsertLac repeats adjacent to 6 copies of the ISce1 restriction site
ExpressionBacterialPromoterno promoterAvailable SinceOct. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJ1996_v2
Plasmid#140664PurposedCas9 & Csy4DepositorInsertdCas9 & Csy4
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ube1/PET21d
Plasmid#34965DepositorAvailable SinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
His6-EGFP-PH-PLCD1
Plasmid#183675PurposePtdIns(4,5)P2 biosensor for purification from bacteriaDepositorInsertPLCD1 (PLCD1 Human)
TagsHis6-EGFPExpressionBacterialMutationAmino acids 11-140PromoterT7Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only