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Bacterial Expression

29,692 results

Showing: 1501 - 1550 of 29692 results

  1. pSAM_Cam

    Plasmid
    #91570
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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  10. pDXinit

    Plasmid
    #110101
    Purpose
    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages
    Depositor
    Markus Seeger
    Article
    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110101-plasmid-map-sequence-id-211288

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  15. pCDB179-DuraPETase

    Plasmid
    #218699
    Purpose
    Encodes codon optimized DuraPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    DuraPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218699-plasmid-map-sequence-id-434102

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  17. pMSP3535

    Plasmid
    #46886
    Purpose
    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression
    Depositor
    Gary Dunny
    Article
    Bryan et al Plasmid. 2000 Sep;44(2):183-90.
    Type
    Empty backbone
    Use
    Nisin-inducible expression; gram-positive bacteri…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PnisA
    Available since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    46886-plasmid-map-sequence-id-457466

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  18. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-67070

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  20. pJL31

    Plasmid
    #227428
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Insert
    mWasabi
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227428-plasmid-map-sequence-id-455967

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  21. pJL32

    Plasmid
    #227429
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Insert
    tdTomato
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227429-plasmid-map-sequence-id-455968

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  23. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A
    Promoter
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  24. pYHY3

    Plasmid
    #216109
    Purpose
    aTC-inducible expression of secretory BT3630 SP-NanoLuc via P1TDP-A21 promoter-RBS pair in Bacteroides species.
    Depositor
    Shannon Sirk
    Article
    Yeh et al Nat Commun. 2024 Nov 11;15(1):9741. doi:
    Insert
    NanoLuc
    Use
    Synthetic Biology; E. coli-bacteroides shuttle ve…
    Tags
    3xFLAG tag and BT3630 signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    P1TDP-A21
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216109-plasmid-map-sequence-id-434372

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  27. pBAD-bARGSer-AxeTxe

    Plasmid
    #192473
    Purpose
    Second-generation bacterial acoustic reporter gene derived from Serratia
    Depositor
    Mikhail Shapiro
    Article
    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Inserts
    bARGSer
    AxeTxe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the gene Ser39006_001280 was deleted from the clu…
    Promoter
    Native promoter from Enterococcus faecium and pBAD
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    192473-plasmid-map-sequence-id-376957

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  31. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

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  38. (+)-Limonene Synthase

    Plasmid
    #112230
    Purpose
    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
    Depositor
    Daniel Oprian
    Article
    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17
    Insert
    (+)-Limonene Synthase
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-52
    Promoter
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112230-plasmid-map-sequence-id-214310

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  44. pET_BGL3

    Plasmid
    #101912
    Purpose
    Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.
    Depositor
    Steve Shih
    Article
    Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944
    Insert
    β-glucosidase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7+LacO
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    101912-plasmid-map-sequence-id-198935

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  46. pDonor_RB-TnV2

    Plasmid
    #213907
    Purpose
    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    James Collins
    Article
    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.
    Insert
    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213907-plasmid-map-sequence-id-422622

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  47. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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Showing: 1501 - 1550 of 29692 results