32,838 results
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Plasmid#190156PurposeRegulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneTagsHis6 tagExpressionBacterialAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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pET-Duet1-His-TEV-mCherry-OPTN WT
Plasmid#190191PurposePlasmid for the expression of His-TEV-mCherry-OPTN WT. Internal reference: SMC797DepositorAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
ExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
His_Naa80d in pTYB12
Plasmid#188455PurposeExpress in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.DepositorAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6_rpoA-C
Plasmid#190966PurposeTest the C-to-U editing activity of KP6 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
TagsHisExpressionBacterialPromoterT7Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_zeo
Plasmid#184910PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_hph
Plasmid#184919PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKD317.8
Plasmid#181951PurposeIPTG-inducible expression of NarXDepositorExpressionBacterialPromoterNarX-Ptac; lacI-PlacIqAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-LuxA
Plasmid#188305PurposeExpression of LuxA via a T7 promoter in TXTLDepositorInsertluxA
UseLuciferase and Synthetic BiologyExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTXTL-T7max-HiBiTGFPHis
Plasmid#188317PurposeExpression of HiBiT-GFP via T7 promoter in TXTLDepositorInsertHiBiT-GFP fusion
UseLuciferase and Synthetic BiologyTags8x His-tagExpressionBacterialPromoterT7MaxAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero7
Plasmid#187926PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero7DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero9
Plasmid#187927PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero9DepositorInsertHero9
TagsFlag-6xHisExpressionBacterialAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero13
Plasmid#187929PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero13DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero20
Plasmid#187930PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero20DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_6xHis-TEV-mCh-NDP52
Plasmid#187829PurposePlasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148DepositorInsertNDP52 (CALCOCO2 Human)
Tags6xHisTag, TEV, and mCherryExpressionBacterial, Insect, and Mamm…Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnR9-10
Plasmid#182567PurposeHuman fibronectin fragment, type-III repeats 9-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 9-10
TagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379AAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
Tags6xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
Tags8xHis and EGFPExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPJ23116-MS2-rep
Plasmid#184842PurposeExpression of the rep protein of phage MS2DepositorInsertReplicase gene MS2 phage
UseSynthetic BiologyExpressionBacterialPromoterJ23116Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-SKW-HHL
Plasmid#190632PurposeBacterial expression of genetically encoded potassium indicator SKW-HHLDepositorInsertSKW-HHL
ExpressionBacterialAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a-LaM4-1xTS
Plasmid#162778PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-163
Plasmid#118542PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA789
Plasmid#186244Purposesoc-F HR Donor Vector (T4 phage editing)DepositorInsertBBR1 backbone + T4 phage homology
ExpressionBacterialAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.134
Plasmid#187219PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectivelyDepositorInsertA: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseSynthetic BiologyExpressionBacterialPromoterA: pTet*; B: pBetIAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only