29,693 results

1. pet-MBP

   Plasmid

   #209184

   Purpose

   Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Type

   Empty backbone

   Use

   Tags

   HisX6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX iLOV

   Plasmid

   #26587

   Depositor

   John Christie

   Article

   Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

   Insert

   iLOV
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1-N-FLAG

   Plasmid

   #119759

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   FLAG and GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPINM

   Plasmid

   #26044

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   His-MBP-3C-lacZ
   

   Use

   Tags

   MAHHHHHHSSG_MBP_LEVLFQGP

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAJM.474

   Plasmid

   #108526

   Purpose

   LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LuxR + PLuxB-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VanR: T49I A111V P179S

   Promoter

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

   Plasmid

   #198753

   Purpose

   Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSb_init containing the MBP sybody Sb_MBP#1

   Plasmid

   #132699

   Purpose

   expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   sybody_MBP#1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-His6-GFP-C-LACE

   Plasmid

   #133913

   Purpose

   Bacterial expression of His6-GFP-C-LACE

   Depositor

   Jeffrey Bode

   Article

   Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15-UbE2D1

   Plasmid

   #15782

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
   

   Use

   Tags

   6x His, T7 tag, and Xpress tag (enterokinase clea…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mApple-pBAD

    Plasmid

    #54536

    Purpose

    Localization: Protein Expression Vector , Excitation: 568, Emission: 592

    Depositor

    Roger Tsien, Nathan Shaner, Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUCP20T-dsRedExpress

    Plasmid

    #78470

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HRAS

    Plasmid

    #55653

    Purpose

    expression of mouse HRAS

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    HRAS (Hras Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30a_HIV-RT

    Plasmid

    #153311

    Purpose

    Bacterial expression of HIV reverse transcriptase

    Depositor

    Bryan Dickinson, Drew Endy

    Insert

    HIV Reverse Transcriptase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pKM444

    Plasmid

    #108319

    Purpose

    For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    Che9C RecT
    Bxb1 Integrase
    Tet Repressor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAP-delta10-cHIS

    Plasmid

    #21716

    Depositor

    Andrew Bohm

    Article

    Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

    Insert

    polyA polymerase (PAP1 Budding Yeast)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    30 amino acids off the c terminus

    Promoter

    Available since

    Aug. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pDEST14-SpyCatcher002

    Plasmid

    #102827

    Purpose

    Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hFerritinL

    Plasmid

    #122653

    Purpose

    Expression of hFerritinL in bacterial cells

    Depositor

    Trevor Douglas

    Insert

    light chain ferritin, human (FTL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE-WT-RGVG-M6

    Plasmid

    #70172

    Purpose

    Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

    Depositor

    Andrew Ellington

    Article

    Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

    Insert

    RGVG-M6 T7 RNA polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T5/Lac

    Available since

    Dec. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. hPU.1ΔN165-pET28b

    Plasmid

    #199796

    Purpose

    Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

    Depositor

    Gregory Poon

    Article

    Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

    Insert

    spi1 (SPI1 Human)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBFC0619

    Plasmid

    #186690

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. OmpA-mNG-sfTq2ox

    Plasmid

    #124220

    Purpose

    Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    OmpA-mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMLBAD

    Plasmid

    #32055

    Purpose

    Vector optimized for inducible gene expression in Burkholderia

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCdrA-gfp(ASV)C

    Plasmid

    #111615

    Purpose

    Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

    Depositor

    Tim Tolker-Nielsen

    Article

    Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

    Insert

    PcdrA-RBSII-gfp(ASV)-T0-T1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NFAT/AP-1 3x luciferase

    Plasmid

    #11783

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    NFAT/AP-1 3x (Il2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pUC19/human IL-8

    Plasmid

    #17610

    Depositor

    Joost Oppenheim

    Article

    Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

    Insert

    IL8 (CXCL8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNH-TrxT

    Plasmid

    #26106

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Tags

    6xHis, TEV cleavage site, and TrxA

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

    Plasmid

    #191457

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    His-mScarletI-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pspCas9-Klenow-ST2-com-vector

    Plasmid

    #176235

    Purpose

    Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pCM271rfp

    Plasmid

    #59774

    Purpose

    pCMrfp with TrfA mutation (271R to C)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET22b-T5-sfGFP*- MmH-MH4R

    Plasmid

    #196409

    Purpose

    Express sfGFP with TAG and enzymes for biosynthesizing DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    sfGFP with TAG at 151 position
    Tyrosine Hydroxylase (Th Mouse)
    dihydropterine reductase from human (QDPR Human)
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJMK001

    Plasmid

    #33780

    Depositor

    Adam Cohen

    Article

    Kralj et al Science. 2011 Jul 15;333(6040):345-8.

    Insert

    Proteorhodopsin Optical Proton Sensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D97N

    Promoter

    AraBAD

    Available since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV/SB11

    Plasmid

    #26552

    Depositor

    Perry Hackett

    Article

    Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

    Insert

    Sleeping Beauty
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Methionine 243 to Glutamine Threonine 136 to Arg…

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAJM.771

    Plasmid

    #108534

    Purpose

    NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    NahRAM + PSalTTC-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NahR: I25V P72H V118A R248C

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET-mKelly2

    Plasmid

    #119783

    Purpose

    Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

    Depositor

    Stephen Mayo

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

    Insert

    mKelly2
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. p2CT-His-MBP-Lwa_Cas13a_WT

    Plasmid

    #91865

    Purpose

    Bacterial expression for Cas13a

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

    Insert

    LwaCas13a
    

    Use

    CRISPR

    Tags

    His6-MBP-TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pPCK

    Plasmid

    #133128

    Purpose

    a recombinant plasmid, expressing pckA PEP-carboxykinase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    PEP-carboxykinase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEP-carboxykinase promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBBR1k-J23107-GFPuv

    Plasmid

    #106389

    Purpose

    pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

    Plasmid

    #191621

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pVC-Ds-E1b:eGFP-Ds

    Plasmid

    #102417

    Purpose

    Fluorescent vector for screening enhancer elements in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    E1b:eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish E1b minimal promoter

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRha-ABE8e-SpCas9-NG

    Plasmid

    #201190

    Purpose

    Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

    Depositor

    Jonathan Yen

    Article

    Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

    Insert

    ABE8e-SpCas9-NG
    

    Use

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    pRhaBAD

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pNCS-mGold2t

    Plasmid

    #231766

    Purpose

    Expression of mGold2t in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pNCS-mGold2s

    Plasmid

    #231765

    Purpose

    Expression of mGold2s in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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