30,008 results

1. pET-BoNT/X

   Plasmid

   #168017

   Purpose

   Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   BoNT/X-LC(1-439)-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC27

   Plasmid

   #117478

   Purpose

   mobilizable, SRB-stable, kanamycin-resistant cloning vector

   Depositor

   Judy Wall

   Article

   Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SBFP2-anti-AlfaTag nanobody

   Plasmid

   #171826

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   SBFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. sfTq2ox-PBP5

   Plasmid

   #117960

   Purpose

   Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   sfTq2ox-PBP5 (dacA )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SUMO_MT3

   Plasmid

   #200303

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

   Insert

   metallothionein 3 (MT3 Human)
   

   Use

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1delZn2delBRCT

   Plasmid

   #173939

   Purpose

   Bacterial expression of PARP1 lacking Zn2 and BRCT domains

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2delBRCT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 and 374-520; V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK556

   Plasmid

   #74277

   Purpose

   Produces formylglycinamide ribonucleotide amidotransferase, N-terminal His6 tag (H6EcPurL)

   Depositor

   T. J. Kappock

   Insert

   formylglycinamidine ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes alanine-1246 to serine, numerous silent m…

   Promoter

   T7

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAKgfplux3

   Plasmid

   #199304

   Purpose

   Fluorescence and bioluminescence expression in Gram-negative bacteria

   Depositor

   Attila Karsi

   Article

   Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

   Insert

   Chloramphenicol acetyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6

   Plasmid

   #180428

   Purpose

   Genome engineering of Agrobacterium

   Depositor

   Jun Fu

   Article

   Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

   Insert

   RecETh1h2h3h4_agrob6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter / tac promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCR-Zeo

    Plasmid

    #158709

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMW363

    Plasmid

    #238208

    Purpose

    Expresses TasR with an N-terminal Twin-StrepII tag, followed by 3xRNA1 TIGR array. AmpR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    TwinStrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMW364

    Plasmid

    #238205

    Purpose

    Expresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR array

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sfGFP-pBAD

    Plasmid

    #54519

    Purpose

    Localization: Protein Expression Vector , Excitation: 485, Emission: 507

    Depositor

    Geoffrey Waldo, Michael Davidson

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and SfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AARS_C1_pNIC-Bio3

    Plasmid

    #153046

    Purpose

    Expression construct for producing Avi tagged Alanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    AARS (AARS Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P1-hcGAS(157-522)

    Plasmid

    #108676

    Purpose

    For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

    Insert

    cGAS amino acid 157-522 (CGAS Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains amino acid 157-522

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCPP5052

    Plasmid

    #128729

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopAM1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMTS-hFluc

    Plasmid

    #177716

    Purpose

    Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Use

    Tags

    Mitochondrial Targeting Sequence MTS SS

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_VASP_WH1

    Plasmid

    #109790

    Purpose

    Protein expression and purification of VASP_WH1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    VASP_WH1 (VASP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MP1

    Plasmid

    #69627

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 umuD' umuC recA730
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZP06C

    Plasmid

    #232322

    Purpose

    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

    Depositor

    Chenggang Wu

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsJ

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-26b-Nb127D01-HA-His

    Plasmid

    #171566

    Purpose

    Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-HA-His (CXCR2 Human)
    

    Use

    Tags

    HA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMBP-hLIF

    Plasmid

    #179711

    Purpose

    Expresses H6-MBP-LIF

    Depositor

    Mark Arbing

    Insert

    Leukemia inhibitory factor (LIF Human)
    

    Use

    Tags

    His6-MBP-TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MICU-APEX2

    Plasmid

    #79057

    Purpose

    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    C-terminal APEX2 fused MICU1 (MICU1 Soybean, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV immearly promotor

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGEX-5X-TRIM28

    Plasmid

    #45570

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge