32,115 results
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Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
TagsMBPExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1159
Plasmid#119250Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUA66 theta GFP
Plasmid#105607PurposeExpresses GFP with no promoterDepositorInsertGFP
ExpressionBacterialPromoterNo PromoterAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCYP58
Plasmid#212891PurposeExpresses the Streptomyces griseolus LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP24
Plasmid#212857PurposeExpresses the Streptomyces cyslabdanicus LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP56
Plasmid#212889PurposeExpresses the Rhizobium meliloti LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP57
Plasmid#212890PurposeExpresses the Mesorhizobium japonicum LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP59
Plasmid#212892PurposeExpresses the Priestia megaterium LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP60
Plasmid#212893PurposeExpresses the Kitasatospora griseola LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP63
Plasmid#212896PurposeExpresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP21
Plasmid#212854PurposeExpresses the Nonomuraea jiangxiensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP16
Plasmid#212849PurposeExpresses the Actinomadura pelletieri LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP10
Plasmid#212843PurposeExpresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP27
Plasmid#212860PurposeExpresses the Kitasatospora sp. CB02891 LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP33
Plasmid#212866PurposeExpresses the Streptomyces platensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP22
Plasmid#212855PurposeExpresses the Scytonema sp. HK-05 LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP28
Plasmid#212861PurposeExpresses the Micromonospora humi LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP52
Plasmid#212885PurposeExpresses the Herbihabitans rhizosphaerae LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP39
Plasmid#212872PurposeExpresses the Saccharothrix texasensis LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP25
Plasmid#212858PurposeExpresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP04
Plasmid#212837PurposeExpresses the Rhizobium grahamii LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP02
Plasmid#212835PurposeExpresses the Sinorhizobium fredii LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP06
Plasmid#212839PurposeExpresses the Rhizobium acidisoli LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
ExpressionBacterialPromoterPcymRCAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBad-CAts TAG97
Plasmid#105668Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 97PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-AviTag-ALFAtag-eGFP
Plasmid#195854PurposeBacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.DepositorInsert6xHis-AviTag-ALFAtag-eGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasX1
Plasmid#87685Purposep15A plasmid expressing CasX locus from DeltaproteobacteriaDepositorInsertCasX1
ExpressionBacterialPromoterNoneAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_gfp
Plasmid#194154PurposeGreen Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
ExpressionBacterialPromoterPoxyR, PoxySAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTCF156 HLA-A*02:01
Plasmid#180456PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
ExpressionBacterialPromoterpj23119Available SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
TagsN-terminal His6 tagExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11-scarREF
Plasmid#221184PurposecdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)DepositorInsertscdGreen2
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen2) and PQ5 (cumate-inducible)Available SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-152
Plasmid#118534PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HC-M
Plasmid#173481PurposeAn rrnB P1-based GFP ATP reporter in E. coliDepositorInsertGFP-mut2
UseSynthetic BiologyTagsssrA degradation tagExpressionBacterialPromoterrrnB P1Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a 6xHis PreScission SARS-CoV-2 nsp13
Plasmid#159390PurposeExpresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.DepositorInsertN-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
TagsHis6 tagExpressionBacterialPromoterT7Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826-BB0323-mCherryBb
Plasmid#118244PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoterDepositorInsertBB0323-mCherry translational fusion under the control of the bb0826 promoter
TagsmCherryExpressionBacterialAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Snf7
Plasmid#21492DepositorAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXW109Hg(RS-RinA-E11)2
Plasmid#123151PurposeMercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLE, PrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-MCM4/6
Plasmid#116951PurposeExpresses human MCM4 and human MCM6DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
TagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only