Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,115 results

Showing: 2401 - 2430 of 32115 results

  1. pDule2-pCNF

    Plasmid
    #85495
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85495-plasmid-map-sequence-id-172459

    Enlarge

  9. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available Since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

    Enlarge

  13. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

    Enlarge

  15. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

    Enlarge

  19. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

    Enlarge

  27. pVP91A-pcaHG

    Plasmid
    #113766
    Purpose
    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.
    Depositor
    John Lipscomb
    Article
    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11
    Inserts
    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    Tags
    Met-6xHis
    Expression
    Bacterial
    Promoter
    T5 promoter
    Available Since
    Aug. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    113766-plasmid-map-sequence-id-218346

    Enlarge

  30. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

    Enlarge

Showing: 2401 - 2430 of 32115 results