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Plasmid#53479DepositorInsertRabx6 (RabX6 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB
Plasmid#52889Purpose"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expressionDepositorInsertGal4 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVTagsExpressionInsectMutationPromoterP10 and polyhedrinAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2
Plasmid#185449PurposeCoexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV siteDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9PromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 INI1
Plasmid#1953DepositorInsertINI1 (SMARCB1 Human)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-CKS1
Plasmid#177013PurposeGenerate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEVDepositorInsertCKS1 (CKS1B Human, Synthetic)
UseTagsPolyhistidine-TEVExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV5B+longBRD4
Plasmid#157792PurposeExpression of long isoform of BRD4DepositorInsertlong BRD4 (BRD4 Human)
UseTagsHis6ExpressionInsectMutationPromoterpolyhedronAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-QS
Plasmid#24366DepositorInsertQS
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBIG2ab
Plasmid#80616PurposepBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual LIC cloning vector (5A)
Plasmid#30121DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-WT
Plasmid#111572PurposeExpresses full length PARP1 in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
UseTagsC-terminal 6xHis and C-terminal FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP
Plasmid#32149DepositorInsertFRT> STOP STOP FRT>
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-GFP
Plasmid#171571PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cellsDepositorInsertNb127D01-GFP (CXCR2 Human)
UseTagsGFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2
Plasmid#80275PurposeGAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
DDB1
Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
doraWT_rescue_construct
Plasmid#190609PurposePuromycin-selectable expression of Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWALIUM10-V5-TurboID-GW
Plasmid#196285PurposeGateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic fliesDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC2-10XUAS-IVS-mCD8::GFP
Plasmid#26214DepositorInsertmCD8::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFB-EPCP1
Plasmid#226783PurposeExpression of Z-tagged LRRK2 RCKW using BaculovirusesDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsHis-Z-TEVExpressionInsectMutationAmino acids deleted 1-1326PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-SPARC2-D-LexA::p65
Plasmid#133562PurposeSwap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locusDepositorInsertLexA::p65
UseCRISPRTagsExpressionInsectMutationPromoter20X UASAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2
Plasmid#125161PurposeExpresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEZH2 (EZH2 Human)
UseBaculovirus expression vectorTagsHis-MBPExpressionInsectMutationPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprTagsExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-LexAGAD)
Plasmid#194769PurposeTo insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.DepositorInsertT2A-LexAGAD
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
UseTagshuman H2BExpressionInsectMutationPromoter10xUASAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE6n
Plasmid#17460Purposemodified pENTR vector for N-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLIB-GST-3C-CDK1
Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
UseTagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only