4,932 results

1. pAW-NbVHH05-mCherry

   Plasmid

   #171572

   Purpose

   Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-mCherry (UBE2J1 Human)
   

   Tags

   mCherry

   Expression

   Insect

   Promoter

   fly actin5C promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1 Flag BRG1

   Plasmid

   #1957

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BRG1 (SMARCA4 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

3. 53 pLIB_MBP

   Plasmid

   #160301

   Purpose

   N-term 3C cleavable MBP tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis and MBP

   Expression

   Insect

   Promoter

   polyhydrin

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIB_GST-TEV-SRC(WT)

   Plasmid

   #223742

   Purpose

   Expression of recombinant protein for purification, codon optimized for Insect cells

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Tags

   GST-TEV

   Expression

   Insect

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFast-Flag-hRad9

   Plasmid

   #222468

   Purpose

   Expresses hRad9 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad9 (RAD9A Human)
   

   Tags

   Flag epitope

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBOT563

   Plasmid

   #40864

   Depositor

   Harold Gainer

   Article

   Fields et al PLoS One. 2012;7(2):e32085. Epub 2012 Fe

   Insert

   Oxytocin promoter
   

   Use

   AAV

   Expression

   Bacterial and Insect

   Promoter

   mouse oxytocin promoter

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBOH-MHL.RBBP4

   Plasmid

   #125173

   Purpose

   Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Histone-binding protein RBBP4 (RBBP4 Human)
   

   Tags

   His-TEV site

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHsp83-NLS-HA-2xPCP-2xGFP

   Plasmid

   #71243

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   2xPCP-2xGFP
   

   Use

   P element-mediated germline transformation

   Tags

   EGFP

   Expression

   Insect

   Promoter

   Hsp83

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBac-DsRed-ORCO_9kbProm-QF2

   Plasmid

   #104877

   Purpose

   Anopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoes

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   AgORCO-QF2
   

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCoofy51

    Plasmid

    #122005

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    TwinStrep

    Expression

    Insect

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCoofy60

    Plasmid

    #122012

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

    Depositor

    Peggy Stolt-Bergner, Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-eGFP

    Expression

    Insect

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-Hrp48-ADARcd-V5

    Plasmid

    #81172

    Purpose

    identification of cell-specific targets of RNA binding proteins

    Depositor

    Michael Rosbash

    Article

    McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

    Insert

    Hrp48-ADARcd fusion (Hrb27C Fly)
    

    Expression

    Insect

    Promoter

    Drosophila metallothionein promoter

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pActP-GCaMP6m

    Plasmid

    #64714

    Purpose

    visualizing calcium in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    GCaMP6m
    

    Expression

    Insect

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

    Plasmid

    #163928

    Purpose

    Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAST-attB-yki:V5-S168A

    Plasmid

    #85617

    Purpose

    Expresses V5 tagged mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S168A
    

    Tags

    V5

    Expression

    Insect

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFast-hRad1

    Plasmid

    #222471

    Purpose

    Expresses hRad1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hRad1 (RAD1 Human)
    

    Expression

    Insect

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCaSpeR4-tubulin-QF#7

    Plasmid

    #46127

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBPGUw-HACK-G4>QF2

    Plasmid

    #80277

    Purpose

    GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

    Depositor

    Christopher Potter

    Article

    Lin et al Genetics. 2016 Jun 22. pii: genetics.116

    Insert

    QF2
    

    Expression

    Insect

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Tags

    His6

    Expression

    Insect

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST-HemmarR

    Plasmid

    #31222

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdTom (CD4 Human, Fly, Aequorea victoria)
    

    Tags

    CD4 and tdTomato

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTriExEKAR2G2

    Plasmid

    #40175

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
    

    Tags

    SpyTag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSPLp10

    Plasmid

    #170462

    Purpose

    EarlyBac insect cell expression system

    Depositor

    Hiro Furukawa

    Article

    Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

    Insert

    New vector
    

    Expression

    Insect

    Promoter

    dHsp70

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. Ubiq-Cas9.874W

    Plasmid

    #112686

    Purpose

    Express hSpCas9 under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    Ubiquitin-63E promoter

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

    Plasmid

    #130976

    Purpose

    Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    Hook3-SNAPf (HOOK3 Human)
    

    Tags

    8His-ZZ-LTLT and SNAPf

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ppBAalbNixE1E2 PUb-GFP

    Plasmid

    #173667

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    EGFP
    

    Expression

    Insect

    Mutation

    Nix isoforms with only exons 1, 2 separated by in…

    Promoter

    Aedes aegypti Polyubiquitin and cloned Nix promot…

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac-hDicer

    Plasmid

    #89144

    Purpose

    Expression of hDicer in Sf9 Cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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31. pAcRP67A prM/E YFV

    Plasmid

    #52883

    Purpose

    expression of Yellow Fever Virus VLPs in baculovirus

    Depositor

    Yorgo Modis

    Insert

    prM/E
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

32. Strep-His-Tev-hCGN

    Plasmid

    #236512

    Purpose

    For expression of Cingulin (human) in insect cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGN (CGN Human)
    

    Tags

    2xStrep-10xHis-Tev

    Expression

    Insect

    Mutation

    WT

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pADC10-SETXhel

    Plasmid

    #193056

    Purpose

    Baculovirus expression of human Senataxin helicase domain for protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Senataxin (SETX Human)
    

    Tags

    Flag

    Expression

    Insect and Mammalian

    Mutation

    codon optimized for insect cell expression

    Promoter

    hybrid p10 (insect) and CMV (human) expression

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAEL005635-Cas9

    Plasmid

    #100608

    Purpose

    The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL005635-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL005635 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 47 pLIB_GST

    Plasmid

    #160272

    Purpose

    N-term 3C cleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GST

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. OA-986B

    Plasmid

    #124999

    Purpose

    Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VPR
    

    Use

    Piggyback

    Expression

    Insect

    Promoter

    Ubiquitin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFastBac1-Flag-Timeless

    Plasmid

    #23119

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Timeless (TIMELESS Human)
    

    Tags

    Flag

    Expression

    Insect

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

39. GFP-Rab32

    Plasmid

    #31744

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab32 (Rab32 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pHD-SPARC2-S-LexA::p65

    Plasmid

    #133564

    Purpose

    Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge