4,908 results

1. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 78H05-FRT100-STOP-FRT100-p65.AD

   Plasmid

   #232828

   Purpose

   Enhancer 78H05 driven p65.AD, gated by Sparse.FRT100

   Depositor

   Liqun Luo

   Article

   Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

   Inserts

   FRT100-STOP-FRT100
   p65.AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-Sparse.FRT10-p65.AD

   Plasmid

   #232830

   Purpose

   Enhancer-free Sparse.FRT10 p65.AD with ccdB for Gateway

   Depositor

   Liqun Luo

   Article

   Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

   Inserts

   FRT10-STOP-FRT10
   p65.AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGxRF3

   Plasmid

   #138384

   Purpose

   T2A-GAL4 knock-in cassette

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-GAL4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p2.0VPI.EGFP

   Plasmid

   #40868

   Depositor

   Harold Gainer

   Article

   Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

   Insert

   mouse vasopressin promoter
   

   Use

   AAV

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   mouse vasopressin

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSPLp10

   Plasmid

   #170462

   Purpose

   EarlyBac insect cell expression system

   Depositor

   Hiro Furukawa

   Article

   Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

   Insert

   New vector
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dHsp70

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

   Plasmid

   #231733

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CTR9 (CTR9 Human)
   CDC73 (CDC73 Human)
   PAF1 (PAF1 Human)
   LEO1 (LEO1 Human)
   WDR61 (SKIC8 Human)
   

   Use

   Tags

   3C-Strep(II) and Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-His10TEV

   Plasmid

   #159427

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Use

   Tags

   TEV-protease-cleavable His10-tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFast-hRad1

    Plasmid

    #222471

    Purpose

    Expresses hRad1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hRad1 (RAD1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBOH-MHL.RBBP4

    Plasmid

    #125173

    Purpose

    Expresses human RBBP4 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Histone-binding protein RBBP4 (RBBP4 Human)
    

    Use

    Tags

    His-TEV site

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162388

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p{CFD4-3xP3::DsRed}

    Plasmid

    #86864

    Purpose

    Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC5.1-bgal-V5His

    Plasmid

    #136238

    Purpose

    Protein expression of Beta-galactosidase with C-terminal V5 and His tags

    Depositor

    Aziz Sancar, Steven Reppert

    Article

    Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

    Insert

    Beta-galactosidase
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFD5-frame_selector_0,1,2

    Plasmid

    #131152

    Purpose

    Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    CRISPaint frame selector sgRNAs 0,1,2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6:3

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Use

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLfa-PUb-EGFP-3'UTR#13

    Plasmid

    #176338

    Purpose

    The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

    Depositor

    Zach Adelman

    Article

    Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

    Insert

    3'UTR#13
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIZ-Flag6His-BmAgo3

    Plasmid

    #50559

    Purpose

    Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    BmAgo3
    

    Use

    Tags

    6xHIS and Flag

    Expression

    Insect

    Mutation

    Promoter

    OpIE2

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJFRC164-21XUAS-KDRT>-dSTOP-KDRT>-myr::RFP

    Plasmid

    #32141

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    KDRT> STOP STOP KDRT>
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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