4,932 results

1. pOPINVH

   Plasmid

   #26041

   Depositor

   Ray Owens

   Article

   Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

   Insert

   u-LacZ-KHHHHHH
   

   Tags

   KHHHHHH and u-phosphatase secretion leader

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-Aurora B-GFP

   Plasmid

   #24285

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Aurora B Kinase (aurB Fly)
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

   Plasmid

   #217511

   Purpose

   KD Recombinase dependent conditional smGdP-10XHA cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
   

   Tags

   smGdP-10XHA

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMycDam

   Plasmid

   #59218

   Purpose

   Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam and Myc

   Expression

   Insect

   Promoter

   Drosophila heat-shock promoter

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJFRC7-JEDI-2P

   Plasmid

   #179461

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P under the promoter hsp70 for Drosophila (insect) expression

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct:dCas9

   Plasmid

   #78903

   Purpose

   Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

   Plasmid

   #48296

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP

   Expression

   Insect

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJFRC14-10XUAS-IVS-GFP-WPRE

   Plasmid

   #26223

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   GFP
   

   Expression

   Insect

   Available Since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1 Flag BAF155 b

   Plasmid

   #1954

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BAF155 (SMARCC1 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pUASattB-ERK-SPARK

    Plasmid

    #106923

    Purpose

    Express ERK-SPARK, an activity reporter of ERK in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    ERK-SPARK
    

    Expression

    Insect

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. QUAS-jGCaMP7s-T2A-tdTomato

    Plasmid

    #200010

    Purpose

    QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    3xP3-ECFP-jGCaMP7s-td-Tomato
    

    Expression

    Insect

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFB1.HMBP.PrS.EED

    Plasmid

    #125163

    Purpose

    Expresses human EED in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein EED (EED Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFD3-frame_selector_0

    Plasmid

    #127553

    Purpose

    Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sgRNA frame 0
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC-QF

    Plasmid

    #24338

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QF
    

    Expression

    Insect

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. U6c

    Plasmid

    #117223

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6c promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRB14

    Plasmid

    #52522

    Purpose

    expresses a myc-tagged version of hCas9 in Drosophila

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    S. pyogenes cas9 with humanized codon bias
    

    Tags

    myc

    Expression

    Insect

    Promoter

    tubulin

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUASp YFP Rab5 WT

    Plasmid

    #53468

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab05 (Rab5 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAC-GAL80

    Plasmid

    #24346

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL80 (GAL80 )
    

    Expression

    Insect

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-Dual_FluA/H7N9

    Plasmid

    #229860

    Purpose

    pFastBac Dual vector for co-expression of FluPolA/H7N9 polymerase heterotrimer (PA/PB1/PB2)

    Depositor

    Stephen Cusack

    Article

    Kouba et al Cell Rep. 2023 Jan 31;42(1):111901. doi:

    Inserts

    PA_H7N9-FluA
    PB1_H7N9-FluA
    PB2_H7N9-FluA
    

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. doraWT_rescue_construct

    Plasmid

    #190609

    Purpose

    Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Expression

    Insect

    Promoter

    Actin-5c

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p2Bac.XPC

    Plasmid

    #39204

    Depositor

    Aziz Sancar

    Article

    Reardon et al J Biol Chem. 1996 Aug 9;271(32):19451-6.

    Insert

    XPC (XPC Human)
    

    Expression

    Insect

    Mutation

    Q475K polymorphism

    Promoter

    Pp10

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pQUASp

    Plasmid

    #46162

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. piB-GFP

    Plasmid

    #13844

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    TATA-GFP-SV40
    

    Expression

    Insect

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAWG-dCas9-VPR

    Plasmid

    #63802

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Insect

    Promoter

    act5c

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

    Plasmid

    #55217

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Available Since

    July 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pScarlessHD-C-3xVHH05-DsRed

    Plasmid

    #171580

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Expression

    Insect

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Tags

    His-MBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UAS-myr::4xSNAPf

    Plasmid

    #87637

    Purpose

    For creating drosophila transgenics expressing UAS-myr4x::SNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Expression

    Insect

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NHR1+2-Flag pBacPAK8-3

    Plasmid

    #17324

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Neuralized-like (Neurl1a Mouse)
    

    Tags

    FLAG

    Expression

    Insect

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pLib6.4B-Consensus

    Plasmid

    #176678

    Purpose

    Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriEx-mPA-GFP-PA-Rac1

    Plasmid

    #22022

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His and mPA-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pMT-EGFP-Actin5c

    Plasmid

    #15312

    Depositor

    Ron Vale

    Article

    Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

    Insert

    Actin5c (Act5C Fly)
    

    Tags

    EGFP and His

    Expression

    Insect

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pOPINHALO7

    Plasmid

    #41117

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218864

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPaint-T2A-sfGFP-3xP3-RFP

    Plasmid

    #127557

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pActPL-Gal4DBD

    Plasmid

    #15304

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    ZIP- Gal4DBD (GAL4 )
    

    Expression

    Insect

    Available Since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. U6b

    Plasmid

    #117222

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    HR U6b promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ppBAalbNixE1E2 OpIE2-GFP

    Plasmid

    #173505

    Purpose

    transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Inserts

    Nix masculinisation gene
    EGFP
    

    Expression

    Insect

    Mutation

    Nix isoforms with exons 1, 2 separated by intron 1

    Promoter

    OpIE2 and own

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge