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pJFRC5-5XUAS-IVS-mCD8::GFP

Plasmid

#26218

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Expression

Insect

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

Plasmid

#86453

Purpose

UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

Depositor

Gregor Bucher

Insert

3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40

Expression

Insect

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

Cell Division Cycle 42 (CDC42 Human)

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR R4-vas2-integrase-R3

Plasmid

#62299

Purpose

phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

phageC31 integrase under control of vasa promoter and terminator

Tags

HA and SV40 NLS

Expression

Insect

Promoter

vasa

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmec-4::Chrimson::SL2::mCherry::unc-54

Plasmid

#107745

Purpose

Express Chrimson and mCherry in mechanosensory neurons.

Depositor

Andrew Leifer

Article

Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

Inserts

Chrimson
mCherry

Expression

Insect and Worm

Promoter

mec-4

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pIHEU-MCS

Plasmid

#58375

Purpose

UAS vector to express gene of interest in Drosophila

Depositor

Chun Han

Article

Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

Type

Empty backbone

Expression

Insect

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF155/SMARCC1-His

Plasmid

#177863

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCC1 (SMARCC1 Human)

Tags

His and His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCoofy28

Plasmid

#44004

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-GST

Expression

Insect

Promoter

PPH

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJFRC2-10XUAS-IVS-mCD8::GFP

Plasmid

#26214

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

mCD8::GFP

Expression

Insect

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pUASp-GBP-Tbro

Plasmid

#179995

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Tags

Trbo

Expression

Insect

Promoter

UAS-Gal4

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pExpreS2-2

Plasmid

#175362

Purpose

G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1 human full-length CENP-E-mNeon-6xHis

Plasmid

#180484

Purpose

pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

Depositor

Julie Welburn

Article

Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

Insert

CENP-E (CENPE Synthetic, human, Human)

Tags

mNeon-6xHis

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(rhesus)

Plasmid

#79033

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(rhesus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Tags

3xFLAG

Expression

Insect

Promoter

10XUAS

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMB.BIG1a.PRC2

Plasmid

#234581

Purpose

Expression of EZH2, MBP-SUZ12, EED, RBBP4

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Insect

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMB.PRC2.AEBP2

Plasmid

#234580

Purpose

Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3]

Depositor

Chen Davidovich

Article

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4, AEBP2 [Iso 3] (EZH2, SUZ12, RBBP4, EED, AEBP2 Human)

Expression

Insect

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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