4,908 results

1. pUASTattB-QS

   Plasmid

   #24366

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QS
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. p130Cas Y15F

   Plasmid

   #186154

   Purpose

   For expressing recombinant p130Cas in insect cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Use

   Tags

   His6 (Tev)

   Expression

   Insect

   Mutation

   15Y residues mutated to Alanine

   Promoter

   polyhedrin

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFB-EPCP5

   Plasmid

   #228879

   Purpose

   Expression of Z-tagged LRRK2 KW using Baculoviruses

   Depositor

   Sebastian Mathea

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   Leucine-rich repeat kinase 2 (LRRK2 Human)
   

   Use

   Tags

   His-Z-TEV

   Expression

   Insect

   Mutation

   Amino acids deleted 1-1846

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDyn1

   Plasmid

   #64067

   Purpose

   Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

   Depositor

   Andrew Carter

   Article

   Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

   Insert

   DYNC1H1 (DYNC1H1 Human)
   

   Use

   Cre/Lox

   Tags

   His-ZZ-TCS-SNAPf

   Expression

   Insect

   Mutation

   Codon optimized for Sf9

   Promoter

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

   Plasmid

   #190928

   Purpose

   Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Tags

   Strep-TEV-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Use

   Tags

   Strep Sumo

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHD-SPARC2-S-LexA::p65

   Plasmid

   #133564

   Purpose

   Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCoofy11

   Plasmid

   #44002

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His10

   Expression

   Insect

   Mutation

   Promoter

   hr5

   Available since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest-795

   Plasmid

   #161881

   Purpose

   Standard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell system

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Tags

   MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ClCry4 PHR

    Plasmid

    #160552

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 HR
    

    Use

    Tags

    Hexa his

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC5.1-dCry-V5His

    Plasmid

    #136237

    Purpose

    Protein expression of D. melanogaster (Dm) cryptochrome with C-terminal V5 and His tags

    Depositor

    Steven Reppert, Aziz Sancar

    Article

    Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-U63-QtgRNA2.1-BR

    Plasmid

    #170513

    Purpose

    gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid

    #35023

    Depositor

    Jing-W Wang

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    VP16 and mLexA

    Expression

    Insect

    Mutation

    deletion of amino acids 590-716

    Promoter

    UAS

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-U63-gRNA2.1

    Plasmid

    #170512

    Purpose

    gRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6:3

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBPGAL4.1Uw

    Plasmid

    #26226

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. OA-1053B

    Plasmid

    #184007

    Purpose

    Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNAhh1 - U6c:sgRNAhh2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFv-UAS3

    Plasmid

    #138399

    Purpose

    10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKL

    Plasmid

    #110741

    Purpose

    Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac_His-ZZ-TEV-Lis1

    Plasmid

    #132539

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tag

    Depositor

    Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker_TEV, and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAV5B+longBRD4

    Plasmid

    #157792

    Purpose

    Expression of long isoform of BRD4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    long BRD4 (BRD4 Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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