5,038 results
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Plasmid#43905DepositorAvailable SinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only
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bTub-GFP.766A
Plasmid#112695Purposeexpressed GFP in testes, under bTub promoterDepositorInsertbTub-GFP-SV40
ExpressionInsectPromoterbTubAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969Available SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
Tags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716Available SinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW-EGFP-4xlet-7 (pAW-AS27)
Plasmid#40834PurposemiRNA sensor used in Drosophila cell linesDepositorInsertFour target sites perfectly complementary to let-7
UseMirna reporterTagsEGFPExpressionInsectPromoterActin5CAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-p65AD::CaM
Plasmid#64715Purposegenerating transgenic TRIC fliesDepositorInsertp65AD::CaM
ExpressionInsectAvailable SinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKM744
Plasmid#134225PurposeBaculovirus expression of CENP-T and CENP-WDepositorInserthis-CENP T + CENP W
ExpressionInsectAvailable SinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOEM-GED-mCherry
Plasmid#102781PurposeBaculovirus rescue vector, VSVGED pseudotype, mCherryDepositorInsertmCherry
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
RIPK2 (RIPK2A-c036)
Plasmid#110268PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CG10077-RA_B
Plasmid#145910PurposeInsect Expression of CG10077-RADepositorInsertCG10077-RA (CG10077 Fly)
ExpressionInsectMutationA23T, Q440R, S483L, I492T, inserted G810, H672Y, …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CG10077-RB_B
Plasmid#145911PurposeInsect Expression of CG10077-RBDepositorAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
AT5G48740 O3_pECIA2
Plasmid#114963PurposeBait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.DepositorInsertAT5G48740 (AT5G48740 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT5G59650 O4_pECIA2
Plasmid#114964PurposeBait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.DepositorInsertAT5G59650 (AT5G59650 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CJ53 (cg5166a/c utr)
Plasmid#17781DepositorInsertcg5166a/c utr fragment
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectAvailable SinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
CJ54 (cg5166a/c utr mut)
Plasmid#17782DepositorInsertcg5166a/c utr fragment mut
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationmutant versionAvailable SinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-SAK
Plasmid#31755DepositorAvailable SinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A
Plasmid#69792PurposePebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble - Drosophila guanine nucleotide exchange factor
UseP element-based puast vector for gal4-regulated e…TagsHA tagExpressionInsectMutationEncodes Pbl (NP_729306.1) lacking last 9 amino ac…Promoterhsp70 promoterAvailable SinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
HBM-mVEGF-C-HisTag-pENTR
Plasmid#64066PurposePlasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.DepositorAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-Sas4
Plasmid#31756DepositorAvailable SinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab5 DN
Plasmid#44823DepositorInsertRab5 (Rab5 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS43N (dominant negative)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCoofy59
Plasmid#122011PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage siteDepositorTypeEmpty backboneTagsTrxA-His6ExpressionInsectAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa
Plasmid#86451PurposeGal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)DepositorInsertHsp_p-GAL4Delta-SV40;attp
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3H
Plasmid#49050PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionInsectPromoterpolH (polyhedrin)Available SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFBXNH3
Plasmid#49049PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionInsectPromoterpolH (polyhedrin)Available SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAGHis10pFastBacHTA/Serca1
Plasmid#74401Purposeexpression of SERCA in insect cellsDepositorInsertSERCA1 (ATP2A1 rabbit)
Tags10His and FLAGExpressionInsectMutationNcoI site destroyed (silent) T898PromoterpolyhedrinAvailable SinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb AaegOr4-E
Plasmid#59796PurposeUAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele EDepositorInsertAedes aegypti Or4 cDNA allele E
ExpressionInsectAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 cyto Grx1-roGFP2
Plasmid#64999PurposeExpression of cytosolic Grx1-roGFP2 under the alphaTub84B promoterDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMSH2_G674A
Plasmid#134874PurposeExpression human MSH2 G674 mutant protein using baculovirusDepositorInserthuman MSH2_G674A (MSH2 Human)
ExpressionInsectMutationChanged Glycine to Alanine at aa#674Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
TagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable SinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab4 CA
Plasmid#53466DepositorInsertRab04
TagsYFPExpressionInsectMutationQ67LPromoterGAL4Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato mouse Rab35 WT
Plasmid#53514DepositorAvailable SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-FOXO 3A
Plasmid#52983Purposeinsect expression of FOXO triple mutantDepositorInsertFOXO (foxo Fly)
Tags3x HAExpressionInsectMutation3A, triple mutant, T44A S190A S259APromoterHsp70Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B-Cquin_596
Plasmid#176669PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSR
Plasmid#165838Purposeblasticidin S resistance markerDepositorInsertbsr
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3GFP
Plasmid#182203Purpose1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGRY
Plasmid#61599PurposeTransformation vector pBGRYDepositorTypeEmpty backboneUseDrosophila transformation vectorExpressionInsectAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBC2FG
Plasmid#31281DepositorAvailable SinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>shibire (ts1)
Plasmid#24363DepositorInsertShibire (ts1) (shi Fly)
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMT-HA-STX4
Plasmid#89311PurposeExpresses HA tagged syntaxin 4 in Drosophila cellsDepositorAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-RFPnls-PQRv2-mCD8:GFP
Plasmid#73974PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.DepositorInsertssfGFP
RFP
TagsmCD8 and nlsExpressionInsectPromoter20XUAS-HSAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-STIL
Plasmid#80265Purposebaculovirus creation plasmid for GST-STILDepositorAvailable SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBY2887
Plasmid#23221DepositorInsertSnAvi
ExpressionBacterial, Insect, and Mamm…Available SinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab11 DN
Plasmid#44831DepositorInsertRab11 (Rab11 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationS25N (dominant negative)Available SinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP2 CC+CKK
Plasmid#59039Purposefor baculovirus expression of CAMSAP2 CC+CKK in Sf9 cellsDepositorAvailable SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
DYNLL1
Plasmid#132530PurposeHuman LC8-1, codon optimised for Sf9 expression.DepositorAvailable SinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
WDR34
Plasmid#132526PurposeHuman WDR34, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
TCTEX1D2
Plasmid#132529PurposeHuman TCTEC-1D2, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
DYNC2LI1
Plasmid#132527PurposeHuman LIC3, codon optimised for Sf9 expression.DepositorAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only