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5,017 results

Showing: 1651 - 1700 of 5017 results

  11. pUAST-HA, Pbl-A

    Plasmid
    #69792
    Purpose
    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.
    Depositor
    Arno Muller
    Article
    van Impel et al Development. 2009 Mar;136(5):813-22. doi
    Insert
    Pebble - Drosophila guanine nucleotide exchange factor
    Use
    P element-based puast vector for gal4-regulated e…
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…
    Promoter
    hsp70 promoter
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid
    #86451
    Purpose
    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Hsp_p-GAL4Delta-SV40;attp
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86451-plasmid-map-sequence-id-171105

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  15. pOCC254

    Plasmid
    #118889
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mCherry, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118889-plasmid-map-sequence-id-232286

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  17. FLAGHis10pFastBacHTA/Serca1

    Plasmid
    #74401
    Purpose
    expression of SERCA in insect cells
    Depositor
    Robert Nakamoto
    Insert
    SERCA1 (ATP2A1 rabbit)
    Tags
    10His and FLAG
    Expression
    Insect
    Mutation
    NcoI site destroyed (silent) T898
    Promoter
    polyhedrin
    Available Since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. pUAST-FOXO 3A

    Plasmid
    #52983
    Purpose
    insect expression of FOXO triple mutant
    Depositor
    Marc Montminy, Biao Wang
    Insert
    FOXO (foxo Fly)
    Tags
    3x HA
    Expression
    Insect
    Mutation
    3A, triple mutant, T44A S190A S259A
    Promoter
    Hsp70
    Available Since
    May 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  27. pMT-HA-STX4

    Plasmid
    #89311
    Purpose
    Expresses HA tagged syntaxin 4 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX4 (Syx4 Fly)
    Tags
    HA tag
    Expression
    Insect
    Promoter
    P-MT (Metallothionein)
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89311-plasmid-map-sequence-id-190169

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  42. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    John Diffley
    Article
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169164-plasmid-map-sequence-id-333982

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  46. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid
    #86455
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86455-plasmid-map-sequence-id-171155

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  50. DPP7 (3JYH)

    Plasmid
    #25354
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    dpp07.029.492 (DPP7 Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1651 - 1700 of 5017 results