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82,463 results

Showing: 361 - 380 of 82463 results

  1. pdCas9-humanized

    Plasmid
    #44246
    Purpose
    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown
    Depositor
    Stanley Qi
    Publication
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dead Cas9 with 3X NLS
    Use
    CRISPR and Retroviral; Vector
    Tags
    3x NLS
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    MSCV LTR promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17 Articles
    44246-plasmid-map-sequence-id-60247

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  3. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV5, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    372 Articles
    20298-plasmid-map-sequence-id-191904

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  7. pEF.myc.ER-E2-Crimson

    Plasmid
    #38770
    Purpose
    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)
    Depositor
    Benjamin Glick
    Publication
    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    E2-Crimson
    Tags
    ER retention signal (KDEL), ER signal sequence (M…
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    38770-plasmid-map-sequence-id-200379

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  13. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid
    #119816
    Purpose
    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells
    Depositor
    Ethan Abel
    Publication
    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:
    Inserts
    Luciferase
    Puromycin
    Use
    Lentiviral and Luciferase
    Tags
    EGFP and V5
    Expression
    Mammalian
    Mutation
    Stop codon removed in the frame of the V5 frame v…
    Promoter
    CMV/TO and Human Phosphoglycerate kinase
    Available since
    Jan. 2, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    15 Articles
    119816-plasmid-map-sequence-id-372950

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  17. pMSCV-LTR-dCas9-VP64-BFP

    Plasmid
    #46912
    Purpose
    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP
    Depositor
    Stanley Qi, Jonathan Weissman
    Publication
    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008
    Inserts
    dCas9-VP64-BFP fusion
    Puromycin resistance
    Use
    CRISPR and Retroviral
    Tags
    3xNLS, BFP, and VP64 domain
    Expression
    Mammalian
    Promoter
    LTR and PGK
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11 Articles
    46912-plasmid-map-sequence-id-66610

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  20. Cre Reporter

    Plasmid
    #62732
    Purpose
    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).
    Depositor
    Niels Geijsen
    Publication
    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1
    Insert
    LoxP-DsRED-STOP-LoxP-eGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    LoxP sites
    Expression
    Mammalian
    Promoter
    EF1-Alpha
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    62732-plasmid-map-sequence-id-204219

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Showing: 361 - 380 of 82463 results