78,019 results

1. pLV-REPORT (PGK) 12xHRE-NanoLuc

   Plasmid

   #208575

   Purpose

   hypoxic response element driven NanoLuc Lentiviral reporter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   NanoLuciferase
   d2eGFP
   12xHRE
   

   Use

   Lentiviral, Luciferase, and Synthetic Biology

   Tags

   3x SV40 NLS and PEST

   Expression

   Mammalian

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nup133_mEGFP(-8a)

   Plasmid

   #163417

   Purpose

   Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)

   Depositor

   Sanford Simon

   Insert

   Nup133 (NUP133 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. VPS13B^Halo

   Plasmid

   #224584

   Purpose

   codon optimized Halo tagged VPS13B for expression in mammalian cells

   Depositor

   Pietro De Camilli

   Article

   Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

   Insert

   VPS13B^Halo (VPS13B Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIC162

   Plasmid

   #63070

   Purpose

   Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   Nuf2 (NUF2 Human)
   

   Use

   Retroviral

   Tags

   EGFP and TEV-S

   Expression

   Mammalian

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. PGKneotpAlox2

   Plasmid

   #13444

   Depositor

   Philippe Soriano

   Article

   Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

   Insert

   PGKneotpAlox2
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_Lifeact_HaloTag9

   Plasmid

   #175545

   Purpose

   Expression of HaloTag9 at the actin filaments of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Lifeact-HaloTag9
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_PA-Cre

   Plasmid

   #122960

   Purpose

   Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

   Insert

   pcDNA3.1_PA-Cre
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PPP1CA

   Plasmid

   #155843

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PPP1CA (PPP1CA Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT1 KI gRNA1

   Plasmid

   #92312

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn-RiboL1-jGCaMP8s

    Plasmid

    #169247

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST26-PPP2CB-C-HA

    Plasmid

    #195179

    Purpose

    Vector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    PPP2CB (PPP2CB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. IronFistMUT.

    Plasmid

    #243008

    Purpose

    Iron binding deficient IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    hemerythrin-like-mNeonGreen (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    H15A, H57A, E58A, E61A, H126A, E130A

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Iron Insensitive Control Construct 2

    Plasmid

    #243007

    Purpose

    Iron Insensitive Control Construct under the same expression promoter used in IronFist

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    mNeonGreen
    T2A
    mCherry
    

    Use

    Gateway

    Expression

    Mammalian

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. IronFist

    Plasmid

    #243006

    Purpose

    Genetically encoded fluorescent iron reporter

    Depositor

    Roland Malli

    Article

    Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

    Inserts

    Hemerythrin-like (FBXL5 Human)
    T2A
    mCherry
    

    Use

    Gateway

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    human phosphoglycerate kinase (hPGK)

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. SBE-Luc

    Plasmid

    #16527

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    Smad binding element
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDN3.1(+)-CaNARi

    Plasmid

    #64848

    Purpose

    FRET-based calcineurin (CaN) activation biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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