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82,528 results

Showing: 5921 - 5940 of 82528 results

  1. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86612-plasmid-map-sequence-id-168849

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  5. mCyRFP1-C1

    Plasmid
    #84355
    Purpose
    Expresses mCyRFP1 fluorescent protein in an empty backbone
    Depositor
    Ryohei Yasuda
    Publication
    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCyRFP1
    Expression
    Mammalian
    Mutation
    A161K and N41A, reletive to CyRFP1
    Promoter
    CMV
    Available since
    Nov. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    84355-plasmid-map-sequence-id-160941

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  6. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid
    #74309
    Purpose
    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
    Depositor
    Ali Guler
    Publication
    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74309-plasmid-map-sequence-id-153122

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  12. pCITF-KCC2

    Plasmid
    #61404
    Purpose
    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence
    Depositor
    Franck Polleux
    Publication
    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KCC2 (SLC12A5 Human)
    Tags
    IRES-tdTomato-Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61404-plasmid-map-sequence-id-122754

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  14. GFP-BRDT

    Plasmid
    #65381
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRDT (BRDT Human)
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    65381-plasmid-map-sequence-id-245402

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  15. BiP-mCherry

    Plasmid
    #62233
    Purpose
    Hamster BiP/GRP78/HSPA5 fused to mCherry. Useful for studying BiP mobility and trafficking in live cells.
    Depositor
    Erik Snapp
    Publication
    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hamster BiP-mCherry-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62233-plasmid-map-sequence-id-314153

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  17. mCherry-MAPTau-C-10

    Plasmid
    #55077
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPTau (MAPT Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55077-plasmid-map-sequence-id-353040

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  20. mApple-Alpha-V-Integrin-25

    Plasmid
    #54866
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-V Integrin (ITGAV Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54866-plasmid-map-sequence-id-514621

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Showing: 5921 - 5940 of 82528 results