78,043 results

1. GG-EBNA-OSK2M2L1-PP

   Plasmid

   #102902

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OSK2M2L1-gRNAs-PGK-puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204189

   Purpose

   Doxycycline inducible lentiviral vector for human GATA6 overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   GATA6 (GATA6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-NES-ZapCV2

   Plasmid

   #222950

   Purpose

   Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2 (NES Synthetic)
   

   Tags

   ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX-MCS PGK promoter

   Plasmid

   #128044

   Purpose

   Lentiviral vector with PGK promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GPR87-DuET

   Plasmid

   #213293

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR87 (GPR87 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-FLAG-KD LKB1

   Plasmid

   #8591

   Depositor

   Lewis Cantley

   Article

   Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

   Insert

   LKB1 (STK11 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K78I: lysine 78 mutated to isoleucine; kinase dead

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.10 Dub3

   Plasmid

   #72684

   Purpose

   luciferase reporter with Dub3 promoter

   Depositor

   Domenico Maiorano

   Article

   van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

   Insert

   Dub3 promoter (Usp17le Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Dub3

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. opto-Rac1

   Plasmid

   #135396

   Purpose

   Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

   Insert

   opto-Rac1
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PCMV-intron myc Rab2 S20N

    Plasmid

    #46780

    Purpose

    expression of S20N Rab2a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB2 (RAB2A Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S20N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-hRXRα

    Plasmid

    #135910

    Purpose

    Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Insert

    Human retinoid X receptor- alpha (RXRA Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 8xNFAT-ZsG-hCD4

    Plasmid

    #162746

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GW1-Mito-pHRed

    Plasmid

    #31474

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    Cox8-tagged pHRed
    

    Tags

    4xCox8

    Expression

    Mammalian

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ZNRF3-SmBiT, BiBiT-Ready Vector

    Plasmid

    #237083

    Purpose

    Express ZNRF3-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ZNRF3 (ZNRF3 Human)
    

    Tags

    SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lenti-25xAARE-minP-EGFP

    Plasmid

    #159666

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xAARE-minP-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid

    #86613

    Purpose

    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge