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LbABE8e

Plasmid

#138504

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Publication

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Gateway Cloning

Expression

Mammalian

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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UAS-mCherry

Plasmid

#135457

Purpose

Mammalian mCherry reporter to read out FLARE and SPARK activation

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Tags

mCherry

Expression

Mammalian

Promoter

UAS

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pAAV-Syn-SomArchon

Plasmid

#126941

Purpose

Expression of SomArchon under Syn promoter

Depositor

Edward Boyden

Publication

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Synapsin

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Publication

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.4

Plasmid

#123123

Purpose

Human expression vector for deltaproteobacteria CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Publication

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Publication

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti CMVie-IRES-BlastR

Plasmid

#119863

Purpose

3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

Depositor

Ghassan Mouneimne

Publication

Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV immediate early

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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TNFR1-YFP

Plasmid

#111209

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNFRSF1A (TNFRSF1A Human)

Tags

EYFP

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dLight1.2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Publication

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLVX-HA-UBE3A-IRES-Puro

Plasmid

#107349

Purpose

Expresses HA tagged UBE3A in mammalian cells.

Depositor

Robert Sobol

Publication

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3A (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Publication

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-EGFP-HOTag3

Expression

Mammalian

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-P2A-K(AAG)20-P2A-RFP

Plasmid

#105689

Purpose

Double fluorescent stalling reporter K(AAG)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

P2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SaBE4

Plasmid

#100805

Purpose

Expresses SaBE4 in mammalian cells

Depositor

David Liu

Publication

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SaBE4

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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