81,074 results

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pAAV.hSyn.CaMPARI.WPRE.SV40

Plasmid

#100832

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Article

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Has Service

AAV1, AAV5, and AAV9

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-mPERK-FLAG-IP

Plasmid

#101793

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PERK

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Perk (Eif2ak3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-mPKR-FLAG-IG

Plasmid

#101792

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PKR

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Pkr (Eif2ak2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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CD8-mCherry-FRB

Plasmid

#100738

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

FRB and mCherry

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PHFF-sh-hBVRA

Plasmid

#100285

Purpose

Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

Depositor

Kazuhiro Aoki

Article

Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

Inserts

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA

Tags

FLAG, T7 and Mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

All genes are codon-optimized for expression in h…

Promoter

CAG promoter and H1 promoter

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TK1

Plasmid

#100544

Purpose

mammalian expression of thymidine kinase 1

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidine kinase 1 (TK1 Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Bnip3FL

Plasmid

#100796

Purpose

Mammalian expression of Bnip3 full length splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mus Bnip3 (Bnip3 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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FlagMBNL3

Plasmid

#96901

Purpose

expression of Flag-tagged human MBNL3

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human MBNL3 cDNA (MBNL3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-BoxB

Plasmid

#92004

Purpose

Expresses firefly luciferase with BoxB sequences in 3' UTR

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Firefly Luciferase

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ZNF217_pLX307

Plasmid

#98384

Purpose

Lentiviral expression of ZNF217

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ZNF217 (ZNF217 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-USP7

Plasmid

#99393

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

USP7 (USP7 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-RBM3

Plasmid

#87860

Purpose

Expresses human RBM3 coupled to GFP

Depositor

Maria Alvarado-Kristensson

Article

Rossello et al Heliyon. 2016 Sep 24;2(9):e00166. eColle

Insert

RNA binding motif protein 3 (RBM3 Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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PBMBP-eGFP

Plasmid

#40976

Depositor

Joseph Loturco

Article

Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

Insert

MBP promoter fragment (Mbp Mouse)

Expression

Mammalian

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B Q78L

Plasmid

#89448

Purpose

Expression of GFP-tagged human Rab27B Q78L in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B Q78L (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

Q78L

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-SpvB

Plasmid

#89455

Purpose

Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-GluA1-CIB

Plasmid

#89444

Purpose

Allows conditional recruitment of AMPA receptors to the postsynaptic membrane

Depositor

Matthew Kennedy

Article

Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

Insert

mCh-GluA1-CIB

Expression

Mammalian

Promoter

CaMKII

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-hCHD7

Plasmid

#89458

Purpose

constitutive expression of human CHD7 in mammalian cells

Depositor

Peter Scacheri

Insert

CHD7 (CHD7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt2(WT)

Plasmid

#86623

Purpose

Expresses mCherry-tagged human Akt2(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2 (AKT2 Human)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-YE2-BE3

Plasmid

#85176

Purpose

Expresses YE2-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-JNK2

Plasmid

#86831

Purpose

mammalian expression of GFP-JNK2

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK2 (MAPK9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14

Plasmid

#86747

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-mMaroon

Plasmid

#84486

Purpose

Direct FRET pair Fusion mCyRFP1-mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-mMaroon1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK6 wt

Plasmid

#80882

Purpose

mammalian expression of ALK6 WT

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
DT480

Plasmid

#80413

Purpose

Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FRE5Cf

Plasmid

#62378

Purpose

Derived from FRE5. A two nucleotide insertion to shift the reading frame to dsRED.

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DY2230AA

Plasmid

#64878

Purpose

lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

D2330A,Y2331A, in the DNA polymerase domain (POL)…

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
KRas FAK biosensor

Plasmid

#78301

Purpose

FRET biosensor. Visualization of FAK activity at plasma membrane outside lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

KRas FAK biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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