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qTAG-C-3xFLAG-Blast

Plasmid

#207717

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti NL

Plasmid

#113450

Purpose

Lentiviral NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Lentiviral and Luciferase

Tags

cmyc

Expression

Mammalian

Promoter

hUbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PEmax-P2A-EGFP

Plasmid

#240534

Purpose

For lentiviral expression of PEmax with EGFP

Depositor

Wensheng Wei

Article

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

PEmax-P2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

Plasmid

#104495

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, and AAV1

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

Plasmid

#69908

Purpose

Expresses a GFP circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

RFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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U6-3' MS2-CircRNA

Plasmid

#213751

Purpose

For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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MAP3K5-TAP

Plasmid

#69726

Purpose

Express tandem tagged MAP3K5 in cells

Depositor

Daniel Liebler

Insert

Apoptosis signal-regulating kinase 1 (MAP3K5 Human)

Tags

HA-FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBOB-synP-HTB

Plasmid

#30195

Depositor

Edward Callaway, Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

histone-GFP-2A-TVA receptor-2A-rabies glycoprotein

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-MST2

Plasmid

#166475

Purpose

Expresses fusion of mEGFP and MST2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, MST2 (STK3 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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NLS-Halo

Plasmid

#200197

Purpose

Expression for SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1hSIRT5_HA

Plasmid

#24483

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Sirtuin 5 (SIRT5 Human)

Tags

HA

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP47

Plasmid

#101915

Purpose

Expresses SNAP47 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP47 (SNAP47 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBudORF2-CH

Plasmid

#51289

Purpose

expresses the codon optimized ORF2 of L1RP with his tag

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 ORF2 (L1RE1 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE-SHH

Plasmid

#134350

Purpose

Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

Depositor

Lorenz Studer

Article

Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

Insert

SHH (SHH Human)

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 HaloTag-V5-TurboID

Plasmid

#226474

Purpose

Expresses fusion of HaloTag and TurboID in mammalian cells

Depositor

Hyun-Woo Rhee

Article

Kwak et al Cell Chem Biol. 2022 Dec 15;29(12):1739-

Insert

TurboID

Tags

HaloTag and V5

Expression

Mammalian

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.14

Plasmid

#155034

Purpose

Fluorescent reporter for ATF4 translation, Positive Control

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

uORF1 and uORF2 start codons mutated

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C7orf59

Plasmid

#42332

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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Bcr/Abl P210-pLEF

Plasmid

#38158

Depositor

Nora Heisterkamp

Article

Kweon et al J Biol Chem. 2008 Feb 8;283(6):3023-30.

Insert

BCR/ABL P210 (ABL1, BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NFATC2

Plasmid

#207191

Purpose

doxycycline-inducible expression of Human NFATC2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFATC2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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hSyn1-sypHy-RGECO

Plasmid

#84078

Purpose

Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

Depositor

Juan Burrone

Article

Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

Insert

sypHy-RGECO

Expression

Mammalian

Promoter

hSyn1

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF3173

Plasmid

#144649

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

STAT6 (STAT6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pZFN-AAVS1_ELD

Plasmid

#159297

Purpose

ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

Depositor

Kosuke Yusa

Insert

ZFN targeting the AAVS1 locus

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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78,890 results