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80,555 results

Showing: 1561 - 1600 of 80555 results

  2. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Article
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  19. pFLAG-CMV2-Hsp27-S82A

    Plasmid
    #84993
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 82 mutated to Alanine
    Promoter
    CMV
    Available Since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    84993-plasmid-map-sequence-id-167696

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  21. pB80-SSPB(micro)-GFP-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174640
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-GFP-GCN4-ppKinVIb(861-1321)
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174640-plasmid-map-sequence-id-347051

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  23. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid
    #174630
    Purpose
    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174630-plasmid-map-sequence-id-346973

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  24. pD649-HAsp-VCAM1-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156942
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    VCAM1 (VCAM1 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156942-plasmid-map-sequence-id-389772

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  25. pFLAG-CMV2-Hsp27-S82D

    Plasmid
    #85184
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S82D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85184-plasmid-map-sequence-id-167830

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  34. pEF-GalNAcT2-moxGFP2

    Plasmid
    #218966
    Purpose
    Expresses full length GalNAcT2-moxGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-moxGFP2 (GALNT2 Human)
    Tags
    moxGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218966-plasmid-map-sequence-id-445673

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  39. pAAV-synmiR-L-mRuby-Pro-EGFP-4×17

    Plasmid
    #218262
    Purpose
    Expresses EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-EGFP-MREL_4×17
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    218262-plasmid-map-sequence-id-494309

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  40. pUC57-Homology Arms-BRD4-HaloTag

    Plasmid
    #228575
    Purpose
    CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion
    Depositor
    Marcin Imielinski
    Insert
    Homology Arms-BRD4-HaloTag (BRD4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    228575-plasmid-map-sequence-id-461799

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Showing: 1561 - 1600 of 80555 results