75,820 results

1. pX330-SpCas9-NG

   Plasmid

   #117919

   Purpose

   Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

   Depositor

   Osamu Nureki

   Article

   Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

   Insert

   SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

   Plasmid

   #73208

   Purpose

   Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP-Rab5A Q79L

   Plasmid

   #28046

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab5A (RAB5A Human)
   

   Use

   Tags

   EGFP on N-term

   Expression

   Mammalian

   Mutation

   Q79L

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-TRE3G-PYL1-sfGFP-PML

   Plasmid

   #167011

   Purpose

   expression of PYL1-SfGFP-PML, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   PML (PML Human)
   

   Use

   Lentiviral

   Tags

   GFP-PML

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234436

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSK-mU6-Cj-sgRNA-SV40-puro

   Plasmid

   #192128

   Purpose

   Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Cj-SgRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXLE-hGLIS1

   Plasmid

   #37625

   Purpose

   Non-integrating (episomal) expression of human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   human GLIS1 (GLIS1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T110A, A187G, I221T

   Promoter

   CAG

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-mScarlet-I

   Plasmid

   #159172

   Purpose

   To express a bright monomeric red FP for labelling and detection of mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mScarlet-I
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Codons were optimized to Human codon usage

   Promoter

   CMV

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+) EGFP-RalA(WT)

    Plasmid

    #224274

    Purpose

    Cell transfection and expression of RalA

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    RalA (RALA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZac2.1 gfaABC1D-cyto-GCaMP8s

    Plasmid

    #186052

    Purpose

    AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. RFP Dynamin2 K44A

    Plasmid

    #128153

    Purpose

    Expression of Dynamin 2 K44A

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

    Insert

    Dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-C1(2)delta

    Plasmid

    #21216

    Depositor

    Tobias Meyer

    Article

    Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

    Insert

    GFP-N2-PKCdelta-C1 (Prkcd Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C1(2) domains of PKC delta (rat) (Lab plasmid WC…

    Promoter

    Available since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEBB HA cIAP1 H588A

    Plasmid

    #38233

    Depositor

    Colin Duckett

    Article

    Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

    Insert

    cIAP1 H588A (BIRC2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H588A

    Promoter

    EF1a

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCAGGS_L_MARV

    Plasmid

    #135473

    Purpose

    Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    L gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    silent mutation (C to T) at position 7861; insert…

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. RelB cFlag pcDNA3

    Plasmid

    #20017

    Depositor

    Stephen Smale

    Insert

    RelB (Relb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. PPIB

    Plasmid

    #155838

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPIB (PPIB Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-TIS11B

    Plasmid

    #121157

    Purpose

    Mammalian expression of GFP tagged TIS11B

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    ZFP36L1 (TIS11B) (ZFP36L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid

    #177186

    Purpose

    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Inserts

    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV and U6

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRY2olig-TDP-43

    Plasmid

    #218920

    Purpose

    Expression of CRY2olig-mCherry fused to TDP-43

    Depositor

    Tatyana Shelkovnikova

    Insert

    CRY2olig-mCherry tagged TDP-43
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge