76,966 results

1. pCAG-Cre:GFP

   Plasmid

   #13776

   Purpose

   Mammalian expression of Cre recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-GFP fusion protein
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-parkin C431S

   Plasmid

   #45877

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C431S mutation (ubiquitin acceptor site)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMax_GFP

   Plasmid

   #177825

   Purpose

   Host Cell Reactivation (HCR) control GFP plasmid (pMax backbone)

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3-EF1aH NICD1

   Plasmid

   #86500

   Purpose

   Mammalian expression of Myc tagged NICD1

   Depositor

   Xin Chen

   Article

   Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

   Insert

   Notch1 (C-term) (Notch1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cytoplasmic domain only (AA 1753-2531)

   Promoter

   EF1a

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TRKB-V5/HIS

   Plasmid

   #205614

   Purpose

   Expression of human TRKB receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKB receptor tyrosine kinase (NTRK2 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-CMV-H-sDarken

   Plasmid

   #184800

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. piggyBAC-H2B FR-MQV

   Plasmid

   #194079

   Purpose

   Expresses nuclear-localized FR-MQV in mammalian cells

   Depositor

   Ralph Jimenez

   Article

   Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

   Insert

   FR-MQV
   

   Use

   Tags

   Histone H2B

   Expression

   Mammalian

   Mutation

   Methionine 42 to Glutamine, Cystiene 159 to Valin…

   Promoter

   CMV7

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

   Plasmid

   #131462

   Purpose

   MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
   

   Use

   Cre/Lox and Synthetic Biology; Mosaic analysis fo…

   Tags

   Trp53-V5, H3F3A-EGFP-AU1

   Expression

   Mammalian

   Mutation

   H3F3A K27M, Pdgfra D842V

   Promoter

   none (4x polyA to mitigate episomal expression)

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-10:LMNB1-mEGFP

   Plasmid

   #87422

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   LMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Rtn4b-GFP

    Plasmid

    #186952

    Purpose

    Expresses Rtn4b connected with C-terminus AcGFP1

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN4B (RTN4 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FosB

    Plasmid

    #187908

    Purpose

    Expresses FosB in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    FosB (Fosb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_V5-TWIST1

    Plasmid

    #215030

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLBW cox8 EGFP mCherry

    Plasmid

    #78520

    Purpose

    Fluorescent mitophagy reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    none
    

    Use

    Retroviral

    Tags

    cox8 EGFP mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT_TO_FlagHA_G3BP1

    Plasmid

    #127106

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-TA-ERN

    Plasmid

    #80474

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid

    #58993

    Purpose

    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    EcoRV site in MCS converted to BamHI site

    Promoter

    CMV enhancer-chicken beta actin

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRGFP

    Plasmid

    #26475

    Purpose

    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

    Depositor

    Maria Jasin

    Article

    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

    Insert

    DRGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATeam1.03-nD/nA/pcDNA3

    Plasmid

    #51958

    Purpose

    mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge