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pUCmini-iCAP-PHP.S

Plasmid

#103006

Purpose

non-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

Synthetic construct isolate AAV-PHP.S VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3 (human)

Plasmid

#24920

Purpose

Mammalian expression of LC3 fused to EGFP

Depositor

Toren Finkel

Article

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-RVG-10-Lamp2b-HA

Plasmid

#71294

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

GNSTM glycosylation motif, HA, Lamp2 signal pepti…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176269

Purpose

Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCasMINI-V4-VPR-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM4Di

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGluSnFR4f-NGR-WPRE

Plasmid

#234452

Purpose

AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

Depositor

GENIE Project

Article

Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi

Insert

iGluSnFR4f-NGR

Use

AAV

Tags

IgK chain and Myc epi tag-NGR GPI anchor

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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mEmerald-Sec61b-C1

Plasmid

#90992

Purpose

Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Article

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Insert

mEmerald-Sec61b (SEC61B Synthetic, Human)

Tags

mEmerald (green fluorescence)

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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Lamp1-mScarlet-I

Plasmid

#98827

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-pur H-2Kb

Plasmid

#111623

Purpose

Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

Depositor

Jon Yewdell

Insert

H2Kb

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pDisplay-SNAPtag

Plasmid

#205094

Purpose

Display SNAPtag on the surface of mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

SNAPtag

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Luc

Plasmid

#45968

Depositor

Ralf Kuehn

Insert

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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SFB-SLX4

Plasmid

#247854

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged SLX4

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

SLX4 (SLX4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-NanoLuc

Plasmid

#188116

Purpose

Template plasmid to transcribe circular RNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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HA-DDX3

Plasmid

#44975

Depositor

Robin Reed

Article

Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

Insert

DDX3 (DDX3X Human)

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only

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pV231-xPE

Plasmid

#225257

Purpose

Mammalian expression of xPE

Depositor

Vikash Chauhan, Robert Langer, Phil Sharp

Article

Chauhan et al Nature. 2025 Sep 17. doi: 10.1038/s41586

Insert

xPE

Use

CRISPR

Expression

Mammalian

Mutation

R221K K848A H982A N1317R

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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anxA2-GFP

Plasmid

#107196

Purpose

Expresses human annexin A2-GFP fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

annexin A2 (ANXA2 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

Ala residue 65 in human sequence replaced by Glu …

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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