78,292 results

1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-BI-hGRAD

   Plasmid

   #207837

   Purpose

   Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   mCherry
   hFBXW11 (FBXW11 Human)
   VHH-GFP4
   

   Expression

   Mammalian

   Mutation

   215 amino acids of the full length protein

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHM126

   Plasmid

   #225528

   Purpose

   Encodes Wnt-responsive destablized GFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAX APLP1

   Plasmid

   #30141

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Like Protein 1 (APLP1 Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCMV2 Bxb1-c22

   Plasmid

   #242874

   Purpose

   Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A369P, E434G)

   Depositor

   Jesse Owens

   Article

   Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

   Insert

   Bxb1 Integrase
   

   Expression

   Mammalian

   Mutation

   I87L, A369P, E434G

   Promoter

   CMV

   Available Since

   Sept. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-minCMV-mCherry

   Plasmid

   #27970

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-R-Creon shRNA[Control]

   Plasmid

   #180775

   Purpose

   This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.

   Depositor

   Eun Mi Hwang

   Article

   Kim et al J Neurosci Methods. 2021 Dec 21:109452.

   Type

   Empty backbone

   Use

   AAV, Cre/Lox, and RNAi

   Expression

   Mammalian

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PEA1-Puro

   Plasmid

   #171991

   Purpose

   Delivers all prime editing nickase components in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Expression

    Mammalian

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti MS2-P65-HSF1_Hygro

    Plasmid

    #61426

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Insert

    MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1A

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTreTight-Htt94Q-CFP

    Plasmid

    #23966

    Depositor

    Nico Dantuma

    Article

    Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 1

    Insert

    N-terminal fragment Huntingtin Q94 (HTT Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    94 polyQ repeat

    Available Since

    April 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. mApple-TfR-RUSH

    Plasmid

    #166906

    Purpose

    RUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mApple

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    mApple
    

    Expression

    Mammalian

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-FKBP-MTM1

    Plasmid

    #51614

    Purpose

    Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    FKBP
    MTM1 (MTM1 Human, 1812)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBRPB CAG-mCherry-IP

    Plasmid

    #106333

    Purpose

    PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    mCherry-IRES-Puro
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1 NFAT3

    Plasmid

    #10961

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    NFAT3 (NFATC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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