74,931 results

1. pcDNA5-TOM20_Halo_T2A_EGFP

   Plasmid

   #135443

   Purpose

   Mamalian cell expression of mictochondrial HaloTag7 and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   TOM20-HaloTag7 and EGFP
   

   Use

   Tags

   TOM20

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-Gsdmd-NT 4A

   Plasmid

   #80952

   Purpose

   Expresses Gsdmd-NT 4A mutant in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-NT 4A (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-EGFP (BPK1098)

   Plasmid

   #133962

   Purpose

   CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription

   Depositor

   Benjamin Kleinstiver

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZac2.1 gfaABC1D-cyto-GCaMP8s

   Plasmid

   #186052

   Purpose

   AAV-mediated expression of GCaMP8s under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Eco

   Plasmid

   #35617

   Depositor

   Patrick Salmon, Arthur Nienhuis

   Insert

   Ecotropic MLV envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nuclear cpGFP

   Plasmid

   #215707

   Purpose

   Expresses cpGFP in the nucleus

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nsp7-mCherry

   Plasmid

   #165134

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp7 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC-FURIN

   Plasmid

   #158386

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   furin, paired basic amino acid cleaving enzyme (FURIN Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRluc-LC3wt

    Plasmid

    #105002

    Purpose

    Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

    Depositor

    Marja Jããttelã

    Article

    Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

    Insert

    LC3B (Map1lc3b Rat)
    

    Use

    Luciferase

    Tags

    Rluc with C124A substitution

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHTN-HaloTag_BCL11A:1-773

    Plasmid

    #211014

    Purpose

    Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    BCL11A:M1-F773 (BCL11A Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    isoform 2

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEGFP-C3_SEPT6

    Plasmid

    #71583

    Purpose

    mammalian expression of human SEPT6 with a GFP fusion

    Depositor

    Elias Spiliotis

    Article

    Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

    Insert

    Septin 6 (SEPTIN6 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    V206D

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRH-1187 VSVGmut

    Plasmid

    #201913

    Purpose

    Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    VSV-G (K47Q, R354A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-DIO-tdTomato

    Plasmid

    #72254

    Purpose

    Express tdTomato under EF-1 promoter when Cre is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    tdTomato
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-Sox2

    Plasmid

    #13367

    Purpose

    Retroviral expression of mouse Sox2

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    SRY-box containing gene 2 (Sox2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. c-Fos

    Plasmid

    #187905

    Purpose

    Expresses c-Fos in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    cFos (FOS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-Rab35 S22N inactive

    Plasmid

    #47426

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 S22N (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S22N inactive

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZ M2rtTA_CAGG TetON-Sox10

    Plasmid

    #115240

    Purpose

    RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-PDGFRbeta

    Plasmid

    #136455

    Purpose

    Mammalian expression plasmid for c-myc-tagged PDGFRB

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRbeta (PDGFRB Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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