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4,296 results

Showing: 851 - 900 of 4296 results
  1. pGWB701NL3F10H

    Plasmid
    #141288
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, Tunicamycin R for plant)
    Depositor
    Type
    Empty backbone
    Use
    Luciferase; Gateway
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pYPQ136B2.0

    Plasmid
    #167160
    Purpose
    Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU3
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pYPQ262-ABE8e

    Plasmid
    #161523
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing
    Depositor
    Insert
    ABE8e-zSpCas9(D10A)
    Use
    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pUPD2 hpIntron (GB1281)

    Plasmid
    #160569
    Purpose
    Intron sequence from S. lycopersicum
    Depositor
    Insert
    hpIntron
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pICH47802-p35S::tdTomato-ER::tNOS

    Plasmid
    #160220
    Purpose
    tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse
    Depositor
    Insert
    p2x35S::TdTomato-ER::tNOS
    Use
    Synthetic Biology; Moclo toolkit level 1 acceptor…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pFBP_2xNbLYS1::LUZ

    Plasmid
    #189918
    Purpose
    Binary vector for Agrobacterium-mediated transient expression of a bioluminescent reporter for assessing immune activation in Nicotiana benthamiana by transiently expressed plant immune receptors.
    Depositor
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p2xNbLYS1:NnLuz:tAtuOcs
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Various
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pUB-Cas9

    Plasmid
    #86556
    Purpose
    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)
    Depositor
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Cas9 is codon optimized for C. reinhardtii
    Promoter
    UBIQUITIN10
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pYPQ230-E795L

    Plasmid
    #176890
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter
    Depositor
    Insert
    LbCas12a-E795L
    Use
    CRISPR; Gateway compatible lbcpf1 entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pN094

    Plasmid
    #167155
    Purpose
    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Insert
    p35s-npgA-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. pYPQ210

    Plasmid
    #109329
    Purpose
    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection
    Depositor
    Type
    Empty backbone
    Use
    Gateway compatible attr1-attr2 destination vector
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pDB1alpha11:empty

    Plasmid
    #106207
    Purpose
    GoldenBraid level 1 Alpha plant expression vector.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. pDL691

    Plasmid
    #231166
    Purpose
    T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
    Depositor
    Insert
    ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    PEBV sub-genomic
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. P305-FBP_4

    Plasmid
    #139695
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Insert
    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    Use
    Luciferase
    Tags
    Expression
    Plant
    Mutation
    Promoter
    various
    Available since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Promoter
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. HBT_pcoCASphi_version1

    Plasmid
    #197931
    Purpose
    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal
    Depositor
    Insert
    pcoCasphi-2
    Use
    CRISPR
    Tags
    FLAG tag and SV40 NLS and nucleoplasmin NLS
    Expression
    Plant
    Mutation
    Promoter
    35SPPDK promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  18. GB4070

    Plasmid
    #187811
    Purpose
    Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
    Depositor
    Insert
    copper-inducible dCasEV2.1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. pDB1alpha12:empty

    Plasmid
    #106208
    Purpose
    GoldenBraid level 1 Alpha plant expression vector.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. pDB1alpha13:empty

    Plasmid
    #106209
    Purpose
    GoldenBraid level 1 Alpha plant expression vector.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pDB1alpha14:empty

    Plasmid
    #106210
    Purpose
    GoldenBraid level 1 Alpha plant expression vector.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. pDB1alpha13R:empty

    Plasmid
    #106211
    Purpose
    GoldenBraid level 1 Alpha plant expression vector.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  23. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 851 - 900 of 4296 results