4,625 results
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Plasmid#195938PurposePhiC31-intein(DnaE-Npu) and Intein(DnaE-Ssp)-PhiC31 : pUBQ10-PI-tUBQ1-p35S-IP-t35SDepositorInsertpUBQ10-PI-tUBQ1-p35S-IP-t35S
ExpressionPlantAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT9054
Plasmid#219430PurposeTranscriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCSDepositorInsertLB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
UseCRISPR; Moclo compatible level 1 module (position…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantPromoter2x35SAvailable SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSiM24-eGFP
Plasmid#67883PurposePlant Gene expression Vector pSIM24 containing eGFP reporterDepositorInserteGFP
ExpressionPlantPromoterM24Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB_35S/mEGFP
Plasmid#135320PurposemEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)DepositorInsertmEGFP
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH41331::pU6cm-pegR::EURb7
Plasmid#227695PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).DepositorInsertAltered epegRNA backbone with cloning sites for gRNA and RTT.
UseCRISPRExpressionPlantPromoterU6 compositeAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUbiGUSPlus
Plasmid#64402PurposeUbiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.DepositorInsertubiquitin (Ubi-1) promoter
TagsGUSPlus reporterExpressionPlantPromoterUbi-1Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
L1_lacZgRNA-Ck3
Plasmid#136136PurposeL1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screeningDepositorInsertp5-MpU6:lacZgRNA
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE082 FoMV
Plasmid#234811PurposeTo clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)DepositorTypeEmpty backboneExpressionPlantAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAGT9569
Plasmid#219431PurposeTranscriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOSDepositorInsertLB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsPapE-4LF2ExpressionPlantAvailable SinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneExpressionPlantPromoter2xCaMV35S enhancer and omegaAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA_ASX
Plasmid#98888PurposePlant expression vector for multigene cloningDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterCaMV35S (enhanced)Available SinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLSL.R.Ly
Plasmid#227697PurposeReengineered geminiviral replicon vectorDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantPromoterAtRPS5AAvailable SinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMKV057
Plasmid#133312PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPTDepositorInsertLuc+. WUS2, IPT
ExpressionPlantPromoterAtUbi10, Nos, 35SAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBL101-RUBY
Plasmid#199723PurposeExpresses betalain biosynthesis genes under CaMV 35S promoterDepositorInsert35S-RUBY
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
4784 TRV2-eTnpBc-reRNA4PDS
Plasmid#251816PurposeEditing Photene desaturase (PDS) genes in Nicotiana benthamianaDepositorInsertEnhanced variant of ISDra2 eTnpBc with guide targeting NbPDS with HDV at 3' end
TagsAtFDnlsExpressionPlantMutationN4Y/R110K/V192L/L222IAvailable SinceMarch 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJRA107
Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiFCt-2in1-CC
Plasmid#105114Purposeratiometric BiFC analysis in plants (transient transformation)DepositorTypeEmpty backboneTagscYFP-Myc/nYFP-HAExpressionPlantAvailable SinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRA108
Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YN
Plasmid#74723PurposeExpress C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantPromoterCaMV 35SAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA7001-DEST
Plasmid#71745PurposeGATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducibleDepositorTypeEmpty backboneExpressionPlantPromoterGAL4Available SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
AMBERv1 (pICSL80187)
Plasmid#252515PurposeLevel 0 plasmid containing AMBERv1 CDS (pICSL80187)DepositorInsertAMBERv1
ExpressionPlantAvailable SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
AMBERv2 (pICSL80189)
Plasmid#252516PurposeLevel 0 plasmid containing AMBERv2 CDS (pICSL80189)DepositorInsertAMBERv2
ExpressionPlantAvailable SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
GOLD (pICSL80188)
Plasmid#252517PurposeLevel 0 plasmid containing GOLD CDS (pICSL80188)DepositorInsertGOLD
ExpressionPlantAvailable SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGW-GOI-TurboID
Plasmid#209378PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT56
Plasmid#223428PurposeT-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
4930 TRV2-eTnpBc2xSV40nls-HHreRNA4PDS
Plasmid#251823PurposeEditing Photene desaturase (PDS) genes in Nicotiana benthamianaDepositorInsertEnhanced variant of ISDra2 eTnpBc with guide targeting NbPDS flanked by HH-HDV ribozymes
Tags2xSV40nlsExpressionPlantMutationN4Y/R110K/V192L/L222IAvailable SinceMarch 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT51
Plasmid#223423PurposeT-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.DepositorInsert2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
UseCRISPRTagsNLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-PE2max-NC::tEURb7
Plasmid#227693PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette.DepositorInsertPE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
At2S3:RUBY
Plasmid#160906PurposeRUBY under the control of At2S3 promoter/marker for Arabidopsis transgenesDepositorInsertRUBY and At2S3 promoter
ExpressionPlantPromoterAt2S3Available SinceNov. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL2299A
Plasmid#222105PurposeA ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.DepositorInsertvirA
ExpressionBacterial and PlantPromotervirA promoterAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFZ1035
Plasmid#246938PurposeGmWUS along with RUBY for soybean genome editingDepositorInsertGmWUS, Cas9, RUBY
UseCRISPRExpressionPlantPromoterNOS, AtUbi10Available SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pFZ1036
Plasmid#250676PurposeIPT along with RUBY for soybean genome editingDepositorInsertIPT, Cas9, RUBY
UseCRISPRExpressionPlantPromoterCaMV35, AtUbi10Available SinceFeb. 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCUU
Plasmid#159747PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22C
Plasmid#91135PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB505
Plasmid#74847PurposeGateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAL4BD-VP16/pDON207
Plasmid#201230PurposeGAL4-VP16 transcription factorDepositorInsertGAL4BD-VP16
ExpressionPlantAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-VP16-GWY/pAM
Plasmid#201234PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-VP16-GWY
ExpressionPlantPromoterCaMV 35SAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-GAL4BD-GWY/pAM
Plasmid#201233PurposeUsed to produce and express in planta N-terminal fusion proteins with the GAL4 BDDepositorInsert35S-GAL4BD-GWY
ExpressionPlantPromoterCaMV 35SAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-GAL4BD/pAM
Plasmid#201231PurposeUsed to produce and express in planta N-terminal fusion proteins with the GAL4 BDDepositorInsert35S-GWY-GAL4BD
ExpressionPlantPromoterCaMV 35SAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
35S-GWY-VP16/pAM
Plasmid#201232PurposeUsed to produce and express in planta C-terminal fusion proteins with the VP16 activation domainDepositorInsert35S-GWY-VP16
ExpressionPlantPromoterCaMV 35SAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW-TurboID-GOI
Plasmid#209380PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-ZmUbi-11
Plasmid#197777PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmUbi terminator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
QPM-sgR (pTaU3)
Plasmid#140448PurposeFor sgRNA expression in monocotyledons protoplastsDepositorInsertTaU3-sgRNA
UseCRISPRExpressionPlantPromoterTaU3Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only