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Plasmid#153357PurposeMobius Assembly cloning and expression vector for plants with high-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only
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pECNUS_P2
Plasmid#194344PurposesgRNA position entry clone 2DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P1
Plasmid#194343PurposesgRNA position entry clone 1DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P3
Plasmid#194345PurposesgRNA position entry clone 3DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P4
Plasmid#194346PurposesgRNA position entry clone 4DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P5
Plasmid#194347PurposesgRNA position entry clone 5DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P6
Plasmid#194348PurposesgRNA position entry clone 6DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_P7
Plasmid#194349PurposesgRNA position entry clone 7DepositorInsertsgRNA expression cassettes and LacZ
UseTagsExpressionPlantMutationPromoterAth U6 promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL198
Plasmid#180472PurposeFor expression of Cas9 and gRNAs, multiple gRNAs cloning vectorDepositorTypeEmpty backboneUseCRISPRTagsZmCas9ExpressionPlantMutationPromoterAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB528
Plasmid#74870PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRW006-SM-destination-pro35S-Cas9
Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S::AtSOBIR1-mCherry
Plasmid#86166PurposeSOBIR1-mCherry expressionDepositorInsertSOBIR1 (SOBIR1 Mustard Weed)
UseTagsmCherryExpressionPlantMutationPromoter35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
Plasmid#192380PurposeTo test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MIGS2.1 (FF570)
Plasmid#35247DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)
Plasmid#160622PurposeTU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLLDepositorInsertP35s_MS2:EDLL_Tnos
UseTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH55
Plasmid#125814PurposeLevel0 nSpCas9-PmCDA, Arabidopsis codon optimizedDepositorInsertnSpCas9-PmCDA, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE652
Plasmid#153230PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MASp
Plasmid#153364PurposeAgrobacterium promoter for plant expressionDepositorInsertMAS promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PV225
Plasmid#132334PurposedCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea maysDepositorInsertdCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
UseTagsAT-CBF1ExpressionPlantMutationCodon optimize for expression and stability in Ze…PromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-02
Plasmid#125761PurposeEndlinker for tRNA-gRNA system when using only two guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
Plasmid#106433PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertpUBQ10::ZF1CACTA1_3xFLAG_TET1cd (TET1 Human, Synthetic, Mustard Weed)
UseSynthetic BiologyTags3xFLAGExpressionPlantMutationPromoterAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289 (ErCas12a)
Plasmid#138126PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoterDepositorInsertErCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)13
Plasmid#127526PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNC-Cam2304-35S
Plasmid#193385PurposeNimble cloning-compatible vector for plant ectopic expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.4
Plasmid#158406PurposeExpress single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB326
Plasmid#68654PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_blp
Plasmid#159710PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_Hyg
Plasmid#159709PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTT135
Plasmid#207924PurposePremade UBI10 driven expression of LhG4 (pUBQ10:GR-LhG4:tHSP18.2M) for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP108
Plasmid#207928PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBD001
Plasmid#201330PurposepDefence14:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)DepositorInsertpDefence14:Eluc
UseTagsExpressionPlantMutationremoval of BsaI and BpiI sitesPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB Tnos:NptII:Pnos (GB0226)
Plasmid#68212PurposeTranscriptional unit for kanamycin resistance gene plant expressionDepositorInsertNptII
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v2
Plasmid#138119PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoterDepositorInsertMb2Cas12a v2
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172R, N563R and K569RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM159
Plasmid#84084Purposetarget miRNA159 for destruction in Arabidopsis thalianaDepositorInsertSTTM159
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dAaCas12b-Act3.0
Plasmid#158413PurposeCRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH33
Plasmid#125885PurposeLevel0 Wheat TaU3 Pol III promoter with transcription Start-ADepositorInsertLevel0 Wheat TaU3 Pol III promoter with transcription Start-A
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PDF1::mCitrine-MBD
Plasmid#115222PurposeMicrotubule visualizationDepositorInsertMicrotubule binding domain
UseTagsmCitrineExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORFE0005
Plasmid#112073PurposeAtDeadCAS9 D10A H840A without stop codon module CDS1nsDepositorInsertAtDeadCAS9 D10A H840A without stop codon
UseCRISPRTagsExpressionPlantMutationPlant codon optimized dCas9 D10A S. pyogenesPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
L1_UBE2:mS-Lt-Ck4
Plasmid#136132PurposeL1 in position 4, mark cell outline with mScarletIDepositorInsertp5-MpUBE2:mScarletI-Lti6b_3tNos-35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
3TERM_Nos
Plasmid#136115Purpose3TERM after CDS/ CDS12-CTAGDepositorInsert3TERM_Nos (A. tumefaciens)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUM31dao
Plasmid#87242PurposePlastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plantsDepositorInsertD-amino oxidase
UseTagsExpressionPlantMutationPromoterplastid rrnAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-35S-GW-Luc
Plasmid#80678Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterPro35SAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGWB419
Plasmid#74813PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH56
Plasmid#125882PurposeLevel0 Rice OsACT1 Pol II promoterDepositorInsertLevel0 Rice OsAct1 Pol II promoter
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only